DMSO Effect on Phase Transitions and Vitrification in Cord Blood Erythrocyte Suspension Under 0°C

Authors

  • A. V. Zinchenko
  • E. N. Bobrova
  • M. I. Schetinskiy

Keywords:

differential scanning calorimetry, NMR, DMSO, erythrocytes, cord blood

Abstract

Using the DSC method there were investigated the cord blood erythromass, mixed in 1:1 ratio with DMSO aqueous solutions of different concentrations, as well as the samples of dense sediment and supernatant fluid, obtained after these mixtures’ centrifugation. The temperatures of vitrification, devitrification, crystallisation and melting of eutectic compositions and the temperature of complete melting of systems were determined. There was built a part of diagram for physical states of water-DMSO binary system within the concentration range from 0 up to 60 % (w/w) of DMSO. Using the NMR method it was shown, that the DMSO concentration in supernatant fluid was higher, than in a dense suspension of erythrocytes. This explains the differences in eutectics crystallisation in the samples of erythrocyte dense suspension and in supernatant fluid.

Author Biographies

A. V. Zinchenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

E. N. Bobrova

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

M. I. Schetinskiy

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Downloads

Published

2006-06-25

How to Cite

Zinchenko, A. V., Bobrova, E. N., & Schetinskiy, M. I. (2006). DMSO Effect on Phase Transitions and Vitrification in Cord Blood Erythrocyte Suspension Under 0°C. Problems of Cryobiology and Cryomedicine, (2), 16–21. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1026

Issue

No. 2 (2003): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).