Cryopreservation of Human Embryonic Liver Cells Using DMSO and High Molecular Weight Polymers

Authors

  • Yu. A. Petrenko

Keywords:

human fetal cells, embryonic liver, cryoprotectants, polyethylenoxide

Abstract

In this study an influence of high molecular polymers (Dextran, PEO-1500, PEO-3500, PEO-8000) in combination with different concentrations of DMSO was investigated. It is shown that the adding of 10% dextran into cryopreservation media increased neither survival nor viability rates of FL cells after cryopreservation with all the concentrations of DMSO used. At the same time adding of 10% PEO with different molecular weight facilitated the increasing of survival of FL cells. We can see that survival rate depends on molecular weight of PEOs and has been increased with enhancement of molecular weight. Optimal concentration of PEO-8000 (6%) with minimal concentration of DMSO (2%) was found. In these concentrations of CPAs survival rate was significantly higher than with 10% DMSO alone as well as in the presence of fetal calf serum (FCS).

Author Biography

Yu. A. Petrenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Published

2003-09-25

How to Cite

Petrenko, Y. A. (2003). Cryopreservation of Human Embryonic Liver Cells Using DMSO and High Molecular Weight Polymers. Problems of Cryobiology and Cryomedicine, (3), 80-87. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1063

Issue

Section

Cryopreservation of Biological Resources