Effect of Soybean Seeds Freezing under the Effect of Cryoprotectants on a Sprouting Energy and Germinating Capacity

  • N. O. Shevchenko
  • T. F. Strybul
  • V. V. Zhmurko
  • L. M. Kobyzeva
Keywords: seeds, cryoprotectants, freezing regimens, sprouting energy, germinating capacity

Abstract

We have investigated the effect of soybean seeds freezing down to -55 and -70°C under propanediol (PD), PEO-400 and PEO-1500 cryoprotectants’ effect. There were used various conditions of seeds treatment with cryoprotectants (variation with the concentrations, exposure term), various freezing regimens. It has been established that when selecting the optimum conditions for soybean seeds treatment with cryoprotectants it is possible not only to keep the primary biological characteristics of seeds, but also increase the indices for a sprouting energy and germinating capacity. We have also discussed the reasons for various effect of  cryoprotectants on seeds planting characteristics.

Author Biographies

N. O. Shevchenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

T. F. Strybul

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

V. V. Zhmurko

Kharkov National University named by Karazin V.N.

L. M. Kobyzeva

National Center for Plants Gene Resources of Ukraine, Kharkov

Published
2003-09-25
How to Cite
Shevchenko, N. O., Strybul, T. F., Zhmurko, V. V., & Kobyzeva, L. M. (2003). Effect of Soybean Seeds Freezing under the Effect of Cryoprotectants on a Sprouting Energy and Germinating Capacity. Problems of Cryobiology and Cryomedicine, (3), 92-98. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1065
Section
Cryopreservation of Biological Resources