Integrity of Adult Pig and Newborn Piglets Liver Fragments Under Different Cryopreservation Conditions

Authors

  • B. P. Sandomirsky
  • S. E. Galchenko
  • L. N. Tynynyka

Keywords:

liver fragments, cooling rates, cryoprotectants, endogenous respiration, peroxide processes

Abstract

The effect of cryopreservation factors (cooling rates and cryoprotectants in different concentrations) on the integrity of newborn piglet and adult pig liver fragments was investigated. There was demonstrated the minimum damage of studied biomaterial during cryopreservation on the peroxide processes and the intensity of endogenous respiration when using polyethylene oxides (PEO-400 and PEO-1500) as cryoprotectants with 1 and 8000°C/min cooling rates.

Author Biographies

B. P. Sandomirsky

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

S. E. Galchenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

L. N. Tynynyka

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

Published

2003-12-25

How to Cite

Sandomirsky, B. P., Galchenko, S. E., & Tynynyka, L. N. (2003). Integrity of Adult Pig and Newborn Piglets Liver Fragments Under Different Cryopreservation Conditions. Problems of Cryobiology and Cryomedicine, (4), 77-84. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1082

Issue

Section

Cryopreservation of Biological Resources