EPR-Study of Redox Reactions of Human Placenta Aqueous-Saline Extracts: Storage Temperature and Extraction Time Effects

Authors

  • S. V. Repina
  • O. A. Nardid
  • E. D. Rozanova
  • L. V. Tsymbal
  • M. I. Schetinsky
  • E. I. Naumenko

Keywords:

human placenta extracts, EPR of spine probes, freezing, protein-nucleotide composition, redox reactions

Abstract

Using EPR method there were investigated redox reactions of stable TEMPON nitroxyl radical with human placenta aqueoussaline extracts depending on placenta tissue storage temperature and extraction time. It was demonstrated, that with an increase in extraction time from 2 to 12 hrs, as well as placenta tissue storage at –20°C for a month, there was a decrease in redox process activity in extracts. Freezing of placenta tissue down to –196°C did not significantly affect redox activity of extracts in comparison with those from native placenta. Investigation results of protein-nucleotide composition in the obtained aqueous-saline extracts testify to the fact, that high-molecular extract fragments possess higher reductive activity in respect of TEMPON.

 

Author Biographies

S. V. Repina

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

O. A. Nardid

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

E. D. Rozanova

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

L. V. Tsymbal

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

M. I. Schetinsky

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

E. I. Naumenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

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Published

2005-12-25

How to Cite

Repina, S. V., Nardid, O. A., Rozanova, E. D., Tsymbal, L. V., Schetinsky, M. I., & Naumenko, E. I. (2005). EPR-Study of Redox Reactions of Human Placenta Aqueous-Saline Extracts: Storage Temperature and Extraction Time Effects. Problems of Cryobiology and Cryomedicine, 15(4), 591–598. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1207

Issue

Section

Theoretical and Experimental Cryobiology