Effect of Aluminum Chloride and Different Cryoprotectants on Hypertonic Cryohemolysis Development in Human Erythrocytes

Ya. O. Nardid, N. A. Danilenko, V. V. Ramazanov, V. A. Bondarenko

Abstract


The peculiarities of development of hypertonic cryohemolysis of human erythrocytes at cooling from 37 down to 0°Сunder the influence of different cryoprotectors and aluminum chloride were investigated. It was istablished, that the presence of PEG-1500 and also aluminum chloride in the medium noticeably enhanced the stability of cells to temperature-osmotic effects. The use of penetrating cryoprotectors leads to increasing of hypertonic cryohemolysis level at the second stage of incubation in hypertonic conditions.


Keywords


erythrocytes; hypertonic cryohemolysis; cryoprotectant

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