Effect of Aluminum Chloride and Different Cryoprotectants on Hypertonic Cryohemolysis Development in Human Erythrocytes

  • Ya. O. Nardid
  • N. A. Danilenko
  • V. V. Ramazanov
  • V. A. Bondarenko
Keywords: erythrocytes, hypertonic cryohemolysis, cryoprotectant

Abstract

The peculiarities of development of hypertonic cryohemolysis of human erythrocytes at cooling from 37 down to 0°Сunder the influence of different cryoprotectors and aluminum chloride were investigated. It was istablished, that the presence of PEG-1500 and also aluminum chloride in the medium noticeably enhanced the stability of cells to temperature-osmotic effects. The use of penetrating cryoprotectors leads to increasing of hypertonic cryohemolysis level at the second stage of incubation in hypertonic conditions.

Author Biographies

Ya. O. Nardid

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

N. A. Danilenko

V.N. Karazin Kharkiv National University

V. V. Ramazanov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

V. A. Bondarenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

Published
2005-12-25
How to Cite
Nardid, Y. O., Danilenko, N. A., Ramazanov, V. V., & Bondarenko, V. A. (2005). Effect of Aluminum Chloride and Different Cryoprotectants on Hypertonic Cryohemolysis Development in Human Erythrocytes. Problems of Cryobiology and Cryomedicine, 15(4), 607-613. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1209
Section
Theoretical and Experimental Cryobiology