Effect of Cryoprotective Media Components on Dog Spermatozoa Viability During

Authors

  • T. P. Linnik
  • M. I. Egorov
  • T. S. Dyubko
  • A. P. Belonozhko

Keywords:

spermatozoa, motility, cryopreservation, cryoprotectants, albumin, egg yolk

Abstract

There was studied the effect of such cryoprotectants (CP) as: glycerol (GL), ethylene glycol (EG), dimethyl sulfoxide (DMSO), N-N-dimethyl formamide (DMF), 1,2-propanediol (1,2-PD) in 1 M concentration on dog spermatozoa (DS) viability before and after freeze-thawing in 3 media, differing by composition. The first medium (TFY) comprised the yolk of chicken eggs, the second one (ТFA) consisted of egg albumin, the third one (ТF) was used without additives. It was established, that the highest viability of DS both before and after cryopreservation was observed under DMF protection in TFA medium. A possible mechanism of egg albumin effect on DS viability in combination with DMF is discussed.

Author Biographies

T. P. Linnik

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

M. I. Egorov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

T. S. Dyubko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

A. P. Belonozhko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Published

2005-12-25

How to Cite

Linnik, T. P., Egorov, M. I., Dyubko, T. S., & Belonozhko, A. P. (2005). Effect of Cryoprotective Media Components on Dog Spermatozoa Viability During. Problems of Cryobiology and Cryomedicine, 15(4), 645-656. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1214

Issue

Section

Cryopreservation of Biological Resources