Cryoprotective Efficiency of Some Cryoprotectants Depending on Cooling Rate

  • N. A. Chernobai Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. V. Pakhomov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
Keywords: cryopreservation, cooling rate, testes interstitial cells, permeability, cryoprotectants

Abstract

The survival of testes interstitial cells after freeze-thawing using the cooling rates of 1; 5 and 10 deg/min and glycerol (7%), dimethyl sulfoxide (10%), ethylene glycol (7%) and dimethyl formamide (5%) solutions has been estimated. Using of glycerol provided sufficiently high (up to 75%) cell survival under all the studied cooling rates. Cell survival increased (from 56% upto 83%) if cooling rates were elevated from 1 up to 10 deg/min in the case of other cryoprotectants. Cooling rates about 10 deg/min provided sufficiently high level of post-thaw cell survival.

Published
2011-09-19
How to Cite
Chernobai, N. A., Gurina, T. M., & Pakhomov, A. V. (2011). Cryoprotective Efficiency of Some Cryoprotectants Depending on Cooling Rate. Problems of Cryobiology and Cryomedicine, 21(3), 273-279. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/125
Section
Theoretical and Experimental Cryobiology