Cryoprotective Efficiency of Some Cryoprotectants Depending on Cooling Rate
Keywords:cryopreservation, cooling rate, testes interstitial cells, permeability, cryoprotectants
The survival of testes interstitial cells after freeze-thawing using the cooling rates of 1; 5 and 10 deg/min and glycerol (7%), dimethyl sulfoxide (10%), ethylene glycol (7%) and dimethyl formamide (5%) solutions has been estimated. Using of glycerol provided sufficiently high (up to 75%) cell survival under all the studied cooling rates. Cell survival increased (from 56% upto 83%) if cooling rates were elevated from 1 up to 10 deg/min in the case of other cryoprotectants. Cooling rates about 10 deg/min provided sufficiently high level of post-thaw cell survival.
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