Morphological Features of Thyroid Gland in Rats with Experimental Autoimmune Thyroiditis After Administering Cryopreserved Allogeneic Fetal Cells

Yuriy I. Karachentsev, Natalya G. Malova, Irina V. Komarova, Larisa A. Sirotenko, Larisa Yu. Sergienko, Olga V. Ochenashko, Aleksandr Yu. Petrenko


The designing of novel approaches and search for more efficient means to normalize the thyroid gland (TG) function in autoimmune thyroiditis (AIT) is a topical task in current endocrinology. Here we assessed the effect of cryopreserved allogeneic fetal liver cells (cFLCs) and those of fetal mesodermal tissues (cFMTCs) on thyroid gland (TG) morphostructure in rats with experimental AIT. The cFLCs and cFMTCs were demonstrated to positively affect the TG of rats with induced AIT even at the early stages of the study. Both types of fetal cells potentiated the thyrocyte proliferation and microfollicle differentiation even in 7 days after administration. This effect persisted during a month of observation. Herewith the cells of mesodermal origin had a more pronounced effect than the fetal liver ones. Our findings testify to the prospects of using stem and progenitor cells of fetal origin for AIT correction, that may be the basis in designing a novel efficient approach to the therapy of autoimmune damage of thyroid parenchyma.

Probl Cryobiol Cryomed 2017; 27(4): 356-366


experimental autoimmune thyroiditis; histostructure of thyroid gland; cryopreservation; transplantation; liver cells; fetal cells; mesodermal cells

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Afanasyev Yu.A., editor. Histology. 5th ed. Moscow: Meditsina; 2002. PubMed

Aksoy D.Y. Effects of 12 months treatment with L-selenomethionine on serum anti-TPO levels in patients with Hashimoto's thyroiditis. Thyroid 2007; 17(7): 609–612.">CrossRef PubMed

Benvenga S. Thyroid nodules and thyroid autoimmunity in the context of environmental pollution. Rev Endocr Metab Disord 2015; 16(4): 319–340. CrossRef PubMed

Boelaert K. Thyroid hormone in health and disease. J Endocrinol 2005; 187(1): 1–27. CrossRef PubMed

Brenta G. Comparative efficacy and side effects of the treatment of euthyroid goiter with levo-thyroxine or triiodthyroacetic acid. J Clin Endocrinol Metabol 2003; 88(11): 5287–5292. CrossRef PubMed

Bronshtein M.E. Morphological variants of autoimmune diseases. Problems of Endocrinology 1991; 7(2): 610.

Campbell A., Brieva T., Raviv L. et al. Concise review: process development concsiderations for cell therapy. Stem Cells Transl Med 2015; 4(10): 1155–1163. CrossRef PubMed

Colter D.C. Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc Natl Acad Sci USA 2001; 98: 7841–7845. CrossRef PubMed

De Vito P., Incerpi S., Pedersen J.Z. et al. Thyroid hormones as modulators of immune activities at the cellular level. Thyroid 2011; 21(8): 879–890. CrossRef PubMed

Di Nicola M., Carlo-Stella C., Magni M. et al. Human bone marrow stromal cells suppress T-lymphocyte proliferation induced by cellular or nonspecific mitogenic stimuli. Blood 2002; 99(10): 3838–3843. CrossRef PubMed

Fallahi P., Ferrari S.M., Elia G. et al. Novel therapies for thyroid autoimmune diseases. Expert Rev Clin Pharmacol 2016; 9(6): 853–861. CrossRef PubMed

Fallahi P., Ferrari S. M., Ruffilli I. et al. The association of other autoimmune diseases in patients with autoimmune thyroiditis: Review of the literature and report of a large series of patients. Autoimmun Rev 2016; 15(12): 1125–1128. CrossRef PubMed

Gao F., Chiu S.M., Motan D.A.L., et al. Mesenchymal stem cells and immunomodulation: current status and future prospects. Cell Death and Disease 2016; 7(1): 2062–2066. CrossRef PubMed

Grischenko V.I., Goltsev A.N. Transplantation of the products of embryofetoplacental complex. From understanding of mechanism of the effect to increasing the efficiency of application. Probl Cryobiol Cryomed 2002; (1): 54–84.

Hutchings P., Hedlund G., Dawe K. et al. Effect of the synthetic immunomodulator Linomide on experimental models of thyroiditis. Immunology 1999; 96(3): 340–347. CrossRef PubMed

Incerpi S., Hsieh M.T., Lin H.Y. et al. Thyroid hormone inhibition in L6 myoblasts of IGF-I-mediated glucose uptake and proliferation: new roles for integrin αvβ3. Am J Physiol Cell Physiol 2014; 307(2): 150–161. CrossRef PubMed

Jara L.J., Navarro C., Carrasco M. et al. Thyroid disease in Sjogren's syndrome. Clin Rheumatol 2007; 26(10): 1601–1606. CrossRef PubMed

Jara L.J., Navarro C., Medina G. et al. Immune-neuroendocrine interactions and autoimmune diseases. Clin Dev Immunol 2006; 13(2–4): 109–123. CrossRef PubMed

Kaminsky A.B. Topical questions of therapy of chronic autoimmune thyroiditis therapy: application of selective non-steroidal antiinflammatory drugs. Ukrainian Medical Journal 2004; (4): 40–48.

Kolypetri P., King J., Larijani M. et al. Genes and environment as predisposing factors in autoimmunity: acceleration of spontaneous thyroiditis by dietary iodide in NOD.H2(h4) mice. Int Rev Immunol 2015; 34(6): 542–56. CrossRef

Korbling M., Estrov Z. Adult stem cells for tissue repair – a new therapeutic concept? N Engl J Med 2003; (349): 570–582. CrossRef PubMed

Lavin N., editor. Endocrinology. Moscow: Praktika; 1999.

Malova N.G., Bozhko T.S., Komarova I.V. et al. Influence of placental bioprecipitate on thyroid function of rabbits with experimental hypothyroidism. In: Fundamental and clinical endocrinology: problems, achievements, perspectives (8th Danilevsky Readings). Proceedings Conference with International Participation; 2009 Febr 26–27; Kharkiv. p. 80–81.

Malova N.G., Yurchenko T.M., Bozhko T.S. et al. Experimental substantiation for applying cryopreserved preparations of embryofetoplacental complex in thyroid gland pathology correction. Report I. Effect of fetal thymus preparations on rabbit thyroid gland functional activity. Probl Cryobiol Cryomed 2007; 17(3): 290–297.

Malova N.G., Yurchenko T.M., Bozhko T.S. et al. Experimental substantiation for applying cryopreserved preparations of embryofetoplacental complex in thyroid gland pathology correction. Report 2. The effect of human fetal cell suspension biopreparation on functional activity of rabbit thyroid gland. Probl Cryobiol Cryomed 2007; 17(4): 403–409.

Mansouri A., Chowdhury K., Gruss P. Follicular cells of the thyroid gland require Pax8 gene function. Nat Genet1998; 19(1): 87–90. CrossRef PubMed

Marta E., Montesinos J.J. Immunoregulation by mesenchymal stem cells: biological aspects and clinical applications. Neural Regen Res 2014; 9(24): 2197–2204.

Matvienko A.V. Morphological research at pre-clinical study of drugs. In: Stefanov O.V., editor. Pre-clinical study of drugs: guidelines. Kyiv; 2001. p. 196–199.

Petrenko A.Yu., Sukach A.N. Isolation of intact mitochondria and hepatocytes using vibration. Analytical Biochem 1991; 194(2): 326–329. CrossRef

Petrenko Yu.A. Immunoregulative properties of human fetal hepatic cells. Genes & Cells 2007; 2(3): 57–61.

Rose N.R, Twarog F.J., Crowle A.J. Murine thyroiditis: importance of adjuvant and mouse strain for the induction of thyroid lesions. J Immunol 1971; 106(3): 698–704. PubMed

Sarkisov D.S. Microscopic technique. Moscow: Meditsina; 1996.

Shioko K. Thyroid regeneration: how stem cells play a role? Front Endocrinol (Lausanne) 2014; 5(55): 89–96. CrossRef

Starkova N.N., editor. Guidelines for clinical endocrinology. St. Petersburg: Piter; 1996.

Vyazov O.Ye., editor. Laboratory research methods in noninfectious immunology. Moscow: Meditsina; 1967. PubMed

Weetman A.P. The immunopathogenesis of chronic autoimmune thyroiditis one Century after Hasimoto. Eur Thyroid J 2012; (1): 243–250. PubMed

Wiersinga W.M. Thyroidautoimmunity.Endocr Dev 2014; 26: 139–157. CrossRef PubMed



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