Function of Ovarian Tissue Grafts After Hypothermic Storage: Importance of Incubation Medium Composition
DOI:
https://doi.org/10.15407/cryo29.01.019Keywords:
hypothermic storage, morphology, follicles, sex hormones, mannitol, ovarian tissueAbstract
There were comparatively analyzed the morphology, endocrine function and folliculogenesis of mature and neonatal rat ovarian tissue grafts after hypothermic storage (HS) in the media with different composition. For research performance the following methods were used: heterotopic transplantation (study of ovarian tissue structure and function after HS); immunoassay (determining the concentration of sex hormones in plasma); morphological method (evaluation of tissue graft structure integrity after HS). The composition of incubation medium was experimentally proven to be a key factor in follicular development and endocrine function of ovarian tissue grafts of different stages of histogenesis after HS. It was found that the use of phosphate-buffered saline for HS significantly reduced the follicular pool and decreased the steroidogenic function of grafts in both mature and neonatal ovarian tissue. It was shown that when using the mannitol-containing solution for HS, the number of follicles (of different maturity degree and corpus luteum) and the endocrine function after ovarian tissue transplantation were comparable with those after fresh tissue transplantation.
Probl Cryobiol Cryomed 2019; 29(1): 019–027
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References
Abir R, Biron-Shental T, Orvieto R, et al. Transplantation of frozen–thawed late-gestational-age human fetal ovaries into immunodeficient mice. Fertility and Sterility. 2009; 92(2): 770–7. CrossRef
Andrews RJ, Bringas JR, Muto RP. Effects of mannitol on cerebral blood flow, blood pressure, blood viscosity, hematocrit, sodium, and potassium. Surg Neurol. 1993; 39(3): 218–22. CrossRef
Carvalho FCA, Lucci CM, Silva JRV, et al. Effect of BraunCollins and saline solutions at different temperatures and incubations times on the quality of goat preantral follicles preserved in situ. Anim Reprod Sci. 2000; 66(3): 195–208.
Gastal GDA, Alves BG, Alves KA, et al. Ovarian fragment sizes affect viability and morphology of preantral follicles during storage at 4°C. Reprod. 2017; 153(5): 577–87. CrossRef
Giwa S, Lewis JK, Alvarez L, et al. The promise of organ and tissue preservation to transform medicine. Nat Biotechnol. 2017; 35(6):530–42. CrossRef
Gougeon A. Dynamics of follicular growth in the human: a model from preliminary results. Hum Reprod. 1986; 1(2): 81–7. CrossRef
Guiberta EE, Petrenko AY, Balaban CL, et al. Organ preservation: current concepts and new strategies for the next decade. Transfus Med Hemother. 2011; 38(2): 125–42. CrossRef
Hirshfield AN, DeSanti AM. Patterns of ovarian cell proliferation in rats during the embryonic period and the first three weeks postpartum. Biol Reprod. 1995; 53(5): 1208–21. CrossRef
Israely T, Nevo N, Harmelin A, et al. Reducing ischaemic damage in rodent ovarian xenografts transplanted into granulation tissue. Human Reproduction. 2006; 21(6): 1368–79. CrossRef
Jensen AK, Macklon KT, Fedder J, et al. 86 successful births and 9 ongoing pregnancies worldwide in women transplanted with frozen-thawed ovarian tissue: focus on birth and perinatal outcome in 40 of these children. J Assist Reprod Genet. 2017; 34(3): 325-36. CrossRef
Karibe H, Zarow GJ, Weinstein PR. Use of mild intraischemic hypothermia versus mannitol to reduce infarct size after temporary middle cerebral arteryocclusion in rats. J Neurosurg. 1995; 83(1): 93–8. CrossRef
Kiroshka VV, Medinets EA, Tischenko YuO, Bondarenko TP. Dynamics of morphological change in neonatal ovarian tissue during hypothermic storage depending on composition of incubation medium. Probl Cryobiol Cryomed. 2012; 22(1): 61–70.
Korzhevsky DE, Gilyarov AV. [Fundamentals of histological techniques]. St. Petersburg: SpetsLit; 2010. 95 p. Russian.
Lucci C, Kacinskis M, Rumpf R. Effects of lowered temperatures and media on short-term preservation of zebu (Bosindicus) preantral ovarian follicles. Theriogenology. 2004; 61(2–3): 461–72. CrossRef
Magovern GJ, Belling SF, Casale AS, et al. The mechanism of mannitol in reducing ischemic injury: hyperosmolarity or hydroxyl scavenger. Circulation. 1984; 70(3 Pt 2): 191–5.
Matson BA, Albertini DF. Oogenesis: chromatin and microtubule dynamics during meiotic prophase. Mol Reprod Dev. 1990; 25(4): 374–83. CrossRef
Oredsson S, Plate G, Qvarfordt P. The effect of mannitol on reperfusion injury and postischaemic compartment pressure in skeletal muscle. Eur J Vasc Surg. 1994; 8(3): 326–31. CrossRef
Paczynski RP, He YY, Diringer MN, Hsu CY. Multiple-dose mannitol reduces brain water content in a rat model of cortical infarction. Stroke. 1997; 28(7): 1437–43. CrossRef
Pyatikop VA, Karamyshev VD, Shevereva VM, Dvortsevoy VK. Histological analysis of changes in brain tissues of rats with experimental Parkinson disease prior to and after transplantation of cryopreserved embryonic nerve cells. Problems of Cryobiology. 2006; 16(2): 211–6.
Pyatikop VA, Schegelskaya EA, Mikulinsky YuE, et al. Restoration of structural and functional parameters in rats with cryogenic brain injury after transplantation of bone marrow stromal cells induced into neuroblasts. Problems of Cryobiology. 2005; 15(3): 449–51.
Sağsöz N, Kisa U, Apan A. Ischaemia-reperfusion injury of rat ovary and the effect of vitamin C, mannitol and verapamil. Hum Reprod. 2002; 17(11): 2972–6. CrossRef
Salama M, Isachenko V, Isachenko E, et al. Advances in fertility preservation of female patients with hematological malignancies. Expert Rev Hematol. 2017; 10(11): 951–60. CrossRef
Snow M, Cleary M, Cox S-Let, al. Comparison of the effects of in vitro and in situ storage on the viability of mouse ovarian tissue collected after death. Reprod Fertil Dev. 2001; 13(5-6): 389–94. CrossRef
'THart NA, Leuvenink HGD, Ploeg RJ. New solutions in organ preservation. Transplantation Reviews. 2002; 16(3): 131–41. CrossRef
Wright CS, Becker DL, Lin JS, Warner AE, Hadry K. Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation. Reprod. 2001; 121(1): 77–88. CrossRef
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