Cryopreservation as a Method to Preserve Some Fruit and Berry Crops and Wild Medicinal Plants

Alexander V. Pavlov, Vladimir G. Verzhuk, Svetlana Yu. Orlova, Olga Ye. Radchenko, Maria V. Yerastenkova, Aleхandra Sh. Dodonova, Yelena A. Gavrilkova, Maksim N. Sitnikov, Galina I. Filipenko, Sergey V. Murashev

Abstract


The cryopreservation eff ect on viability of black currant cuttings (Ribes nigrum L.), sweet cherry pollen (Cerasus avium L. Moench), Prunus rossica Erem. diploid plum and Prunus domestica L. garden plum pollen, as well as the seeds of wild medicinal plants: Syrian rue (Peganum harmala L.), tansy (Tanacetum ulutavicum Tzvel.) and sawwort (Serratula dissecta Ledeb.) was studied. The viability in 5 cultivars of R. nigrum L. was decreased after cryopreservation; in 7 cultivars of C. avium L. (17 cultivars totally) it was increased, in 6 cultivars there were no changes, in 4 ones it was decreased; in 3 varieties of P. rossica Erem. (9 cultivars totally) the one was increased, in 3 ones there were no changes, in 3 ones it decreased; in 6 cul-tivars of P. domestica L. (13 cultivars totally) this index was increased, in 3 ones there were no changes, in 4 ones it decreased; in P. harmala L. seeds it was unchanged; in the seeds of S. dissecta Ledeb. it was increased. For the seeds of three medicinal plants there were selected the optimal concentrations of cryoprotectants and thawing modes as follows: P. harmama L. – 100% glycerol, slow freeze-thawing; T. ulutavicum Tzvel. – 10% sucrose, slow warming; S. dissecta Ledeb. – 3% DMSO, rapid warming.

Probl Cryobiol Cryomed 2019; 29(1): 044–057

 


Keywords


cryopreservation; vegetative shoots; pollen; seeds; cryoprotectants

Full Text:

PDF

References


Angaskieva AS, Andreeva VY, Kalinkina GI, et al. [Researching chemical composition of Serratula coronata cultivated in Siberia]. Khimija Rastitel’nogo Syr’ya. 2003; (4): 47–50. Russian.

Belous AM, Grischenko VI. [Cryobiology]. Kyiv: Naukova dumka;1994. 430 p. Russian.

Daletskaya TV, Polyakova EN. [The effect of cryopreservation on seed germination and some aspects of metabolism.] Biofizika Zhivoy Kletki. 1994; 6: 81–5. Russian.

Dzyubenko NI. [Vavilov’s collection of cultivated plants: history and modernity] In: Preservation of biological diversity in Russia as a base for sustainable development and high technologies]. Moscow: GNU VSTISP Rosselkhozakademii; 2011. p. 80–109. Russian.

Dodonova ASh, Gavrilkova EA, Verzhuk VG. [Cryostorage of Serratula dissecta seed material]. In: [Biological features of medicinal and aromatic plants and their role in medicine. Collection of scientific papers of the international scientific-practical conference dedicated to the 85th anniversary of VILAR]. 2016 June 23-25, Moscow, Russia. Moscow: FGBNU VILAR; 2016. р. 46–9. Russian.

Fedosenko VA. [Use of ultralow temperatures for long-term seed storage (methods and practices)]. Bulletin VIR. 1978; 77: 53–7. Russian.

Forsline F, Towill LE, Waddell JW, et al. Recovery and longevity of cryopreserved dormant apple buds. Amer Soc Hort Sci. 1998; 123(3): 365–70. CrossRef

Gavrilkova EA, Dodonova A Sh, Verzhuk VG, et. al. [Low-temperature preservation of Peganum harmala seed material.] Bulletin of the Karaganda University. Biology. Medicine. Geography Series. 2015. (4): 17–23. Russian.

Grischenko VI. [Achievements and prospects of cryobiology development in Ukraine.] Problems of Cryobiology. 2005; 15(3): 231–40. Russian.

James ER. [Сell storage at low temperatures.] In: [Mantell SH., Smith H., editors. Plant biotechnology. 1983.].Translated from English. Moscow: Agropromizdat; 1987. p. 153-75. Russian.

Kiseleva AA, Verzhuk VG, Savelyev NI, et al. [Methods for monitoring genetic stability in fruit crop plants under cryopreservation]. Proceedings on Applied Botany, Genetics and Breeding. 2012; 169: 280–8. Russian.

Maltseva MV. [Handbook on determining sowing qualities of medicinal plant seeds] Moscow: Medgiz; 1950. 56 p. Russian.

Molodkin VY. [Significance of seed moisture content in some cereals and grain legumes during their cryopreservation in liquid nitrogen]. Bulleten VIR 1986; 165: 22–4. Russian.

Mukhitdinov NM., Parshina GN. [Medicinal plants.] Almaty: Kazak Universiteti; 2002. 313 p. Russian.

Omura M, Sato Y, Seike K. Long-term preservation of Japanese pear seeds under extra-low temperatures. In: Akihama T, Nakajama K, editors. Long-term Preservation of Favourable Germplasm in Arboreal Crops. Rome: IBPGR; 1978. p. 26–30.

Pavlov NV. [The flora of Kazakhstan]. Alma-Ata: Izdatelstvo Akademii Nauk Kazakhstana; 1960. Vol.9. 639 p. Russian.

Pegg DE. Principles of cryopreservation. Methods Mol Biol. 2007; 368:39-57. CrossRef

Pegg DE. Principles of cryopreservation. Methods Mol Biol. 2015; 1257:3-19. CrossRef

Popov AS. [Сryopreservation of cultured cells]. In: Pinayev GP., Bogdanova MS, editors. [Methods for cell culturing]. St. Petersburg: Izdatel’stvo Politekhnicheskogo Universiteta; 2008. p. 236-50. Russian.

Reed BM. Plant cryopreservation: a practical guide. New York, NY: Springer; 2008. 532 p. CrossRef

Safina GF, Burmistrov LA. [Low-temperature and cryogenic storage of pear-tree (Pyrus L.) seeds]. Tsitologiya. 2004; 46(10): 851. Russian.

Sakai A, Noshiro M. Some factors contributing to the survival of crop seeds cooled to the temperature of liquid nitrogen. In: Frankel H, Hawkes JG, editors. Crop genetic resources for today and tomorrow. Cambridge; New York: Cambridge University Press; 1975. p. 317-26.

Stanwood P, Bass L. Ultracold preservation of seed germplasm. In: Li PH, Sakai A, editors. Plant cold hardiness and freezing stress. Mechanisms and crop implications. Cambridge: Academic press, Inc.; 1978.Vol.1; p. 361-71. CrossRef

Tikhonova NG, Filipenko GI, Verzhuk VG, et al. Strategy and methods of long-term storage of gene fond of plants. Problems of Cryobiology. 2008; 18 (2): 227.

Udolskaya NL. [Methodology of biometric calculations.] Alma-Ata: Nauka; 1976. 45 p. Russian.

Verzhuk, VG, Pavlov AV, Murashev SV, et al. [The regulating role of cryoprotectants and antioxidants during the storage of fruit and berry plant germplasm.] Mezhdunarodnyy Nauchno-Issledovatelskiy Zhurnal. 2016; (11 Pt 2): 115–8. Russian.

Verzhuk VG, Zhestkov AS, Kotov VM, et al. Perspectives of using cryopreservation for pear (Pyrus L.) shoots preserved in liquid nitrogen vapours. Problems of Cryobiology. 2012; 22(3): 255.

Verzhuk VG, Pavlov AV, Dzyubenko NI, et al. [Cryopreservation in liquid nitrogen as a promising method of preserving the plant biodiversity of drupe and pome fruit crops]. Plodovodstvo I Yagodovodstvo Rossii. 2017; 48(1): 33–6. Russian.

Verzhuk VG, Filipenko GI, Safina GF, et al. [Cryoconservation as an effective method of preserving fruit and berry plant genetic resources]. Proceedings on Applied Botany, Genetics and Breeding. 2012; 169: 270–9. Russian.

Verzhuk VG, Filipenko GI, Pavlov AV, et al. [The effect of light and sucrose concentration in the germination medium on apple-tree pollen germination]. The Way of Science. 2016; 3(25): 14–9. Russian.

Verzhuk, VG, Pavlov AV, Tikhonova OA, et al. Evaluation of fruit plant germplasm’s viability after cryopreservation in liquid nitrogen vapor at 183-185°С]. In: Kunakh VA, editor. [Factors of experimental evolution of organisms]. Kyiv.Logos; 2013. Vol. 13; p. 27-30. Russian.

Yakovlev G.P, Blinova KF. [Medicinal plant materials. Pharmacognosy]. St. Petersburg: Spetsialnaya Literatura; 2004. 763 p. Russian.

Zamburova DS, Sherieva SA, Sitnikov MN, et al. [Studying viability of fruit crop pollen after exposure to ultralow temperatures]. Sovremennye Problemy Nauki i Obrazovaniya. [Internet] 2016[Cited 10.10.2018]; (3):414. Available from: https://www.science-education.ru/ru/article/view?id=24849. Russian.

Zorina MS, Kabanov SP. [Determining seed productivity and seed quality in newly introduced plants]. In: [Techniques of introduction studies in Kazakhstan]. Collection of research papers. Alma-Ata: Nauka; 1976. p.75–85. Russian.

Wusterman MC, Boylan S, Pegg DE. Cryopreservation of rabbit corneas in dimethyl sulfoxide. Invest Ophthalmol Vis Sci. 1997: 38(10):1934-43.




DOI: https://doi.org/10.15407/cryo29.01.044

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

Institute for Problems of Cryobiology and Cryomedicine

23, Pereyaslavskaya str., Kharkov, Ukraine

Tel. +38057 373 4143; Fax +38057 373 5952

e-mail: journal@cryo.org.ua