Influence of Cryopreserved Human Placental Tissue on Reparative Bone Formation in Rats with the Lower Jaw Open Fracture on Osteoporosis Background

Oleksii O. Likhitskyi, Anatoliy M. Goltsev

Abstract


The influence of calcium citrate and cryopreserved human placental tissue reparative osteogenesis in rats with an open fracture of the lower jaw on the background of osteoporosis was investigated. The studies were performed
in three groups (n = 35 each) of mature Wistar male rats (180–200 g weight): the rats with a simulated open mandibular fracture on the osteoporosis background, rats with simulated pathology, which implanted with the cryopreserved human placental tissue and the rats with a simulated pathology, which in addition to an implantation of the cryopreserved human
placental tissue were also injected with calcium citrate. Experimental osteoporosis in rats was induced by injecting of 60% solution of hydrocortisone acetate for 60 days in a dose of 50 mg/kg every 24 hours. Afterwards traumatic damage to the lower jaw was simulated. The model showed that cryopreserved placental tissue had an osteoprotective effect, which was manifested in enhancing the collagen formation processes (maximum to day 30), as well as bone mineralization, depriving the effect on osteo-destructive processes and collagen catabolism (maximum to day 14). The combined use of cryopreserved human placenta and calcium citrate had a powerful osteoprotective effect, which also developed at the early stages of experiment if compared wuith monotherapy.

Probl Cryobiol Cryomed 2019; 29(2):125–136.


Keywords


cryopreserved tissue; human placenta; calcium citrate; reparative osteogenesis; mandible fracture; bone tissue metabolism; rats

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DOI: https://doi.org/10.15407/cryo29.02.125

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