Temperature Dependence of SPEV Cell Membranes Permeability for Molecules of Cryoprotectants

Authors

  • N. A. Chernobay Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. V. Koschiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. Ye. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. F. Rozanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

permeability coefficients, activation energy, SPEV cells, volumometry

Abstract

Using the method of volumometry the permeability coefficients of SPEV cell membranes for molecules of ethylene glycol (EG), 1,2-butane diol (1,2-BD), glycerol, dimethyl sulfoxide (DMSO) and dimethyl formamide (DMFA) at 35, 20 and 5°С have been determined. The activation energy values of transport for molecules of EG, 1,2-BD and DMSO through the plasma membrane in temperature range of 35...5°C are close and approximately three times as higher than the activation energy of glycerol transport. The permeability coefficients at5°Cfor glycerol and 1,2-BD are almost the same, for DMSO and EG are approximately three times as higher, and the permeability coefficient for DMFA is four orders higher. The permeability for DMFA molecules at35°Cis comparable with permeability of molecules of water, and the permeability coefficients for glycerol are one order lower, than the ones for EG, 1,2- BD and DMSO.

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Published

2011-02-21

How to Cite

Chernobay, N. A., Kovalenko, I. F., Koschiy, S. V., Kovalenko, S. Y., & Rozanov, L. F. (2011). Temperature Dependence of SPEV Cell Membranes Permeability for Molecules of Cryoprotectants. Problems of Cryobiology and Cryomedicine, 21(1), 46–51. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/153

Issue

Vol. 21 No. 1 (2011): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 N. A. Chernobay, I. F. Kovalenko, S. V. Koschiy, S. Ye. Kovalenko, L. F. Rozanov

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