Impact of Lyophilisation on Integrity of Structural and Functional Characteristics of Human Cord Blood Leukoncentrate

Authors

  • Ganna K. Koval Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olena D. Lutsenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Igor G. Grisha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Larisa V. Sokil Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Mykola O. Bondarovych Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Maksim V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Kateryna Ye. Yampolskaya Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Lyudmila V. Ostankova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Anatoliy M. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo29.04.332

Keywords:

lyophilisation, human cord blood leukoconcentrate, colony forming units, hematopoietic stem cells, T-regulatory cells

Abstract

This paper presents the investigations on the impact of lyophilisation conditions on structural and functional characteristics of human cord blood leukoconcentrate (HCBL) such as: the number and viability, cytomorphological indices, content of hematopoietic stem (CD34+) and T-regulatory cells (CD4+CD25high), hematopoietic colony forming units in culture (CFUc). Four regimens, including two programs with and without 3.5% DMSO were used for hcbl cell lyophilisation. The programs differed in temperature value and duration wherein the primary drying proceeded. When using the first program, the cells were dried for 10 hrs (–28ºС), and 9 hrs (–18ºС) for the second one. The designed method for HCBL lyophilisation, suggested the use of the DMSO-free first program, ensured the cell preservation in a heterogeneous population of cord blood according to all the studied characteristics.

 

Probl Cryobiol Cryomed 2019; 29(4): 332-343

Author Biographies

Ganna K. Koval, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Olena D. Lutsenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Igor G. Grisha, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Larisa V. Sokil, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Mykola O. Bondarovych, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Maksim V. Ostankov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Kateryna Ye. Yampolskaya, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Lyudmila V. Ostankova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Anatoliy M. Goltsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

References

Akel S, Regan D, Wall D, et al. Current thawing and infusion practice of cryopreserved cord blood: the impact on graft quality, recipient safety, and transplantation outcomes. Transfusion. 2014; 54(11): 2997-3009. CrossRef

Ananyina AYe, Tsutsayeva AA, Balyberdina LM, et al. Lyophilisation of Streptomyces aureofaciens endospore culture. Problems of Cryobiology. 2008; 18(4): 404-6.

Castellano JM, Mosher KI, Abbey RJ, et al. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice. Nature. 2017; 544 (7651): 488-92. CrossRef

Dufresne J, Hoang T, Ajambo J, et al. Freeze-dried plasma proteins are stable at room temperature for at least 1 year. Clin Proteomics [Internet]. 2017 [cited 27.10.2017]; 14: 35. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5659006/ CrossRef

Frimel G., editor. [Immunological methods]. Moskow: Meditsina; 1987. 472 p. Russian.

Goltsev AM, Sokol LV, Stetsyshyn VG, Mosiychuk VV, Bondarovich MO, Grisha IG, Ostankov MV, Lutsenko OD, Ostankova LV, Chernyshenko LH inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of lyophilization of leukoconcentrate of cord blood]. Ukraine patent N 125846, 2018 May 25. Ukranian.

Goltsev AM, Taranik GC, Grisha IG, Sokil LV, Bondarovich MO, Ostankov MV, Lutsenko OD, Goltsev KA, Ostankova LV, inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of lyophilization of leukoconcentrate of cord blood]. Ukrainian patent 113006, 2017 Jan 10. Ukranian.

Goltsev AN, Volina VV, Ostankov MV, et al. Effect of cryopreservation on functional properties of human cord blood leukoconcentrate nucleated cells. Problems of Cryobiology. 2010; 20(1): 66-72.

Ikeda K, Ohto H, Okuyama Y, et al. Adverse events associated with infusion of hematopoietic stem cell products: a prospective and multicenter surveillance study. Transfus Med Rev. 2018. 32(3): 186-94. CrossRef

International standards for cord blood collection, banking, and release for administration. 6th ed. Omaha (NE): NetCord-FACT; 2016.

Knapp HF, Hammond CA, Hui T, et al Single-cell analysis identifies a CD33+ subset of human cord blood cells with high regenerative potential. Nat Cell Biol. 2018; 20(6): 710-20. CrossRef

Lebedinets VV, Ostankova LV, Dubrava TG, et al. [Use of cryopreserved cord blood to correct immune system in a model of ischemic stroke]. Medytsyna Sogodni i Zavtra. 2015; (4): 2-9. Russian.

Lecchi L, Giovanelli S, Gagliardi B, et al. An update on methods for cryopreservation and thawing of hemopoietic stem cells. Transfus Apher Sci. 2016; 54 (3): 324-36. CrossRef

Li J, Hua TC, Gu XL, Ding Y, et al. Morphology study of lyophilisation mononuclear cells of human cord blood. CryoLetters. 2005; 26(3): 193-200. PubMed

Lo Presti V, Nierkens S, Boelens JJ, van Til NP. Use of cord blood derived T-cells in cancer immunotherapy: milestones achieved and future perspectives. Expert Rev Hematol. 2018; 11(3): 209-18. CrossRef

Makashova OE, Babijchuk LO, Zubova OL, Zubov PM. Optimization of cryopreservation technique for human cord blood nucleated cells using combination of cryoprotectant DMSO and antioxidant N-acetyl-L-cysteine. Probl Cryobiol Cryomed. 2016; 26(4): 295-307. CrossRef

Maral S, Albayrak M, Pala C, et al. Dimethyl sulfoxide-induced tonic-clonic seizure and cardiac arrest during infusion of autologous peripheral blood stem cells. Cell Tissue Bank. 2018; 19(4): 831-2. CrossRef

Menshikov VV, editor. [Laboratory methods for research in clinics]. Moscow: Meditsina, 1987. p. 123-5. Russian.

Merkulov GA. [The course of histological-pathological technics]. Leningrad: Medgiz; 1961. 343 p. Russian.

Natan D, Nagler A, Arav A. Freeze-drying of mononuclear cells derived from umbilical cord blood followed by colony formation. Plos One [Internet]. 2009 [cited 21.04.209]; 4 (4): e5240. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0005240 CrossRef

Pushkar NS, Belous ÀÌ, Tsvetkov TsD [Theory and practice of cryogenic and sublimation preservation]. Kyiv: Naukova Dumka; 1984. 259 p. Russian.

Shereshkov S.I. [Culturing of hemopoietic cells on semisolid nutrient media]. Laboratornoe Delo. 1974; (3): 146-50. Russian.

Takanashi M, Selogie E, Reems JA, et al. Current practices for viability testing of cryopreserved cord blood products: an international survey by the cellular therapy team of the Biomedical Excellence for Safer Transfusion (BEST) Collaborative. Transfusion. 2018; 58(9): 2184-91. CrossRef

Tarannik AK, Ostankova LV, Grisha IG, et al. Frozen-dried human cord blood leukoconcentrate as correcting agent of immune status when treating atopic dermatitis (experimental study). Probl Cryobiol Cryomed. 2016; 26(2): 165. CrossRef

Varlamova TI, Kukunina TV, Zhmykhov AA. [Study of the influence of primary crystallization on formation of lyophilized product macrostructure during freezing of drug solutions]. Vestnik Sovremennykh Issledovaniy. 2017; 12(9): 6-11. Russian.

Wakayama S, Ito D, Kamada Y, et al. Tolerance of the freeze-dried mouse sperm nucleus to temperatures ranging from -196°C to 150°C. Sci Rep [Internet]. 2019 [cited 09.11.2019]; 9(1):5719. Available from: https://www.nature.com/articles/s41598-019-42062-8.pdf CrossRef

Xiao HH, Hua TC, Li J, et al. Freeze-drying of mononuclear cells and whole blood of human cord blood. CryoLetters. 2004; 25(2): 111-20. PubMed

Zacarías MF, Binetti A, Bockelmann W, et al. Safety, functional properties and technological performance in whey-based media of probiotic candidates from human breast milk. Int Microbiol. 2019; 22(2): 265-77. CrossRef

Published

2019-12-17

How to Cite

Koval, G. K., Lutsenko, O. D., Grisha, I. G., Sokil, L. V., Bondarovych, M. O., Ostankov, M. V., Yampolskaya, K. Y., Ostankova, L. V., & Goltsev, A. M. (2019). Impact of Lyophilisation on Integrity of Structural and Functional Characteristics of Human Cord Blood Leukoncentrate. Problems of Cryobiology and Cryomedicine, 29(4), 332-343. https://doi.org/10.15407/cryo29.04.332

Issue

Section

Theoretical and Experimental Cryobiology