Cryosurgical Method for Nervus Vagus Ablation in Experimental Studies
Keywords:cryoablation, nervus vagus, aorta, experiment, denervation, baroreceptors, endoscopy
Abstract: When studying the impact of nervous system on physiological and pathophysiological processes, a need arises to determine the contribution of each division of autonomic nervous system. For the nervus vagus ablation in cervical spine, chest and abdominal cavities the cryosurgical method has been designed to assess the effect of parasympathetic division on heart rate variability. There has been shown the possibility to implement the nervus vagus ablation, as well as the arteria carotis cryodenervation using cryosurgical technique in the ‘carotid’ triangle area of the neck, and thereby to ‘switch off’ the vascular intima-located baroreceptors from a regulatory link. The use of endoscopic device and cryosurgical technique for vascular denervation was established to enable the aortic arch baroreceptor ablation while preserving an anatomical integrity of the vessel. Since the isolation procedure for the nervus vagus abdominal branches in rat along the esophageal lateral edges is relatively accessible, it allows performing the cryodenervation of individual and both nerve branches along with esophagus.
Probl Cryobiol Cryomed 2020; 30(1): 090–098
Boyko VV, Khvorostov ED, Sushkov SV. [Сryovagatomy in patient with duodenal peptic ulcer]. The Journal of V.N. Karazin Kharkiv National University. Series Medicine. 2004; (614): 30-3. Russian.
Bozkurt A, Tholl S, Wehner S. et al. Evaluation of functional nerve recovery with Visual-SSI - A novel computerized approach for the assessment of the static sciatic index (SSI). J Neurosci Meth. 2008; 170(1): 117-22. CrossRef
Chizh NA. Parameters of spectral analysis of heart rate variability in rats. Probl Cryobiol Cryomed. 2015; 25(3): 235-45. CrossRef
Chizh N.A., Byzov D.V., Antonenko Ye.A. et al. [Endosocopic device for surgeries in experimental animals]. Fiziol Zh. 2017; 63(2): 86-94. Ukraine. CrossRef
Chizh NA, Marchenko LN, Belochkina IV, et al. [Ultrastructure of hepatic artery after cryodenervation]. Ukr Morfol Almanakh. 2008; 6(3): 84-7. Russian
Chizh MO, Belochkina IV, inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of denervation of the baroreceptor of the aortic arch in experiment]. Ukraine patent N 135663, 2019 July 10. Ukrainian.
Inoue H, Zipes DP. Changes in atrial and ventricular refractoriness and in atrioventricular nodal conduction produced by combinations of vagal and sympathetic stimulation that result in a constant spontaneous sinus cycle length. Circulation research. 1987; 60(6):942-51. CrossRef
Grischenko VI, Sandomirskiy BP. [Practical cryomedicine]. Kyiv: Zdorov'ya; 1987. 246 p. Russian.
Kondakov IK, Yakovtsova AF, Gavrish AS. [Ways to estimate the morphofunctional state of vascular endothelium. Methodical recommendations.] Kharkiv, Kyiv; 2000. 19 p. Russian.
Miroshnikova ME, Chumasov EI. [Regeneration of the sciatic nerve of the rat after its various experimental injuries]. Archive of anatomy, histology and embryology. 1988; 95(10): 30-5. Russian
Nozdrachev AD, Polyakov EL. [Anatomy of rat (Laboratory animals)]. Saint Petersburg: Lan', 2001. 464 p. Russian.
Schmidt RF, Thews G. Human physiology. Berlin: Springer-Verlag; 1989. 825 p. CrossRef
Sinelnikov RD. [Atlas of human anatomy]. Moscov: Meditsina; 1987. Vol. 2. 558 p. Russian
Syrovnev VA, Lebedev DS, Mikhaylov EN. [Vagus nerve stimulation in cardiology]. Translational Medicine. 2017; 4(2): 6-16. Russian. CrossRef
Taha M. Effects of denervation on baroreceptor and chemoreceptor endings in the aorta and pulmonary trunk of the domestic fowl (Gallus gallus domesticus). J Anat. 1987; 150:49-60.
Verkin BI, Medvedev ЕМ, Murinec-Markevich BM, Utemov TV. inventors; Institute for Low Temperature Physics and Engineering of the National Academy of Sciences of Ukraine, assignee. [Device for local tissue cooling]. USSR C.A. (certificate of authorship) № 528090. 1976 Sept.15. Russian.
How to Cite
Copyright (c) 2020 Mykola Chyzh, Igor Belozorov , Dmytro Aschabov , Dmytro Komyshanchenko , Vyacheslav Globa , Larysa Marchenko , Yulia Chyzh
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).