Ultrastructural and functional characteristics of human spermatozoa after cryopreservation by vitrification

Authors

  • Olena Pavlovych Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Hanna Hapon Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Taisiia Yurchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Mykola Repin Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Larysa Marchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Tetiana Govorukha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Maryna Petrushko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo30.01.024

Keywords:

spermatozoa, cryopreservation, vitrification, asthenozoospermia, polyvinylpyrrolidone

Abstract

Low temperature preservation of spermatozoa is widely applied in infertility treatment when using the assisted reproductive technology (ART). There are the standard methods for ejaculated spermatozoa cryopreservation in normozoospermia, but their application for ejaculate-derived spermatozoa, having spermatogenesis defects, is impossible. Therefore, of high priority are to design the cryopreservation methods and assess morphofunctional and ultrastructural characteristics of spermatozoa after cryopreservation. Here, we have evaluated the impact of cryopreservation by vitrification using non-penetrating cryoprotectants of polyvinylpyrrolidone (PVP) and sucrose on morphofunctional and ultrastructural state of human spermatozoa in pathospermia. In (22.3 ± 3.4),(26.8 ± 4.2), (18.6 ± 2.1)% of spermatozoa after vitrification with 0.25M sucrose; 10% PVP and a mixture of 0.25 M sucrose, 10% PVP, 10% HSA, respectively, we have observed the appearance of vacuoles in nuclear chromatin of spermatozoa. For freshly isolated spermatozoa this index was (16.6 ± 1.4)%. In the studied groups, the ultrastructural characteristics of acrosome, axoneme, periaxonemal structures, and spermatozoa flagella did not significantly differ from the control samples. The cryopreservation of human spermatozoa by vitrification using sucrose and PVP solutions was shown to preserve their viability and ultrastructural integrity and to be promising in ART.

Probl Cryobiol Cryomed 2020; 30(1): 24-33

Author Biographies

Olena Pavlovych , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproductive System

Hanna Hapon , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproductive System

Taisiia Yurchuk , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproductive System

Mykola Repin , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryomorphology

Larysa Marchenko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryomorphology

Tetiana Govorukha , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Laboratory of Cryomorphology

Maryna Petrushko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproductive System

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Published

2020-03-28

How to Cite

Pavlovych , O., Hapon , H., Yurchuk , T., Repin , M., Marchenko , L., Govorukha , T., & Petrushko , M. (2020). Ultrastructural and functional characteristics of human spermatozoa after cryopreservation by vitrification. Problems of Cryobiology and Cryomedicine, 30(1), 24–33. https://doi.org/10.15407/cryo30.01.024

Issue

Section

Theoretical and Experimental Cryobiology