Choice of Vitrification Mode for Macroporous Matrices Seeded with Mesenchymal Stromal Cells

Authors

  • Natalia Trufanova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Igor Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oleksandr Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo30.01.077

Keywords:

tissue engineered constructs, macroporous matriсes, vitrification, devitrification, cracking, mesenchymal stromal cells, viability

Abstract

Abstract: The composition of the solution of cryoprotectants and vitrifi cation conditions (saturation methods, removal of cryo-protectants, cooling-warming modes) of tissue engineered constructs based on macroporous matrices (MPM) and mesenchymal stromal cells (MSCs), preventing the development of crystallization and cracking of a glassy phase, were determined and the infl uence of the developed conditions on cell viability and MPM structure was studied. Crystallization and cracking were investigated during MPM cooling and warming in cryoprotectant solutions in cryovials. The viability of MSCs in suspension was determined by trypan blue staining, and that as a part of MPM was examined by ethidium bromide and fl uorescein diacetate staining. The MSCs metabolic activity was evaluated with the MTT test. The selected conditions were shown to provide a high viability of MSCs after vitrifi cation in the form of a suspension, but required an improvement to preserve the cells seeded in macroporous matrices.

 

Probl Cryobiol Cryomed 2020; 30(1): 077–089

Author Biographies

Natalia Trufanova , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry

Igor Kovalenko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Low Temperature Preservation

Oleksandr Petrenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiochemistry

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Published

2020-03-28

How to Cite

Trufanova , N., Kovalenko , I., & Petrenko, O. (2020). Choice of Vitrification Mode for Macroporous Matrices Seeded with Mesenchymal Stromal Cells. Problems of Cryobiology and Cryomedicine, 30(1), 77-89. https://doi.org/10.15407/cryo30.01.077

Issue

Section

Cryopreservation of Biological Resources