Impact of combined effect of penetrating and non-penetrating medium components on post-hypertonic lysis development in human erythrocytes

Authors

  • Olena O. Chabanenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Natalia V. Orlova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Natalia M. Shpakova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo30.03.236

Keywords:

post-hypertonic shock, post-hypertonic lysis, combined media, glycerol, erythrocytes

Abstract

Abstract: In this research, a post-hypertonic lysis of human erythrocytes has been studied by varying the medium composition at dehydration stage. The osmolality of combined media, containing diff erent concentrations of glycerol and NaCl was ~ 2370 mOsm/L. The level of post-hypertonic lysis of erythrocytes was shown as determined not by the total osmolality of dehydration medium, but glycerol concentration within the combined media. It was found that the nature of post-hypertonic lysis development of erythrocytes if using glycerol within the combined media at dehydration stage did not depend on preliminary cell saturation with cryoprotectant. A comparative study of post-hypertonic shock of erythrocytes at 37 and 0°C showed that in the latter case the cell hemolysis deve-loped if using glycerol in lower concentration (10%) within the combined media (at 0°C the level of erythrocyte post-hypertonic lysis was 2.7 times higher). The observed eff ect may be due to a signifi cant diff erence in the rates of transport for water and cryoprotectant through erythrocyte membrane at low temperatures.

Probl Cryobiol Cryomed 2020; 30(3): 236–246

 

Author Biographies

Olena O. Chabanenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Natalia V. Orlova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Natalia M. Shpakova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

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Published

2020-09-23

How to Cite

Chabanenko, O., Orlova, N., & Shpakova, N. (2020). Impact of combined effect of penetrating and non-penetrating medium components on post-hypertonic lysis development in human erythrocytes. Problems of Cryobiology and Cryomedicine, 30(3), 236-246. https://doi.org/10.15407/cryo30.03.236

Issue

Section

Theoretical and Experimental Cryobiology