Viability of Biﬁdobacterium biﬁdum 1 under hypothermia, single and repeated freeze-thaw cycles.
Keywords:biﬁdobacteria, viability, freeze-thaw, thermal cycling, daily biomass growth, bioﬁlm formation
Abstract: The viability of bacteria of the Biﬁdobacterium biﬁdum 1 probiotic strain under hypothermia, single and repeated freeze-thaw cycles (thermal cycling) was studied. Samples of biﬁdobacterial suspensions were frozen immediately after isolation or after daily hypothermic storage in three ways to the ﬁnal temperature of either (–23 ± 1) or (–196 ± 1)ºС. After slow freezing of the samples down to (–23 ± 1) ºС, bigger quantitative losses of biﬁdobacteria were observed if compared with those after rapid freezing by a direct immersion into liquid nitrogen. Storage of the samples under hypothermia and a single freeze-thaw was accompanied with a strong inhibition of the daily growth of biﬁdobacteria biomass and an increased formation of bioﬁlms. Ten-fold thermal cycling in the most unfavorable way for survival did not lead to the death of all cells in suspensions. Up to 35% of biﬁdobacteria remained viable. Indices of the biﬁdobacteria ability to enhance biomass remained at the level of 35%, and the ability to form bioﬁlm was kept at the level of 43.7–65.5% of the corresponding indices for freshly isolated cells.
Probl Cryobiol Cryomed 2020; 30(3): 247–255
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Copyright (c) 2020 Oksana V. Knysh, Oleksandr V. Pakhomov, Antonina М. Kompaniets, Valentina P. Polianska, Svitlana V. Zachepylo
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