Application of Vacuum Infiltration in Cryopreservation of Isolated Grape Buds
Keywords:grape buds, cryopreservation, vacuum infiltration, vitrification solution, phase transitions, calorimetry
Preserving the gene pool of grapes, referred to the vegetatively propagated plants is a complicated task, which can be also solved by cryopreservation of the buds. To saturate such bulk and heterogeneous samples with cryoprotectants the novel methods are required. The effectiveness of vacuum infiltration and 60 min standard passive saturation (soaking) of isolated grape buds of the Russian Concord variety with a cryoprotective solution PVS 2 were compared in this research. To saturate by vacuum infiltration the buds were incubated in cryoprotective solution for 15 min at 40 kPa, afterwards, the pressure was gradually increased to atmospheric level. The efficiency of bud saturation was evaluated with low-temperature differential scanning calorimetry by changing the enthalpies and temperatures of phase transitions as well as the intensity of heat capacity jump at glass transition. The use of vacuum in the saturation of isolated grape buds were found to lead to a strong rise in cryoprotectant concentration in them and a significant decrease in the amount of free water crystallized during cooling compared to passive soaking in a PVS 2 vitrification solution.
Probl Cryobiol Cryomed 2021; 31(1): 051–057
Funnekotter B, Whiteley SE, Turner SR, et al. Evaluation of the new vacuum infiltration vitrification (viv) cryopreservation technique for native Australian plant shoot tips. CryoLetters. 2015; 36(2): 104−13. PubMed
Kalaiselvi R, Rajasekar MA, Sankara GS. Cryopreservation of plant materials - a review. Int J Chem Stud. 2017; 5(5): 560−4.
Kiryanov KV. [Calorimetric research methods]. Nizhny Novgorod: Educational and scientific centre; 2007. 78 p. Russian.
Nadarajan J, Pritchard HW. Biophysical characteristics of successful oilseed embryo cryoprotection and cryopreservation using vacuum infiltration vitrification: аn innovation in plant cell preservation. PLOS ONE. [Internet]. 2014 May 1 [cited 2020 May 29]: 9(5): e96169. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0096169 CrossRef
Ogawa Y, Sakurai N, Oikawa A, et al. High-throughput cryopreservation of plant cell cultures for functional genomics. Plant Cell Physiol. 2012; 53(5): 943-52. CrossRef
Prystalov AI, Bondar IN, Polulyakh AA, et al. [Viticulture of Sloboda Ukraine: history, problems, prospects for establishing and preservation of collections]. The Journal of V.N.Karazin Kharkiv National University. Series: biology. 2014; 1100(20): 53−60. Russian.
Prystalov AI, Rozanov LF, Kuleshova LG, inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Device for vacuum infiltration of cuttings of fruit and berry crops]. Patent of Ukraine № 121556, 2017 Dec 11. Ukrainian.
Prystalov AI, Shevchenko NO, Zelenjanska NM, Kuleshova LG, inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of washing cuttings of fruit and berry cultures from cryoprotectants]. Patent of Ukraine № 136543, 2019 Aug 27. Ukrainian.
Ruzic D, Vujović T, Cerović R. Cryopreservation of cherry rootstock Gisela 5 (Prunus cerasus x Prunus canescens) shoot tips by droplet-vitrification technique. Journal of Horticultural Research. 2013; 21(2): 79−85. CrossRef
Sakai A, Hirai D, Niino T. Development of PVS-based vitrification protocol. In: Reed B, editor. Plant cryopreservation: a practical guide: New York: Springer; 2008. p. 33-57. CrossRef
Shevchenko NO, Prystalov AI, Stribul TF, inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Laboratory device for vacuum infiltration of cuttings of fruit and berry crops]. Patent of Ukraine № 85644, 2013 Nov 25. Ukrainian.
Tanaka D, Niino T, Uemura M. Cryopreservation of plant genetic resources. Adv Exp Med Biol. 2018; 1081: 355-69. CrossRef
Uragami A, Sakai A, Nagai M, et al. Survival of cultured cells and somatic embryos of Asparagus officinalis cryopreserved by vitrification. Plant Cell Reports. 1989; 8(7): 418-21. CrossRef
Volis S. How to conserve threatened Chinese plant species with extremely small populations? Plant Diversity. 2016; 38(1): 45-52. CrossRef
Volk GM, Walters C. Plant vitrification solution 2 lowers water content and alters freezing behaviour in shoot tips during cryoprotection. Cryobiology. 2006; 52: 48-61. CrossRef
Zamecnik J, Faltus M, Bilavcik A, Kotkova R. Comparison of cryopreservation methods of vegetatively propagated crops based on thermal analysis. In: Igor Katkov, editor. Current Frontiers in Cryopreservation; [Internet]. 2012 Mar 14; [cited 2020 May 29]: 333-58. Available from: https://www.intechopen.com/books/current-frontiers-in-cryopreservation/comparison-of-cryopreservation-methods-of-vegetatively-propagated-crops-based-on-thermal-analysis CrossRef
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