Experimental Cryoablation of Thyroid Gland with Propilthiouracyl-Induced Diffuse Hyperplasiae

Authors

  • Kostiantyn O. Pobielienskyi Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oleksandr V. Pakhomov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; V.N. Karazin Kharkiv National University, Kharkiv https://orcid.org/0000-0002-7494-654X
  • Tetyana M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Liliia A. Pobielienska Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; V.N. Karazin Kharkiv National University, Kharkiv
  • Ievgen I. Legach Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Galyna A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; V.N. Karazin Kharkiv National University, Kharkiv https://orcid.org/0000-0002-4188-9286

DOI:

https://doi.org/10.15407/cryo31.02.168

Keywords:

thyroid gland, cryoablation, propylthiouracil, diffuse hyperplasia, rats

Abstract

Cryoablation under endoscopic control is considered to be a promising approach in therapy of benign nodules of thyroid gland (TG). However, pathologically altered TG tissue differs in thermal conductivity and heat capacity from normal one, therefore the model experiments in animals are necessary to determine the cryoablation parameters. In this research, the changes of temperature during cryoablation of experimental rat TG under normal conditions and the one with propylthiouracil (PTU)-induced diffuse hyperplasia (DH) were comparatively assessed. TG was cryo-ablated in rats, previously received a 0.1% PTU solution within 90 days, using a copper cryoprobe, cooled to liquid nitrogen temperature. The process was controlled using thermocouples placed at different distances from the iceball. Differences between thermograms of intact TG tissue and the samples with PTU-induced DH were established. To achieve the destruction effect of TG with DH to a depth of more than 1 mm, the need of implementing two freeze-thaw cycles with 120-second cryoprobe exposure was proven.

Probl Cryobiol Cryomed 2021; 31(2): 168–179

 

Author Biographies

Kostiantyn O. Pobielienskyi , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryoendocrinology

Oleksandr V. Pakhomov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; V.N. Karazin Kharkiv National University, Kharkiv

Department of Cryoendocrinology

Tetyana M. Gurina , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryomicrobiology

Liliia A. Pobielienska , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; V.N. Karazin Kharkiv National University, Kharkiv

Department of Cryoendocrinology

Ievgen I. Legach , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryoendocrinology

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Published

2021-07-15

How to Cite

Pobielienskyi, K., Pakhomov, O., Gurina, T., Pobielienska, L., Legach, I., & Bozhok, G. . (2021). Experimental Cryoablation of Thyroid Gland with Propilthiouracyl-Induced Diffuse Hyperplasiae. Problems of Cryobiology and Cryomedicine, 31(2), 168–179. https://doi.org/10.15407/cryo31.02.168

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Section

Cryomedicine, Clinical and Experimental Transplantology