Lyophilized Human Cord Blood Leukoconcentrate Affects Immune System of Rats with Ischemic Stroke
DOI:
https://doi.org/10.15407/cryo32.04.277Keywords:
lyophilization, cryopreservation, human cord blood leukoconcentrate, ischemic stroke, immune system, circulating immune complexesAbstract
Cerebrovascular pathology, especially acute cerebrovascular accident is among the most complicated issues in medicine. Therefore, the search for efficient ways to treat neurodegenerative diseases has still remained topical. In experimental model of ischemic stroke (IS), the impact of lyophilized and cryopreserved human cord blood leukoconcentrate (lHCBL and cHCBL, respectively) on immune system humoral and cell components has been comparatively studied. The ischemic stroke was simulated in 6-monthold Wistar rats by occlusion of the middle cerebral artery. The lHCBL and cHCBL were administered intraperitoneally at a dose of 0.5 ml of 5 × 106 cells in 6 hrs after surgery (occlusion). In blood serum, the populations and subpopulations of lymphocytes (CD3+, CD4+, CD8+, CD16+, CD4+CD25+), the content of total immunoglobulins and circulating immune complexes were determined. The experimental model of IS showed both lyophilization and cryopreservation of HCBL to ensure the preservation of immunomodulatory potential of cells, that was confirmed in vivo. An equivalent corrective effect of lHCBL and cHCBL on indices of immune system cell and humoral components during IS development has been established. The inclusion of lHCBL, as well as cHCBL and native HCBL in basic therapy of IS promoted a more rapid recovery of the immune system indices in animals.
Probl Cryobiol Cryomed 2022; 32(4):277–291
References
Baccetto LS, Lehmann C. Microcirculatory changes in experimental models of stroke and CNS-injury induced immunodepression. [Internet] Int J Mol Sci. 2019 Oct 19 [cited 2020 June 15]; 20(20): E5184. Available from: https://www.mdpi.com/1422-0067/20/20/5184 CrossRef
Belderbos ME, Levy O, Meyaard L, Bont L. Plasma-mediated immune suppression: a neonatal perspective. Pediatr Allergy Immunol. 2013; 24(2): 102-13. CrossRef
Dhir N, Medhi B, Prakash A, et al. Pre-clinical to clinical translational failures and current status of clinical trials in stroke therapy: a brief review. Curr Neuropharmacol. [Internet]. 2020 Jan 14 [cited 2020 June 15]; 18(1): 596-612. Available from: http://www.eurekaselect.com/178306/article CrossRef
Goltsev AN, Volina VV, Ostankov MV, et al. Effect of cryopreservation on functional properties of human cord blood leukoconcentrate nucleated cells. Problems of Cryobiology. 2010; 20 (1): 66-72. Full Text
Goltsev AN, Kalinichenko TA. Human umbilical cord blood as a source of hemopoietic cells for clinical application. Part II. Immunologic charactieristics. Problems of Cryobiology. 1998; (1): 3-21.
Goltsev AM, Mosiychuk VV, Goltsev KA, et al. inventors; Institute for Problems of Cryobiology and Cryomedicine, assignee. [Method of lyophilization of leukoconcentrate of cord blood]. Ukrainian patent N 117780U, 2017 Jule 10. Ukrainian.
Goltsev KA, Ostankov MV, Kozhina OYu, et al. Application of cryopreserved cord blood in combined therapy of acute purulent peritonitis. Scientific Bulletins. Medicine. Pharmacy. 2013; (18): 114-9.
Harris DT, Badowski M, Ahmad N, Gaballa MA. The potential of cord blood stem cells for use in regenerative medicine. Expert Opin Biol Ther. 2007; 7(9): 1311-22. CrossRef
Javidi E, Magnus T. Autoimmunity after ischemic stroke and brain injury. Front Immunol. [Internet]. 2019 Apr 2 [cited 2020 June 15]; 10: 686. Available from: https://www.frontiersin.org/articles/10.3389/fi mmu.2019.00686/full CrossRef
Khamitova AR, Rakhimkulov AS, Borisova NA, Khuzhin IU. [Analysis of the incidence of cerebrovascular diseases in Salavatsky and Karaidelsky district of Bashkortostan]. Bashkortostan Medical Journal. 2014; 9(6): 89-92. Russian.
Koval AK, Lutsenko ED, Grisha IG, et al. Impact of lyophilization on integrity of structural and functional characteristics of human cord blood leukoncentrate. Probl Cryobiol Cryomed. 2019; 29 (4): 333-43. CrossRef
Koval AK, Ostankov MV, Bondarovich NA, et al. Effect of cryopreserved cord blood on functional state of dendritic cells of rat skin with atopic dermatitis. Probl Cryobiol Cryomed. 2018; 28 (2): 159. CrossRef
Lebedinets VV, Ostankova LV, Bondarovych MO, et al. Lyophilized human cord blood leucoconcentrate in complex treatment of rat brain ischemia. Probl Cryobiol Cryomed. 2022; 32 (1): 44-57. CrossRef
Menshikov VV [Laboratory methods for research in clinics]. Moscow: Meditsina, 1987. P. 123-5. Russian.
Nian K, Harding IC, Herman IM, Ebong EE. Blood-brain barrier damage in ischemic stroke and its regulation by endothelial mechanotransduction. Front Physiol. 2020 Dec 22 [cited 2020 June 15]; 11: 605398. Available from: https://www.frontiersin.org/articles/10.3389/fphys.2020.605398/full CrossRef
Ovsepyan LM, Kazaryan GS, Zanginyan AV, Zakharyan GV. [Аnalysis of the expression level of T cell genes and oxidative processes in stroke]. Meditsinskaya Immunologiya. 2019; 21 (2): 251-6. Russian. CrossRef
Paster EU, Gadfly BB, Pozur VK, Vikhot NE. [Immunology laboratory course]. Кyiv: Vyshcha shkola; 1989. 304 p. Russian.
Sapon N, Nikiforova A. [Correlation between access to health care and stroke mortality]. Ukrainian Neurosurgical Journal, 2016, (2): 54-62. Russian. CrossRef
Serebrennikova SN, Seminskу IZh, Semenov NV, Guzovskaya EV. [Interleukin-1, interleukin-10 in regulation of inflammatory process]. Siberian Medical Journal. 2012; (8): 5-7. Russian.
Tarkowski E, Naver H, Wallin BG, et al. Lateralization of T-lymphocyte responses in patients with stroke. Effect of sympathetic dysfunction? Stroke. 1995; 26(1): 57-62. CrossRef
Tsutsaieva AO, Hryschenko VI, Kudokotseva OV, et al. inventors; Institute for problems of Cryobiology and Cryomedicine, assignee. [Method for cryopreservation of hemopoietic cells of cord blood]. Ukraine patent N 31847А, 2000 Dec. 15. Ukrainian.
Tsygan NV, Trashkov AP, Litvinenko IV, et al. Autoimmunity in acute ischemic stroke and the role of blood-brain barrier: the dark side or the light one? Front Med. 2019 Aug; [cited 2020 June 15]; 13(4): 420-6. Available from: https://journal.hep.com.cn/fmd/EN/10.1007/s11684-019-0688-6 CrossRef
Yoo J, Kim HS, Seo JJ, et al. Therapeutic effects of umbilical cord blood plasma in a rat model of acute ischemic stroke. Oncotarget. 2016; 7(48): 79131-40. CrossRef
Zapadnyuk IP, Zapadnyuk VI, Zakharia EA, Zapadnyuk BV. [Laboratory animals]. Kiev: Vyscha shkola, 1983. 252 p. Russian.
Zhao J, Chen N, Shen N, et al. Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury. Neural Regen Res. 2012; 7(10):741-8. CrossRef
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