Antibiotic Sensitivity of Escherichia Coli Museum Strains After Long-Term Storage in a Lyophilized State at Moderately Low Temperatures




lyophilized museum strains, moderately low temperatures, Escherichia coli, antibiotic resistance


Sensitivity of microbial cultures to antibiotics was determined using the disco-diffusion method, broth serial dilution and agar serial dilution. Restored viability and detection of antibiotic resistance in the museum Escherichia coli strains stored for a lasting time in a lyophilized state indicates that freeze-drying in combination with moderately low temperatures for subsequent long-term storage is an effective method of maintaining a collection of microorganism strains in a functionally stable state, including preservation of their antibiotic resistance. It was found that one of the 21 studied strains was resistant to ampicillin, ampicillin-sulbactam, cephalosporins of the I–III generations, two strains were resistant to chloramphenicol, and four strains were insensitive to tetracycline. Detection of the resistance to cephalosporins and protected penicillins in E. coli strain isolated in 1963, long before the discovery and use of these antibiotics in clinical practice, confirms that antibiotic resistance is a phenomenon associated rather with the general mechanisms of adaptation of pathogens to adverse external environmental factors than with the widespread use of antimicrobial drugs.


Probl Cryobiol Cryomed 2023; 33(2):115121

Author Biography

Tetiana M. Gurina , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryomicrobiology


Antomonov MYu. [Mathematical processing and analysis of biomedical data]. Kyiv: Medinform 2017: 578p. Russian.

Blanquart F. Evolutionary epidemiology models to predict the dynamics of antibiotic resistance. Evol Appl. 2019; 12(3): 365-83. CrossRef

Bodey GP, Terrell LM. In vitro activity of carbenicillin against gram-negative bacilli. J Bacteriol. 1968; 95(5): 1587-90. CrossRef

Brumfitt W, Percival A, Leigh D. Clinical and laboratory studies with carbenicillin. A new penicillin active against Pseudomonas pyocyanea. Lancet. 1967; 289(7503): 1289-93. CrossRef

Centers for disease control and prevention. Antibiotic resistance threats in the United States. 2013. [Internet]. 2013 April 23 [cited 2022 Oct 20]. Available from:

Fitchett JR. Antimicrobial resistance: opportunity for Europe to establish global leadership. Lancet Infect Dis. 2016; 16 (4):388-9. CrossRef

Jones RN, Fuchs PC, Gerlach EH, et al. Piperacillin and carbenicillin; a collaborative in vitro comparison against 10,838 clinical bacterial Isolates. Cleveland Clin Quart. 1979;46(1): 49-55. CrossRef

Maloy SR, Nunn WD. Selection for loss of tetracycline resistance by Escherichia coli. J Bacteriol. 1981; 145(2): 1110-1. CrossRef

Neu HC, Swarz H. Printed in Great Britain 301 resistance of Escherichia coli and Salmonella typhimurium to carbenicillin. J Gen Microbiol. 1969; 58(3): 301-5.


[On the approval of methodological guidelines: Determination of microorganisms' sensitivity to antibacterial drugs: Order of the Ministry of Health of Ukraine No 167], 2007 Apr 05. Kyiv: Ministry of Health of Ukraine, 2007. - 51p. Ukrainian.

Podolsky SH. The evolving response to antibiotic resistance (1945-2018). Palgrave Commun [Internet]. 2018 Oct 23 [cited 2023 Oct 17]; 4(1): 124. Available from: CrossRef

Ramey A, Ahlstrom C. Antibiotic resistant bacteria in wildlife: perspectives on trends, acquisition and dissemination, data gaps, and future directions. J Wildl Dis. 2020; 56(1): 1-15. CrossRef

Romanko MY, Ushkalov VO, Paliy AP, et al. Physiological activity of Salmonella spp. bacteria after lyophilization and rehydration. Probl Cryobiol Cryomed. 2022; 32(2): 158-163. CrossRef

Salmanov AG. [Resistance to antibiotics and biocides]. Int J Antibiotics and Probiotics. 2017; 1(2): 92-125. Ukrainian. CrossRef

Santiago-Rodriguez T, Fornaciari G, Luciani S, et al. Gut microbiome and putative resistome of inca and italian nobility mummies. Genes [Internet]. 2017 Nov 7 [cited 2022 Oct 19]; 8(11): 310. Available from: CrossRef

Taylor J, Hafner M, Yerushalmi E. Estimating the economic costs of antimicrobial resistance: Model and Results. Cambridge: RAND Corporation; 2014. 87p.

The European Committee on Antimicrobial Susceptibility. Routine and extended internal quality control for MIC determination and disk diffusion as recommended by EUCAST. Version 10.0. [Internet]. 2020 Jan 1 [cited 2022 Oct 13]. Available from:




How to Cite

Yagnuk , Y., Gurina, T., Peretyatko, O., Sklyar, N., Yagnuk А., & Kalinichenko, S. (2023). Antibiotic Sensitivity of Escherichia Coli Museum Strains After Long-Term Storage in a Lyophilized State at Moderately Low Temperatures. Problems of Cryobiology and Cryomedicine, 33(2), 115–121.



Theoretical and Experimental Cryobiology