Approaches to Creation of Cryoprotective Media for Cryopreservation of Avian Sperm
Keywords:avian sperm, cryopreservation, cryoprotective medium, cryoprotectants, cytotoxicity, diols, amides
A review of the literature and our own studies of the composition and physico-chemical properties of the cryoprotective media during cryopreservation of avian sperm is presented. Basic principle of forming media from components that perform specific functions in a cycle of freeze-thawing is proposed. We discuss the positive role of inclusion in the composition of the medium of protein supplements instead of lipids (egg yolk). We consider the prospects of complex compositions of cryoprotectants on the basis of diols and amides in combination with non-invasive water-soluble polymers. There are proposed rules of making a mixture of cryoprotectants based on the reduction of their cytotoxicity, the rate and mechanisms of their permeability through the membrane of spermatozoa, the impact on the processes of extra- and intracellular crystallization of osmotically active water.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).