Approaches to Creation of Cryoprotective Media for Cryopreservation of Avian Sperm

Authors

  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. N. Martynyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

avian sperm, cryopreservation, cryoprotective medium, cryoprotectants, cytotoxicity, diols, amides

Abstract

A review of the literature and our own studies of the composition and physico-chemical properties of the cryoprotective media during cryopreservation of avian sperm is presented. Basic principle of forming media from components that perform specific functions in a cycle of freeze-thawing is proposed. We discuss the positive role of inclusion in the composition of the medium of protein supplements instead of lipids (egg yolk). We consider the prospects of complex compositions of cryoprotectants on the basis of diols and amides in combination with non-invasive water-soluble polymers. There are proposed rules of making a mixture of cryoprotectants based on the reduction of their cytotoxicity, the rate and mechanisms of their permeability through the membrane of spermatozoa, the impact on the processes of extra- and intracellular crystallization of osmotically active water.

Published

2010-06-07

How to Cite

Linnik, T. P., & Martynyuk, I. N. (2010). Approaches to Creation of Cryoprotective Media for Cryopreservation of Avian Sperm. Problems of Cryobiology and Cryomedicine, 20(2), 109-122. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/200

Issue

Section

Theoretical and Experimental Cryobiology