Conditions for Loach (Misgurnus fossilis) Embryo Vitrification in Cryoprotective Media

  • K. B. Mikson Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. F. Kopeika Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Linnik Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
Keywords: embryos, mud loach, Misgurnus fossilis, cryoprotectant medium, vitrification

Abstract

Resistance of loach (Misgurnus fossilis) embryos to the effect of 5 cryoprotective media was investigated and the conditions of their vitrification were determined. The obtained experimental data demonstrated, that at a developmental stage, corresponding to the heart beating onset, the highest number of vitrified embryos (up to 100%) was observed during 30 min incubation within a cryoprotective medium, consisting of 30% sucrose, 10% ethylene glycol, 3% polyethylene oxide–1500 and 20% 1,2-propane diol. Vitrification temperature of loach embryos at this developmental stage was within –80...–176°C range.

Published
2009-07-06
How to Cite
Mikson, K. B., Kopeika, E. F., & Linnik, T. P. (2009). Conditions for Loach (Misgurnus fossilis) Embryo Vitrification in Cryoprotective Media. Problems of Cryobiology and Cryomedicine, 19(2), 154-162. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/270
Section
Theoretical and Experimental Cryobiology