Effect of Dimethyl Sulfoxide and Diethyl Sulfoxide on Thermal Denaturation of Human Serum Albumin

Authors

  • K. R. Grigoryan Yerevan State University, Yerevan
  • S. A. Markarian Yerevan State University, Yerevan
  • M. G. Aznauryan Yerevan State University, Yerevan

Keywords:

albumin, thermal denaturation, electron spectroscopy, fluorescent spectroscopy, dimethyl sulfoxide, diethyl sulfoxide

Abstract

The effect of dimethyl sulfoxide (DMSO) and diethyl sulfoxide (DESO) on thermal (heat and cold) denaturation of human serum albumin within temperature range from –20 to90°Cwas investigated by electron and fluorescent spectroscopy. It has been established that at heat induced denaturation in the presence of DESO an acid-neutral transition was observed at 47.61°C. It was shown that at cold induced denaturation the direct hydrophobic interactions of sulphoxide molecules with protein surface result in the formation of stable “ice†layer, providing the protein protection from irreversible structure changes.

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Published

2009-05-25

How to Cite

Grigoryan, K. R., Markarian, S. A., & Aznauryan, M. G. (2009). Effect of Dimethyl Sulfoxide and Diethyl Sulfoxide on Thermal Denaturation of Human Serum Albumin. Problems of Cryobiology and Cryomedicine, 19(1), 3–10. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/280

Issue

Vol. 19 No. 1 (2009): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 K. R. Grigoryan, S. A. Markarian, M. G. Aznauryan

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