Temperature Effect on Membrane Permeability Coefficients of Yeast-Like Fungi Saccharomyces cerevisiae for Water and Cryoprotectants

Authors

  • A. V. Sakun Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. V. Maruschenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. Yu. Sirenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. V. Davydova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. I. Gordiyenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

Saccharomyces cerevisiae, permeability, cryoprotectants, activation energy

Abstract

The biophysical parameters of yeast cell Saccharomyces cerevisiae, osmotically inactive volume, filtration coefficients in the cryoprotectant containing media and permeability coefficients for the cryoprotectants glycerol, 1,2-propane diol, dimethyl sulfoxide, were determined at the temperatures of 25 and 10°C. For determination of the parameters the improved equations of mass transfer via cell membranes were used. The equations are based on the approach of linear thermodynamics of irreversible processes. The values of activation energy of water and cryoprotectants molecules penetration via cell membranes were calculated.

Published

2009-05-25

How to Cite

Sakun, A. V., Maruschenko, V. V., Kovalenko, I. F., Sirenko, A. Y., Vysekantsev, I. P., Davydova, E. V., & Gordiyenko, O. I. (2009). Temperature Effect on Membrane Permeability Coefficients of Yeast-Like Fungi Saccharomyces cerevisiae for Water and Cryoprotectants. Problems of Cryobiology and Cryomedicine, 19(1), 41-48. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/285

Issue

Section

Theoretical and Experimental Cryobiology