Temperature Ranges of Phase Transformations in the Cryoprotective Media Components Determined by Thermoplastic Deformation Method

  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. L. Kirilyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
Keywords: thermoplastic deformation, fluidity limit, recrystallization, eutectic crystallization (melting), cryoprotectant

Abstract

Phase transformations in multicomponent cryoprotective media and their components were investigated during freeze-thawing using the method of thermoplastic deformation. The research objects included solution of cryoprotectant DMSO (10%) and the components of cryoprotective media being often utilized as a solvents for biologicals’ cryoprotective media (distilled water, physiological saline and the culture media: medium 199, Hanks’ solution, DMEM). The paper represents the temperature ranges of crystallization (melting) of eutectic concentration mixture for DMSO solution (from –87 to –67°C), eutectic crystallization (melting) of physiological solution and the culture media (from –37 to –21°C), recrystallization before melting of bulk ice (from –21 to –15°C) and following melting of bulk ice (from –15 to –4.5°C).

Published
2012-12-20
How to Cite
Gurina, T. M., & Kirilyuk, A. L. (2012). Temperature Ranges of Phase Transformations in the Cryoprotective Media Components Determined by Thermoplastic Deformation Method. Problems of Cryobiology and Cryomedicine, 22(4), 410-422. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/46
Section
Theoretical and Experimental Cryobiology