Post-Thaw Morphological Changes in Animal Erythrocytes

Authors

  • O. N. Denisova Kharkov State Zooveterinary Academy
  • L. G. Kuleshova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. G. Zemlyanskikh Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • L. A. Babijchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • G. F. Zhegunov Kharkov State Zooveterinary Academy

Keywords:

animal erythrocytes, cryoprotectant, stomatocytes, echinocytes

Abstract

Morphological changes in equine, bovine, and canine erythrocytes, when interacting with 20% dimethylsulfoxide (DMSO) and 30% polyethylene oxide with molecular mass of 1500 (PEO-1500) solutions, as well as after cell cryopreservation at the presence of the mentioned cryoprotectants have been studied in this research using light microscopy. Frozen-thawed erythrocytes of all animal species were established to be transformed into echinocytes under DMSO penetrative cryoprotectant effect and into stomatocytes under a non-penetrative one. The state of animal frozen-thawed erythrocytes when transferring them into physiological conditions was analysed.

Published

2007-06-15

How to Cite

Denisova, O. N., Kuleshova, L. G., Zemlyanskikh, N. G., Babijchuk, L. A., & Zhegunov, G. F. (2007). Post-Thaw Morphological Changes in Animal Erythrocytes. Problems of Cryobiology and Cryomedicine, 17(2), 147-155. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/514

Issue

Section

Theoretical and Experimental Cryobiology