Study of Morphofunctional Integrity of Platelets at Cryopreservation Stages

Authors

  • A. M. Kompaniets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. V. Knysh Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. A. Bogdanchikova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. E. Ovsyannikov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • M. L. Pogrebnyak Institute of Scintillation Materials of State Science and Technology Concern ‘Institute for Single Crystals’ of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

platelets, cryopreservation, cryoprotectants

Abstract

There were studied the effects of dimethyl sulfoxide (DMSO), dimethyl acetamide (DMAC), 1,2-propane diol (1,2-PD), their combination and two freezing programs on morphofunctional properties of human donor blood platelets at cryopreservation stages. The highest indices of platelet integrity were shown to be obtained at their cryopreservation with DMSO and when combining 1,2-PD with DMAC in 0.7 M final concentration during freezing according to the program with overcooling elimination. The results obtained testify to the necessity in elaborating the polycomponent cryoprotective media for platelet cryopreservation and perspectiveness in investigating special freezing programs with a controlled rate of passing through temperature interval of crystallisation.

Published

2006-06-15

How to Cite

Kompaniets, A. M., Knysh, O. V., Gurina, T. M., Bogdanchikova, O. A., Ovsyannikov, S. E., & Pogrebnyak, M. L. (2006). Study of Morphofunctional Integrity of Platelets at Cryopreservation Stages. Problems of Cryobiology and Cryomedicine, 16(2), 182–191. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/599

Issue

Section

Cryopreservation of Biological Resources