Study of Apoptotic Processes in Cells of Monocyte-Phagocyte System During Development of Adjuvant Arthritis Prior to and After Administration of Fetal Liver Cells

Authors

  • Anatoliy N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Ekaterina E. Yampolskaya Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Maksim V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Nikolay A. Bondarovich Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo24.03.249

Keywords:

apoptosis, monocyte-phagocyte system, adjuvant arthritis, fetal liver cells, cryopreservation

Abstract

Rheumatoid arthritis (RA) is one of the most widespread chronic inflammatory diseases of the joints with multifactor etiology. A crucial point in RA is the disorder of proliferation and apoptosis in immunocompetent cells as well as in the cells of monocyte-phagocyte system (MPS). Using the model of adjuvant arthritis (AA) in animals we comparatively assessed the development of apoptotic processes in MPS cells before and after administration of native and cryopreserved fetal liver cells (cFLCs). By means of Western blot analysis we showed the peculiarities of CD-95 cascade proteins expression in MPS cells during development of AA. After administration of cFLCs we found more pronounced proapoptotic effect, which degree depended on the period of their
administration.


Probl Cryobiol Cryomed 2014; 24(3):249-261.

Author Biographies

Anatoliy N. Goltsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Ekaterina E. Yampolskaya, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Maksim V. Ostankov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

Nikolay A. Bondarovich, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Cryopathophysiology and Immunology

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Published

2014-09-15

How to Cite

Goltsev, A. N., Yampolskaya, E. E., Ostankov, M. V., & Bondarovich, N. A. (2014). Study of Apoptotic Processes in Cells of Monocyte-Phagocyte System During Development of Adjuvant Arthritis Prior to and After Administration of Fetal Liver Cells. Problems of Cryobiology and Cryomedicine, 24(3), 249–261. https://doi.org/10.15407/cryo24.03.249

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Section

Cryomedicine, Clinical and Experimental Transplantology