Cryopreserved Multipotent Mesenchymal Stromal Cells Restore Fertility in Animals with Chronic Inflammation of Ovaries
DOI:
https://doi.org/10.15407/cryo26.01.084Keywords:
fertility, chronic inflammation, ovaries, cryopreservation, bone marrow, multipotent mesenchymal stromal cellsAbstract
The paper describes the influence of multipotent cryopreserved mesenchymal stromal bone marrow cells under conditions of intravenous administration alone and in combination with ‘Thymalinum’ (immune regulatory peptide complex from cattle thymus) on the fertility of mice with chronic inflammation of ovaries. The animals injected with a physiological saline were assumed as the control. To the day 21 following intravenous injection of cryopreserved multipotent mesenchymal stromal cells alone and in combination with ‘Thymalinum’ to the mice with chronic inflammation of ovaries an increase in a relative number of fertilized ova during superovulation (38 and 33%, respectively) was found, as well as in a pregnancy rate in natural estrous cycle (20 and 23% respectively) and the average litter number (98 and 83%, respectively) compared to the control animals. ‘Thymalinum’ application per se did not affect significantly the studied indices. The difference in body weight between the mice of experimental and control groups was observed only at early terms of observation, and to the day 15 it disappeared.
Probl Cryobiol Cryomed 2016; 26(1):84-92.
References
Abd-Allah S.H., Shalaby S.M., Pasha H.F. et al. Mechanistic action of mesenchymal stem cell injection in the treatment of chemically induced ovarian failure in rabbits. Cytotherapy 2013; 15: 64–75. CrossRef PubMed
Bailey M.M., Boohaker J.G., Sawyer R.D. et al. Exposure of pregnant mice to chromium picolinate results in skeletal defects in their offspring. Birth Defects Research. Part B, Developmental and Reproductive Toxicology 2006; 77(3): 244–249. CrossRef PubMed
Drannik G.N. Clinical Immunology and allerhology. Moscow: Med. Inform. Agentstvo; 2003.
Dubossarskaya Z.M., Mylyanovskyy A.I., Kolyadenko V.G. Chronic inflammatory processes in women sexual organs. Kiev: Zdorovye; 2003.
Egorov N.S. Practical microbiology. Moscow: Moscow State University; 1976.
Grischenko N.G., Grischenko V.I., Smoljaninova E.I. et al. Effect of placental cryoextract on superovulation induction in mice with chronic ovary inflammation. Problems of Cryobiology and Cryomedicine 2010; 20 (3): 327–337.
Frenette P.S., Pinho S., Lucas D., Scheiermann C. Mesenchymal stem cell: keystone of the hematopoietic stem cell niche and a stepping-stone for regenerative medicine. Ann Rev Immunol 2013; 31: 285–316. CrossRef PubMed
Fu X., He Y., Xie C. et al. Bone marrow mesenchymal stem cell transplantation improves ovarian function and structure in rats with chemotherapy-induced ovarian damage. Cytotherapy 2008; 10: 353–363. CrossRef PubMed
Hruleva N.V., Csherbatjuk T.G., Potekhina Ju.P. Peculiarities of a female reproductive system response to the superovulation stimulation in the IVF cycle. Sovremennye Tehnologii v Meditsine 2011; 1: 20–23.
Huraseva A.B. The role of hereditary factors in the formation of fetal weight. System Analisis and Management in Biomedical Systems 2008; 7(2): 375–378.
Komarova S., Roth J., Alvarez R. et al. Targeting of mesen-chymal stem cells to ovarian tumors via an artificial receptor. Journal of Ovarian Research 2010; 3(12): 12–18. CrossRef PubMed
Liu J., Zhang H., Zhang Y. et al. Homing and restorative effects of bone marrow-derived mesenchymal stem cells on cisplatin injured ovaries in rats. Mol Cells 2014; 37(12): 865–872. CrossRef PubMed
Mank M., editor. Developmental biology of mammals. Methods. Moscow; 1990.
Medical and demographic situation and organization of medical care in 2010: results of the health system and implementation of the Program of Economic Reforms in 2010–2014 "Prosperous Society, Competitive Economy, Effective State". Kiev: Ministry of Health of Ukraine; 2011.
Nazarova G.G., Evsikov V.I. Evolutionary ecology of animal fertility: the fitness of the progeny is determined by their prenatal development (by the example of the european water vole, Arvicola Terrestris L.). Vavilov Journal of Genetics and Breeding 2011; 15 (3): 485–492.
Scherbina N.A., Gradil O.G. Assessment of age changes of ovarian reserve in infertile women. Tavricheskiy Mediko-Biologicheskiy Vestnik 2013; 16 (2): 140–144.
Sohni A., Verfaillie C.M. Mesenchymal stem cells migration, homing and tracking. Stem Cells Int 2013; 2013: 130763.
Takehara Y., Yabuuchi A., Ezoe K. et al. The restorative effects of adipose-derived mesenchymal stem cells on damaged ovarian function. Laboratory Investigation 2013; 93: 181–193. CrossRef PubMed
Vijayalakshmi V., Soundarya L. Promise(s) of using mesenchymal stem cells in reproductive disorders. Indian J Med Res 2014; 140 (1): 98–105.
Volkova N.O. Study of morphological characteristics of cryopreserved cell cultures of stromal origin. Problems of Cryobiology and Cryomedicine 2012; 22 (2): 118–125.
Volkova N.Ð., Yukhta M.S., Yurchuk Т.Ð. et al. Multipotent mesenchymal stromal cells of bone marrow in therapy of chronic inflammation of the murine ovaries. Biotechnologia Acta 2014; 7(5): 35–42. CrossRef
Weina L., Qixuan X., Junwen Q. et al. Effect of mesenchymal stem cell transplantation on immunological injury of the ovary in mice. J South Med Univ 2011: 31(5): 825–829.
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Copyright (c) 2020 Nataliya O. Volkova, Mariya S. Yukhta, Larisa V. Stepanyuk, Lyudmila G. Chernyshenko
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