Effect of Cryoprotective Solutions and Their Components on Intensity of Lipid Peroxidation and Antioxidant Activity in Leukocytes at Cryopreservation
Keywords:leukocytes, cryoprotectants, cryopreservatives, lipid peroxidation, antioxidant activity
Using chemiluminescence method it was shown that after addition of several cryoprotectants 1,2-propane diol (21% initial concentration hereinafter and added 1:1) and dimethyl sulfoxide (8%) to leukocyte suspension the level of LPO and antioxidant activity decreased. Presence of glycerol (7%) and hexa-methylene bis-tetra hydroxyethyl urea (30%) resulted in simultaneous stimulation of LPO and antioxidant systems. Hydroxyethylated starch (5.7%) and gelatine (molecular weight of 16,000; 7.5%) increase LPO. After storage in combined cryoprotective media at –10, –20 and –40°C the leukocytes revealed the activation of antioxidant systems on the background of stable LPO indices, that, probably, contributed to morphofunctional preservation of 75% of cells. Probl Cryobiol Cryomed 2013; 23(1):49–57.
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