Effect of Cryoprotective Solutions and Their Components on Intensity of Lipid Peroxidation and Antioxidant Activity in Leukocytes at Cryopreservation

Andrey N. Khudyakov, Tatyana V. Polezhaeva, Oksana O. Zaitseva, Denis S. Laptev, Olga N. Solomina, Andrey A. Kostyaev

Abstract


Using chemiluminescence method it was shown that after addition of several cryoprotectants 1,2-propane diol (21% initial concentration hereinafter and added 1:1) and dimethyl sulfoxide (8%) to leukocyte suspension the level of LPO and antioxidant activity decreased. Presence of glycerol (7%) and hexa-methylene bis-tetra hydroxyethyl urea (30%) resulted in simultaneous stimulation of LPO and antioxidant systems. Hydroxyethylated starch (5.7%) and gelatine (molecular weight of 16,000; 7.5%) increase LPO. After storage in combined cryoprotective media at –10, –20 and –40°C the leukocytes revealed the activation of antioxidant systems on the background of stable LPO indices, that, probably, contributed to morphofunctional preservation of 75% of cells.


Probl Cryobiol Cryomed 2013; 23(1):49–57.


Keywords


leukocytes; cryoprotectants; cryopreservatives; lipid peroxidation; antioxidant activity

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