Effect of Cryoprotective Solutions and Their Components on Intensity of Lipid Peroxidation and Antioxidant Activity in Leukocytes at Cryopreservation

Authors

  • Andrey N. Khudyakov Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Tatyana V. Polezhaeva Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Oksana O. Zaitseva Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Denis S. Laptev Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Olga N. Solomina Physiology Institute at the Scientific Center affiliated to the Ural branch of the Russian Academy of Sciences, Kirov
  • Andrey A. Kostyaev Kirov Research Institute of Hematology and Blood Transfusion, Kirov

Keywords:

leukocytes, cryoprotectants, cryopreservatives, lipid peroxidation, antioxidant activity

Abstract

Using chemiluminescence method it was shown that after addition of several cryoprotectants 1,2-propane diol (21% initial concentration hereinafter and added 1:1) and dimethyl sulfoxide (8%) to leukocyte suspension the level of LPO and antioxidant activity decreased. Presence of glycerol (7%) and hexa-methylene bis-tetra hydroxyethyl urea (30%) resulted in simultaneous stimulation of LPO and antioxidant systems. Hydroxyethylated starch (5.7%) and gelatine (molecular weight of 16,000; 7.5%) increase LPO. After storage in combined cryoprotective media at –10, –20 and –40°C the leukocytes revealed the activation of antioxidant systems on the background of stable LPO indices, that, probably, contributed to morphofunctional preservation of 75% of cells. Probl Cryobiol Cryomed 2013; 23(1):49–57.

Author Biography

Andrey A. Kostyaev, Kirov Research Institute of Hematology and Blood Transfusion, Kirov

Кировский НИИ гематологии и переливания крови ФМБА России

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Published

2013-03-15

How to Cite

Khudyakov, A. N., Polezhaeva, T. V., Zaitseva, O. O., Laptev, D. S., Solomina, O. N., & Kostyaev, A. A. (2013). Effect of Cryoprotective Solutions and Their Components on Intensity of Lipid Peroxidation and Antioxidant Activity in Leukocytes at Cryopreservation. Problems of Cryobiology and Cryomedicine, 22(1), 49-57. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/9

Issue

Section

Theoretical and Experimental Cryobiology