Posthypertonic Lysis of Red Blood Cells When Changing pH and Composition of Rehydrating Medium

Authors

  • O. A. Olejnic
  • V. V. Ramazanov
  • V. A. Bondarenko

Abstract

We have investigated the effect of rehydration medium composition on cells’ sensitivity to posthypertonic lysis. It has been shown that hypertonic rehydration media prevent red cell damages. Dilution of these media results in an increase of the hemolysis level. Application of PEG-1500 cryoprotectant allows to increase the cell integrity.

Author Biographies

O. A. Olejnic

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

V. V. Ramazanov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

V. A. Bondarenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Downloads

Published

2002-09-23

How to Cite

Olejnic, O. A., Ramazanov, V. V., & Bondarenko, V. A. (2002). Posthypertonic Lysis of Red Blood Cells When Changing pH and Composition of Rehydrating Medium. Problems of Cryobiology and Cryomedicine, (3), 30–35. Retrieved from http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/955

Issue

Section

Theoretical and Experimental Cryobiology