TY - JOUR AU - Bohuslavskyi, Konstantin I. AU - Alabedalkarim, Natalia M. AU - Pakhomov, Alexsandr V. AU - Bozhok, Galyna A. PY - 2016/12/23 Y2 - 2024/03/28 TI - Effect of Incubation of PK-15 Line Cells with Cryoprotectants on Amount of Gal-alpha-1,3-Gal Epitopes JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 26 IS - 4 SE - Theoretical and Experimental Cryobiology DO - 10.15407/cryo26.04.331 UR - http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1242 SP - 331-339 AB - <p>Transplantation of bioprostheses derived from bovine and porcine tissues is an advanced method of correcting defects in blood vessels and valves, however when using it a hyperacute immune response associated with the presence of Gal-alpha- 1,3-Gal (α-Gal epitope) on the surface of cell membranes can develop. We have studied the effect of penetrating and nonpenetrating cryoprotectants on the amount of α-Gal epitopes on membranes of PK-15 cell line of porcine origin. Cell labeling with BSIB4 lectin conjugated with FITC and flow cytometry revealed a significant increase in the number of fluorescent cells after incubation with various concentrations of dimethyl sulfoxide and polyethylene glycol with MW of 1500 (PEG-1500). The revealed effect may be associated with an additional exposure of α-Gal epitopes on cell membrane, locally deformed due to the action of cryoprotectants.</p><p><em>Probl Cryobiol Cryomed 2016; 26(4): 331–339</em><em></em></p> ER -