TY - JOUR AU - Isayenko, Olena AU - Knysh, Oksana AU - Popov, Mykola AU - Minukhin, Valeriy AU - Babych, Eugeny AU - Peretyatko, Olena PY - 2021/07/15 Y2 - 2024/03/29 TI - Impact of Biologically Active Complexes of L. rhamnosus GG and S. boulardii After Storage Under Low Temperatures on Biofilm Forming Ability in Corynebacteria JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 31 IS - 2 SE - Theoretical and Experimental Cryobiology DO - 10.15407/cryo31.02.127 UR - http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1727 SP - 127-138 AB - <p>This paper describes the temperature regimens and shelf life of biologically active complexes (structural components and metabolites) of <em>Lactobacillus rhamnosus</em> GG and <em>Saccharomyces boulardii</em>, which have antimicrobial activity and reduce biofi lm formation in pathogenic agents of <em>Corynebacterium spp</em>. Preservation of biological activity of complexes after 6-month storage (observation period) at (−23 ± 1)°C and for 60 days (observation period) under hypothermia at (4 ± 1)°C has been demonstrated. The degree of inhibition of biofi lm formation in pathogenic corynebacteria depended on sensitivity of the <em>Corynebacterium spp</em>. test-culture strain to products of microbial origin. A mixture of lactobacillus and saccharomycete metabolites displayed weak biofi lm formation by toxicogenic strains of corynebacteria (p &lt; 0.05). Our fi ndings testifi ed to a possible use of biologically active substances, stored at 4 and −23°C in designing the <em>L. rhamnosus</em> GG and <em>S. boulardii</em> complexes and in technological processes of their production to prevent the persistence of the diphtheria pathogens.</p><p><em>Probl Cryobiol Cryomed 202</em><em>1</em><em>; 3</em><em>1</em><em>(2): 127–138</em></p> ER -