TY - JOUR AU - Gurina, T. M. AU - Kirilyuk, A. L. PY - 2012/12/20 Y2 - 2024/03/29 TI - Temperature Ranges of Phase Transformations in the Cryoprotective Media Components Determined by Thermoplastic Deformation Method JF - Problems of Cryobiology and Cryomedicine JA - Probl Cryobiol Cryomed VL - 22 IS - 4 SE - Theoretical and Experimental Cryobiology DO - UR - http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/46 SP - 410-422 AB - <p>Phase transformations in multicomponent cryoprotective media and their components were investigated during freeze-thawing using the method of thermoplastic deformation. The research objects included solution of cryoprotectant DMSO (10%) and the components of cryoprotective media being often utilized as a solvents for biologicals’ cryoprotective media (distilled water, physiological saline and the culture media: medium 199, Hanks’ solution, DMEM). The paper represents the temperature ranges of crystallization (melting) of eutectic concentration mixture for DMSO solution (from –87 to –67°C), eutectic crystallization (melting) of physiological solution and the culture media (from –37 to –21°C), recrystallization before melting of bulk ice (from –21 to –15°C) and following melting of bulk ice (from –15 to –4.5°C).</p> ER -