http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/issue/feedProblems of Cryobiology and Cryomedicine2025-04-07T09:14:03+02:00Anatoliy M. Goltsevjournal@cryo.org.uaOpen Journal Systems<p>The Journal was established in 1985. Till 1991 it was published under title Kriobiologia, and Problemy kriobiologii/Problems of Cryobiology from 1991 till 2012. (<a href="/journal/index.php/probl-cryobiol-cryomed/at_a_glance">Journal at a Glance</a>)</p> <p>The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine.</p> <p>The Editorial Board takes the responsibility to provide a comprehensive peer-review of all submitted manuscripts. The Journal team strictly follows publishing ethics and standards to ensure high quality of scholarly publications. The Journal endorses the International Committee of Medical Journal Editors (ICMJE) <a href="http://www.icmje.org/recommendations/" target="_self">Recommendations for the Conduct, Reporting, Editing and Publication of Scholarly Work in Medical Journals</a>. (<a href="/journal/index.php/probl-cryobiol-cryomed/pubethics">Read more about Publishing Policy and Ethics</a>).</p> <p>Members of our Editorial Board are the specialists in various fields of low temperature biology and medicine from Ukraine and all over the world. (<a href="https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/about/editorialTeam">Editorial Board</a>).</p> <p>We do not charge the authors for article submission and/or processing and do not provide off-prints. The operation of the Journal is supported by National Academy of Sciences of Ukraine.</p> <p>Articles published in Problems of Cryobiology and Cryomedicine are Open-Access articles distributed under the terms and conditions of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/4.0/">CC BY</a>). We provide immediate open access to its content adhering to the principle that making research freely available to the public supports a greater global exchange of knowledge, increase visibility and readership. Use and distribution of the publications are permitted, retaining the credits to the authors and the Journal. The Journal holds the exclusive license to be the first publisher of the articles in print form and on-going non-exclusive license to disseminate the published content in various ways. The published version of the article could be deposited by authors to institutional or public repositories immediately on publication, stating a link to the URL of the published article on the journal's web-site.</p> <p>Our Journal is indexed/abstracted in <a href="http://www.scopus.com">Scopus</a>, <a href="http://thomsonreuters.com/en/products-services/scholarly-scientific-research/scholarly-search-and-discovery/zoological-record.html">Zoological Records</a>, <a href="http://www.cas.org/">Chemical Abstracts</a>, <a href="https://www.ebscohost.com/discovery?_ga=2.208532498.1058102411.1521647075-1463563202.1521647075">EBSCOhost</a>, <a href="http://bd.viniti.ru/">VINITI</a>, <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis64r_81/cgiirbis_64.exe?C21COM=F&I21DBN=REF_EX&P21DBN=REF&S21CNR=20&Z21ID=">Dzherelo </a>(Ukrainian Scientific Abstracts) and deposited in <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?Z21ID=&I21DBN=UJRN&P21DBN=UJRN&S21STN=1&S21REF=10&S21FMT=juu_all&C21COM=S&S21CNR=20&S21P01=0&S21P02=0&S21P03=PREF=&S21COLORTERMS=0&S21STR=KrioBiol">Vernadsky National Library of Ukraine Repository</a> (2002-now) and Google Books (<a href="https://books.google.co.jp/books?id=CSfuSZsuprkC&dq=kriobiologiya&hl=uk&source=gbs_all_issues_r&cad=1&atm_aiy=1970#all_issues_anchor">1975-1990</a>; <a href="https://books.google.com.ua/books?id=B326AAAAIAAJ&dq=problems+of+cryobiology&hl=uk&source=gbs_navlinks_s">1990-2002</a>).</p> <div id="gtx-anchor" style="position: absolute; visibility: hidden; left: 1473.54px; top: 296.181px; width: 9.88892px; height: 18.8889px;"> </div> <div class="jfk-bubble gtx-bubble" style="visibility: visible; left: 575px; top: 325px; opacity: 1;" role="alertdialog" aria-describedby="bubble-2"> <div id="bubble-2" class="jfk-bubble-content-id"> <div id="gtx-host" style="min-width: 200px; max-width: 400px;"> </div> </div> <div class="jfk-bubble-closebtn-id jfk-bubble-closebtn" tabindex="0" role="button" aria-label="Close"> </div> <div class="jfk-bubble-arrow-id jfk-bubble-arrow jfk-bubble-arrowup" style="left: 1470.56px;"> <div class="jfk-bubble-arrowimplbefore"> </div> <div class="jfk-bubble-arrowimplafter"> </div> </div> </div>http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2001Pilot Study of Hibernation Patterns in Noctule Bats (Nyctalus noctula): Influence of Group Size2025-04-04T18:30:29+02:00Anton Vlaschenkovlaschenko@gmail.comMarharyta Moiseienkoprobl.cryobiol.cryomed@gmail.comValeria Bohodistprobl.cryobiol.cryomed@gmail.comAnastasia Domanskaprobl.cryobiol.cryomed@gmail.comAnatolii Goltvyanskiyprobl.cryobiol.cryomed@gmail.comOleksandr Nahlovprobl.cryobiol.cryomed@gmail.com<p>This study investigated the influence of group size on the hibernation patterns of adult males of Nyctalus noctula during hibernation. Bats were divided into three categories (solitary, groups of six, and groups of sixteen) and nine experimental groups comprising one individual with temperature logger per group (iButton). Skin temperature (Ts) was monitored every five minutes. The results showed that bats in larger groups (the group of sixteen) entered longer and more stable torpor, with no instances of full arousal (Ts >20°C). In contrast, solitary bats exhibited more frequent arousals. All bats entered deep torpor after the first nine days. No significant differences in body mass loss were found between the groups. The findings highlight the importance of group size in reducing arousals during hibernation, and the role of social thermoregulation in conserving energy.</p> <p> </p> <p><em>Probl Cryobiol Cryomed 2024; 34(4):251–260</em></p>2025-04-04T00:00:00+02:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2002Optimization of Lyophilization Modes for Culture Medium Used in Mycobacterial Cultivation2025-04-04T19:02:42+02:00Andriy Zavhorodniybw.pochta@gmail.comViktor Bilushkobw.pochta@gmail.comSvitlana Pozmohovaprobl.cryobiol.cryomed@gmail.comAnatoliy Paliypaliy.dok@gmail.comMykola Kalashnykprobl.cryobiol.cryomed@gmail.comOlena Pavlichenkoprobl.cryobiol.cryomed@gmail.comKarina Sviridovaprobl.cryobiol.cryomed@gmail.comOleksandra Tischenkoprobl.cryobiol.cryomed@gmail.com<p>The paper presents the results of improving the lyophilization mode of egg-based culture medium for mycobacterial cultivation. The freezing of culture medium of 200 cm<sup>3</sup> volume at -30°C was established to provide more optimal conditions for lyophilization than other options we suggested here (200 cm<sup>3</sup> at –20°C and 300 cm<sup>3</sup> at –20 and –30°C). This enables to reduce the number of rejected vials and preserve the elective properties of the medium. Other freezing modes occurred to be less efficient. Our findings also showed the use of recommended modes for lyophilization as capable to accelerate the appearance of first signs of mycobacterial growth by 1–2 days.</p> <p><em>Probl Cryobiol Cryomed 2024; 34(4):261–267</em></p> <p> </p>2025-04-04T00:00:00+02:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2003Hsp70 as a Potential Biomarker of Tolerization of Cryopreserved Mononuclear Cells-Derived Dendritic Cells2025-04-04T19:33:50+02:00Anatoliy Goltsevcryopato@gmail.comTetyana Dubravacryopato@gmail.comOlena Lutsenkocryopato@gmail.comYulia Gaevskacryopato@gmail.comMykola Bondarovychcryopato@gmail.comMaksym Ostankovcryopato@gmail.com<p>The work investigated the effect of three cryopreservation regimens of bone marrow mononuclear cells (MNCs) of CBA/H mice under the protection of a 10% dimethyl sulfoxide solution with a slow cooling rate of 1 deg / min: regimen 1 – to –80ºС; regimen 2 – to –40ºС; regimen 3 – to –25ºС with subsequent immersion of samples into liquid nitrogen (–196ºС) on the expression rate of heat shock protein with a molecular weight of 70 kDa (Hsp70) in MNCs and dendritic cells (DCs) obtained from them in vitro. The DCs tolerogenic potential was assessed after their use in adaptive therapy in mice with adjuvant arthritis by the ability to induce regulatory T cells (Treg) in spleen and minimize the arthritis index, as a clinical index of the pathology development. The dependence of expression rate of the Hsp70 protein in MNCs on the freezing mode was proven, as well as the signifi cance of this protein content in the manifestation of tolerogenic potential of DCs formed from them in vitro. The ability of cryopreservation according to regimen 2 to increase the Hsp70 content in MNCs was shown. The functional potential of DCs grown from cryopreserved MNCs with regimen 2 demonstrated their higher tolerogenic activity compared to DCs derived from native MNCs and cryopreserved with the regimens 1 and 3 MNCs. The maximum content of Hsp70 in DCs when using regimen 2 was consistent with that of Treg formed in animals with adjuvant arthritis after the administration of these cells, as well as it was accompanied by the most pronounced normalization of clinical signs of pathology. The findings demonstrate the ability of cryopreservation to regulate the Hsp70 expression rate as well as tolerogenic potential of DCs formed from cryopreserved precursors, increasing their use effectiveness in adaptive therapy of autoimmune diseases.</p> <p><em>Probl Cryobiol Cryomed 2024; 34(4):268–281</em></p>2025-04-04T00:00:00+02:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2004Therapy With Cryopreserved and Lyophilized Cord Blood Leukoconcentrates in Experimental Atopic Dermatitis2025-04-04T19:56:25+02:00Hanna Kovalcryopato@gmail.comOlena Lutsenkocryopato@gmail.comMykola Bondarovychcryopato@gmail.comIgor Grishacryopato@gmail.comLarysa Sokilcryopato@gmail.comMaksym Ostankovcryopato@gmail.comAnatoliy Goltsevcryopato@gmail.com<p><strong><em>Probl Cryobiol Cryomed 2024; 34(4):282–286</em></strong></p>2025-04-04T00:00:00+02:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/200548th Annual International Conference of Young Scientists "Cold in biology and medicine: current problems in cryobiology, transplantology, and biotechnology", Kharkiv, May, 15–16 2024 2025-04-05T12:03:13+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl Cryobiol Cryomed 2024; 34(4):287–289</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2006Reduction of the Toxicity of CPAs is Without Compromising the Cryoprotection in Sea Urchin Eggs (Part 1)2025-04-05T12:09:03+02:00Uxía Rodríguez-Gavieirorodriguezgavieirouxia@gmail.comSara Camposprobl.cryobiol.cryomed@gmail.comEstefanía Pereira-Pintoprobl.cryobiol.cryomed@gmail.comEstefanía Paredesprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):290</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2007Reduction of the Toxicity of CPAs is Without Compromising the Cryoprotection in Sea Urchin Eggs (Part 2)2025-04-05T12:28:16+02:00Uxía Rodríguez-Gavieirorodriguezgavieirouxia@gmail.comSara Camposprobl.cryobiol.cryomed@gmail.comEstefanía Pereira-Pintoprobl.cryobiol.cryomed@gmail.comEstefanía Paredesprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):290</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2008Stability of Mammalian Erythrocytes Under Varying Osmotic Conditions of the Medium2025-04-05T13:23:54+02:00Olena Chabanenkoprobl.cryobiol.cryomed@gmail.comNatalia Yershovaprobl.cryobiol.cryomed@gmail.comSerhii Yershovprobl.cryobiol.cryomed@gmail.comNataliia Shpakovaprobl.cryobiol.cryomed@gmail.com<p>Probl Cryobiol Cryomed 2024; 34(4):291</p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2009Low Temperatures Influence the Preservation of Regenerative Properties of Hyaluronic Acid with Different Molecular Weight2025-04-05T13:29:34+02:00Anatolii Seliutaprobl.cryobiol.cryomed@gmail.comEduard Nardidprobl.cryobiol.cryomed@gmail.comAnna Polyakovaprobl.cryobiol.cryomed@gmail.comTetiana Gurinaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):291</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2010Cryopreservation of Shoot Tips of Valeriana jatamansi, a High Value Threatened Medicinal Plant of India2025-04-05T13:35:21+02:00Ravi Gowthamigowthamir111@gmail.comSubhash Chanderprobl.cryobiol.cryomed@gmail.comAnuradha Agrawalprobl.cryobiol.cryomed@gmail.comNeelam Sharmaprobl.cryobiol.cryomed@gmail.comKailash Chandra Bhatprobl.cryobiol.cryomed@gmail.comAnju Mahendru Singhprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):292</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2011A Wide Spectrum Droplet Vitrification Cryopreservation Protocol for Conservation of Hops (Humulus lupulus)2025-04-05T13:47:07+02:00Era Malhotravaidya.era@gmail.comSuresh Maliprobl.cryobiol.cryomed@gmail.comShreya Sharmaprobl.cryobiol.cryomed@gmail.comSangita Bansalprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4): 292</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2012Effect of Co-Culturing Neural Cells with MSCs on Spheroid Formation and Their Recovery After Cryopreservation2025-04-06T13:26:56+02:00Svitlana Vsevolodskaprobl.cryobiol.cryomed@gmail.comOlha Ochenashkoprobl.cryobiol.cryomed@gmail.comOlha Maiorovaprobl.cryobiol.cryomed@gmail.comOleksandr Sukachprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):293</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2013Short-Term Storage of Core-Shell Encapsulated MSCs at Ambient Temperature2025-04-06T14:03:01+02:00Oleksandra Hubeniaprobl.cryobiol.cryomed@gmail.comSara Leal-Marinprobl.cryobiol.cryomed@gmail.comNatalia Trufanovaprobl.cryobiol.cryomed@gmail.comBirgit Glasmacherprobl.cryobiol.cryomed@gmail.comOleksandr Petrenkoprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):293</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2014The Experience of Using Cryotechnology in Animal Breeding to Ensure Food Security in Ukraine2025-04-06T14:30:42+02:00Anastasiia Bogdaniukprobl.cryobiol.cryomed@gmail.comMaryna Petrushkoprobl.cryobiol.cryomed@gmail.comVolodymyr Garkaviiprobl.cryobiol.cryomed@gmail.com<p>Probl Cryobiol Cryomed 2024; 34(4):294</p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2015Different Approaches for Mesenchymal Stromal Cells Isolation from Wharton’s Jelly Tissue2025-04-06T14:51:19+02:00Ruslana Oberemokprobl.cryobiol.cryomed@gmail.comNatalia Trufanovaprobl.cryobiol.cryomed@gmail.comOleh Trufanovprobl.cryobiol.cryomed@gmail.comOlena Revenkoprobl.cryobiol.cryomed@gmail.comOleksandr Petrenkoprobl.cryobiol.cryomed@gmail.com<p>Probl Cryobiol Cryomed 2024; 34(4): 294</p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2016The Use of Cryopreserved Medications from the Feto-Placental Complex as a Method for Treating Infertility Caused by Chronic Endometritis: a Case Report2025-04-06T15:02:37+02:00Oleksandr Feskovprobl.cryobiol.cryomed@gmail.comLina Konopliaprobl.cryobiol.cryomed@gmail.comAnna Feskovaprobl.cryobiol.cryomed@gmail.com<p>Probl Cryobiol Cryomed 2024; 34(4):295</p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2017Therapeutic Effect of Umbilical Cord Blood and Placenta in the Treatment of Chronic Endometritis in Patients with Repeated Implantation Failures2025-04-06T16:40:15+02:00Oleksandr Feskovprobl.cryobiol.cryomed@gmail.comYevhenia Zhylkovaprobl.cryobiol.cryomed@gmail.comLina Konopliaprobl.cryobiol.cryomed@gmail.comOlena Blazhkoprobl.cryobiol.cryomed@gmail.comIryna Bezpechnaprobl.cryobiol.cryomed@gmail.comAnna Feskovaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):295</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2018The First Experience of Reproductive Function Restoration in Patients with Asherman's Syndrome Using Autologous Mesenchymal Stem Cells2025-04-06T17:07:47+02:00Mykola Kozubkozub061979@gmail.comMaksym Kozubkozub061979@gmail.comKseniia Skybinaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):296</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2019Long-Term Storage of Seeds from Plant Gene Pool Samples in the National Depository2025-04-06T17:20:59+02:00Yuliia Chernobaijuliaonishchenko2112@gmail.comViktor Riabchunprobl.cryobiol.cryomed@gmail.comNataliia Kuzmyshynaprobl.cryobiol.cryomed@gmail.comTetyana Shyianovaprobl.cryobiol.cryomed@gmail.comRoman Bohuslavskyiprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):296</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2020Thermoplastic Analysis of State Diagrams of Cryoprotective Solutions2025-04-06T17:33:28+02:00Stanislav Sevastianovprobl.cryobiol.cryomed@gmail.comOleksandr Osetskyprobl.cryobiol.cryomed@gmail.comTetiana Gurinaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):297</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2021Optimization of Hypothermic Storage of Canine Erythrocytes: the Role of Acetylcysteine in Increasing Mechanical Stability2025-04-06T17:38:23+02:00Karyna Hrebeniukkarinavel451@gmail.comOlha Denysovaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):297</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2022Study of the Interaction of Antimicrobial Peptides, in Particular Gramicidin S With Cell Membranes2025-04-06T17:44:50+02:00Igor Perepelitsaigorperepelitsa06@gmail.comVolodymyr Berestprobl.cryobiol.cryomed@gmail.comGalyna Bozhokprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):298</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2023Seasonal Dynamics of Changes in the Freezing Point of Natural Milk as a Criterion for Determining the Possible Amount of Added Water2025-04-06T19:08:45+02:00Nataliia Ruskoprobl.cryobiol.cryomed@gmail.comValeriia Ponomarovadrfox420@gmail.comIgor Korkhprobl.cryobiol.cryomed@gmail.comAnatoliy Paliypaliy.dok@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):298</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2024Preparation of Ram Sperm Using Minimal Application Gradient for In Vitro Fertilization2025-04-06T19:16:42+02:00Oksana Lyzohuboksanalyzohub@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):299</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2025Selection of Sugars for Cryoprotective Media on MCF-7 Breast Cancer Cell Cultures 2025-04-06T19:20:24+02:00Bohdan Kopiakkopyakbohdan@gmail.comOlena Perepelitsinaprobl.cryobiol.cryomed@gmail.comMykhailo Sydorenkoprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):299</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2026Cultivation and Cryopservation of Protozoan Intestinal Parasites2025-04-06T19:28:50+02:00Igor Kyrychenkokiricheigor@gmail.comMykhailo Biriukovbirukovmaik@gmail.comSergiy Pokhilprobl.cryobiol.cryomed@gmail.comOlena Tymchenkoprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):300</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2027Experimental Justification of Effectiveness of Cord Blood Serum-Derived Biological Product for Correction of Autoimmune Thyroiditis2025-04-06T19:38:16+02:00Yuliia Kurylkoukurilko1@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):300</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2028Cryoresistance of Antarctic lichen Umbilicaria umbilicarioides Assessed by a Constant Rate Cooling Combined with Chlorophyll Fluorescence Data2025-04-06T19:46:26+02:00Jiří Sekerák Jr 507924@mail.muni.czJosef Hájekprobl.cryobiol.cryomed@gmail.comAnton Puhovkinprobl.cryobiol.cryomed@gmail.comMiloš Bartákprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):301</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2029Cryopreservation of Allium tuberosum Rottler ex Sprengel2025-04-06T19:57:12+02:00Subhash Chandersinghariya43gdrive1@gmail.comRavi Gowthamigowthamir111@gmail.comRuchira Pandeyprobl.cryobiol.cryomed@gmail.comAnuradha Agrawalprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):301</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2030Morphological Indices of Spleen Under Short-Term Hypothermic Exposure2025-04-06T20:09:09+02:00Ruslan Aminovaminovruslan947@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):302</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2031Impact of Cryosublimation on Cord Blood Serum Composition2025-04-06T20:12:44+02:00Anastasiia Skorykprobl.cryobiol.cryomed@gmail.comMariia Shevchenkoprobl.cryobiol.cryomed@gmail.comDmytro Salnykovprobl.cryobiol.cryomed@gmail.comOlga Prokopiukprobl.cryobiol.cryomed@gmail.comOlga Gorinaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):302</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2032Cord Blood Serum Composition After Low-Temperature Storage at –40°C2025-04-06T20:21:14+02:00Dmytro Salnykovprobl.cryobiol.cryomed@gmail.comOlga Gorinaprobl.cryobiol.cryomed@gmail.comOlga Prokopiukprobl.cryobiol.cryomed@gmail.comTetyana Mykhalchukprobl.cryobiol.cryomed@gmail.comMariia Shevchenkoprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):303</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2033Transcriptomic Analysis of Genes Expressed After Carotid Endarterectomy: Searching for New Pathways for Cerebral Ischemic Tolerance2025-04-06T20:51:47+02:00Marek Furmanmarek.furman12@gmail.comVladimir Sihotskyprobl.cryobiol.cryomed@gmail.comMichal Viragprobl.cryobiol.cryomed@gmail.comIvan Kopolovetsprobl.cryobiol.cryomed@gmail.comMiroslava Nemethovaprobl.cryobiol.cryomed@gmail.comRastislav Muchaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):303</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2034Cryopreservation Challenges and Cryoprotectant Efficacy in Cyprinodon variegatus Embryos2025-04-06T21:04:24+02:00Guillermo Manuel Santosguille.snt@gmail.comFrancisco Rochaprobl.cryobiol.cryomed@gmail.comEstefanía Paredesprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):304</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2035Migration Response of Astrocytes to the Effect of Conditioned Medium From Rat Bone Marrow Mesenchymal Stem Cells2025-04-06T21:14:28+02:00Eva Székiováeszekiova@gmail.comZuzana Michalováprobl.cryobiol.cryomed@gmail.comJuraj Blaškoprobl.cryobiol.cryomed@gmail.comIvo Vanickýprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):304</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2036Molecular Dynamics Study of Ternary Water-NaCl-DMSO Mixtures During Freezing and Thawing2025-04-06T21:31:58+02:00Ivan Klbikivkoklbik@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):305</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2037Tomato Seed Yield Depending on Pre-Sowing Treatment2025-04-06T21:36:12+02:00Maryna Shapkoprobl.cryobiol.cryomed@gmail.comNadiia Shevchenkoprobl.cryobiol.cryomed@gmail.comGalyna Kovalenkoprobl.cryobiol.cryomed@gmail.comOleksandr Kutsprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):305</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2038Effect of Cold on Blood Circulation in People With Different Somatotypes and Blood Pressure Levels2025-04-06T21:45:26+02:00Liudmyla Horbanhorban@tdmu.edu.ua<p><em>Probl Cryobiol Cryomed 2024; 34(4):306</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2039Effects of AIMР1/p43 and EMAP II Proteins on Malignant Tumor Cells2025-04-06T21:50:10+02:00Mariia Kolomiietskolomietsmaria8@gmail.comVolodymyr Zayetsprobl.cryobiol.cryomed@gmail.comLeonid Zaikaprobl.cryobiol.cryomed@gmail.comOlga Bolsunovaprobl.cryobiol.cryomed@gmail.comOleksandr Kornelyukprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):306</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2040Hormonal Status of Aged Rats with Diet-Induced Obesity After Umbilical Cord Blood Administration2025-04-06T22:01:15+02:00Olena Bondarenkoprobl.cryobiol.cryomed@gmail.comVladyslav Babiychukprobl.cryobiol.cryomed@gmail.comLyudmyla Babiychukprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):307</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2041Optimization of Human Umbilical Cord Mesenchymal Stem Cell Cryopreservation Protocol2025-04-06T22:06:27+02:00Ilona Paliiprobl.cryobiol.cryomed@gmail.comOlesia Redkoredko_os@tdmu.edu.uaSofiia Lechachenkoprobl.cryobiol.cryomed@gmail.comBohdana Anna Dovhaliukprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):307</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2042Response of Cardiovascular System to the Effect of Cold in Individuals With Different Levels of Blood Pressure2025-04-06T22:14:47+02:00Petro Tabastabas@tdmu.edu.ua<p><em>Probl Cryobiol Cryomed 2024; 34(4):308</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2043Application of Cold to Diagnose the Functional State of Vestibular Analyzer2025-04-06T22:18:47+02:00Roman Shmataroman@tdmu.edu.ua<p><em>Probl Cryobiol Cryomed 2024; 34(4):308</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2044Thermosensitivity of Young People in Conditions of Extreme Climate Changes2025-04-06T22:23:12+02:00Viktoriia Hukhuk_vo@tdmu.edu.ua<p><em>Probl Cryobiol Cryomed 2024; 34(4):309</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2045Effect of Low Temperature Storage on Sensitivity to Antibiotics of Polyresistant Escherichia coli Strains2025-04-06T22:27:07+02:00Olena Peretyatkoelenageorz@gmail.comTetiana Gurinaprobl.cryobiol.cryomed@gmail.comYulya Yagnuk probl.cryobiol.cryomed@gmail.comSvitlana Krestetskaprobl.cryobiol.cryomed@gmail.comNadia Sklyarprobl.cryobiol.cryomed@gmail.comАndrii Yagnukprobl.cryobiol.cryomed@gmail.comOleksandr Pakhomovprobl.cryobiol.cryomed@gmail.comHalyna Bolshakovaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):309</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2046Viability of Escherichia coli Strains After Long Term Storage in Cryoprotective Media2025-04-06T22:45:14+02:00Yulya Yagnukelenageorz@gmail.comTetiana Gurinaprobl.cryobiol.cryomed@gmail.comSvitlana Krestetskaprobl.cryobiol.cryomed@gmail.comOlena Peretyatkoelenageorz@gmail.comNadia Sklyarprobl.cryobiol.cryomed@gmail.comАndrii Yagnukprobl.cryobiol.cryomed@gmail.comOleksandr Pakhomovprobl.cryobiol.cryomed@gmail.comHalyna Bolshakovaprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):310</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2047Influence of Nucleated Cells of Human Cord Blood on Indices of Immune System of Animals with Atopic Dermatitis2025-04-06T23:04:19+02:00Anatoliy Goltsevprobl.cryobiol.cryomed@gmail.comHanna Kovalprobl.cryobiol.cryomed@gmail.comMykola Bondarovychprobl.cryobiol.cryomed@gmail.comOlena Lutsenkoprobl.cryobiol.cryomed@gmail.comMaksym Ostankovprobl.cryobiol.cryomed@gmail.com<p><em>Probl Cryobiol Cryomed 2024; 34(4):310</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1997Cover of the Issue2025-04-04T18:10:17+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua2025-04-04T00:00:00+02:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1999Editorial Information2025-04-04T18:20:10+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua2025-04-04T00:00:00+02:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2000Content2025-04-04T18:25:09+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl Cryobiol Cryomed 2024; 34(4):250</em></p>2025-04-04T00:00:00+02:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2048Backmatter2025-04-07T09:00:21+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2049Key Word Index Volume. Vol. 342025-04-07T09:05:08+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl</em> <em>Cryobiol</em> <em>Cryomed</em><em> 2024; 34(4):314–316</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2050Table of Contents Vol. 342025-04-07T09:09:40+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl</em> <em>Cryobiol</em> <em>Cryomed</em><em> 2024; 34(4):321–324</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2051Instruction for Authors2025-04-07T09:14:03+02:00Editorial Board Problems of Cryobiology and Cryomedicinejournal@cryo.org.ua<p><em>Probl Cryobiol Cryomed 2024; 34(4):327-328</em></p>2024-12-26T00:00:00+01:00Copyright (c) 2025