Problems of Cryobiology and Cryomedicine
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed
<p>The Journal was established in 1985. Till 1991 it was published under title Kriobiologia, and Problemy kriobiologii/Problems of Cryobiology from 1991 till 2012. (<a href="/journal/index.php/probl-cryobiol-cryomed/at_a_glance">Journal at a Glance</a>)</p> <p>The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine.</p> <p>The Editorial Board takes the responsibility to provide a comprehensive peer-review of all submitted manuscripts. The Journal team strictly follows publishing ethics and standards to ensure high quality of scholarly publications. The Journal endorses the International Committee of Medical Journal Editors (ICMJE) <a href="http://www.icmje.org/recommendations/" target="_self">Recommendations for the Conduct, Reporting, Editing and Publication of Scholarly Work in Medical Journals</a>. (<a href="/journal/index.php/probl-cryobiol-cryomed/pubethics">Read more about Publishing Policy and Ethics</a>).</p> <p>Members of our Editorial Board are the specialists in various fields of low temperature biology and medicine from Ukraine and all over the world. (<a href="https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/about/editorialTeam">Editorial Board</a>).</p> <p>We do not charge the authors for article submission and/or processing and do not provide off-prints. The operation of the Journal is supported by National Academy of Sciences of Ukraine.</p> <p>Articles published in Problems of Cryobiology and Cryomedicine are Open-Access articles distributed under the terms and conditions of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/4.0/">CC BY</a>). We provide immediate open access to its content adhering to the principle that making research freely available to the public supports a greater global exchange of knowledge, increase visibility and readership. Use and distribution of the publications are permitted, retaining the credits to the authors and the Journal. The Journal holds the exclusive license to be the first publisher of the articles in print form and on-going non-exclusive license to disseminate the published content in various ways. The published version of the article could be deposited by authors to institutional or public repositories immediately on publication, stating a link to the URL of the published article on the journal's web-site.</p> <p>Our Journal is indexed/abstracted in <a href="http://www.scopus.com">Scopus</a>, <a href="http://thomsonreuters.com/en/products-services/scholarly-scientific-research/scholarly-search-and-discovery/zoological-record.html">Zoological Records</a>, <a href="http://www.cas.org/">Chemical Abstracts</a>, <a href="https://www.ebscohost.com/discovery?_ga=2.208532498.1058102411.1521647075-1463563202.1521647075">EBSCOhost</a>, <a href="http://bd.viniti.ru/">VINITI</a>, <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis64r_81/cgiirbis_64.exe?C21COM=F&I21DBN=REF_EX&P21DBN=REF&S21CNR=20&Z21ID=">Dzherelo </a>(Ukrainian Scientific Abstracts) and deposited in <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?Z21ID=&I21DBN=UJRN&P21DBN=UJRN&S21STN=1&S21REF=10&S21FMT=juu_all&C21COM=S&S21CNR=20&S21P01=0&S21P02=0&S21P03=PREF=&S21COLORTERMS=0&S21STR=KrioBiol">Vernadsky National Library of Ukraine Repository</a> (2002-now) and Google Books (<a href="https://books.google.co.jp/books?id=CSfuSZsuprkC&dq=kriobiologiya&hl=uk&source=gbs_all_issues_r&cad=1&atm_aiy=1970#all_issues_anchor">1975-1990</a>; <a href="https://books.google.com.ua/books?id=B326AAAAIAAJ&dq=problems+of+cryobiology&hl=uk&source=gbs_navlinks_s">1990-2002</a>).</p> <div id="gtx-anchor" style="position: absolute; visibility: hidden; left: 1473.54px; top: 296.181px; width: 9.88892px; height: 18.8889px;"> </div> <div class="jfk-bubble gtx-bubble" style="visibility: visible; left: 575px; top: 325px; opacity: 1;" role="alertdialog" aria-describedby="bubble-2"> <div id="bubble-2" class="jfk-bubble-content-id"> <div id="gtx-host" style="min-width: 200px; max-width: 400px;"> </div> </div> <div class="jfk-bubble-closebtn-id jfk-bubble-closebtn" tabindex="0" role="button" aria-label="Close"> </div> <div class="jfk-bubble-arrow-id jfk-bubble-arrow jfk-bubble-arrowup" style="left: 1470.56px;"> <div class="jfk-bubble-arrowimplbefore"> </div> <div class="jfk-bubble-arrowimplafter"> </div> </div> </div>Institute for Problems of Cryobiology and Cryomedicineen-USProblems of Cryobiology and Cryomedicine2307-6143<p>Authors who publish with this journal agree to the following terms:</p> <ul> <li class="show">Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a <a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.</li> </ul> <ul> <li class="show">Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.</li> </ul> <ul> <li class="show">Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See <a href="http://opcit.eprints.org/oacitation-biblio.html" target="_new">The Effect of Open Access</a>).</li> </ul>Modern Approaches and Perspectives of Human Cord Blood Nucleated Cells’ Freeze-Drying
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1915
<p>The review summarizes data on the prospects of using lyophilization as a methodical approach to preserve structure and functions for a long-term storage of human cord blood leukoconcentrate (HCBL). The advantages of the freeze-drying compared to low-temperature storage, natural or thermal drying have been shown. The question of the impact of physical and chemical factors, which are implemented at the stage of lyophilization, on structure and functions of HCBL cells has been considered. The importance of such factors as cell concentration, storage conditions, residual moisture in ensuring the preservation of lyophilized material was disputed. The paper analyzes the data on the use of lyoprotectants and antioxidants during freeze-drying as well as discusses the safety of using lyoprotectant delivery methods in relation to the genomic profile of cells.</p> <p> </p> <p><em>Probl Cryobiol Cryomed 2023; 33(</em><em>4</em><em>):</em> <em>227–249</em></p>Anatoliy GoltsevOlena LutsenkoLyudmyla OstankovaMykola BondarovychMaksym OstankovLarysa SokilIgor HrishaLyudmyla Chernyshenko
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2024-03-102024-03-10334227–249227–24910.15407/cryo33.04.227Study of Release of Biologically Active Compounds from Cord Blood Under Different Conditions of Low-Temperature Impact
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1917
<p>Here, we have studied the impact of cord blood destruction method on composition of the cord blood-derived low molecular fractions, and compared the cryodestruction with other methods of cell destruction before extracting. Human cord blood was destroyed by rapid or slow freezing / warming, hypotonic lysis and thermal destruction. The obtained substance was used to produce the cord blood fraction (CBF) by multi-stage ultrafiltration and lyophilization. Dry weight, CBF composition and total protein content in them were evaluated by chromatographic profiles (gel permeation and reverse-phase high-performance liquid chromatography). The CBFs, obtained by different techniques for cord blood destruction were established to differ in the content and molecular weights of the components. These findings suggest the possibility to vary the amount and range of low molecular weight compounds in lyophilized cord blood fractions by using low temperatures and combining different regimens of freezing / warming.</p> <p> </p> <p><em>Probl Cryobiol Cryomed 2023; 33(4):</em> <em>250–262</em></p>Nataliia MoisieievaIvan ShcheniavskyiOlga GorinaYuliia AkhatovaOleksandr Semenchenko
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2023-12-302023-12-30334250–262250–26210.15407/cryo33.04.250Low Temperature Influence on Activity in Alginate Microcapsules Incorporated-Enzymes
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1918
<p><em>Probl Cryobiol Cryomed 2023; 33(4): 263–267</em></p>Iryna KhalaKateryna RozanovaSvitlana RepinaOleg Nardid
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2023-12-302023-12-30334263–267263–26710.15407/cryo33.04.263Changes in Blood Leukocyte Composition in Rats of Different Ages after Long-term Cold Adaptation
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1919
<p><em>Probl Cryobiol Cryomed 2023; 33(4): 268–273</em></p>Viktoria Lomako
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2023-12-302023-12-30334268–273268–27310.15407/cryo33.04.268Cryopreserved Placental Derivatives Improve Quality of Life and Behavioral Responses in Mice with Chemotherapy-Induced Ovarian Failure
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1920
<p><em>Probl Cryobiol Cryomed 2023; 33(4): 274–279</em></p>Mariіa ShevchenkoTetyana MykhalchukOlga ProkopіukVolodymyr KarpenkoVolodymyr Prokopіuk
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2023-12-302023-12-30334274–279274–27910.15407/cryo33.04.27490th birthday of Prof. Tetiana M. Yurchenko
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1921
<p><em>Probl</em> <em>Cryobiol</em> <em>Cryomed</em><em> 2023; 33(4):</em><em>296-297</em></p>Editorial Board Problems of Cryobiology and Cryomedicine
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2023-12-302023-12-30334296297Abstracts of the 47th Annual Conference of Young Scientists ‘Cold in Biology and Medicine. Current Issues in Cryobiology, Transplantology and Biotechnology’ May 23, 2023, Kharkiv, Ukraine
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1922
<p><em>Probl Cryobiol Cryomed 2023; 33(4):280-282</em></p>Editorial Board Problems of Cryobiology and Cryomedicine
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2023-12-302023-12-30334280282The Effect of Varying Thawing Temperatures and Preserving Conditions on the Recovery Rate of Cryopreserved Equine Dendritic Cells
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1923
<p><em>Probl Cryobiol Cryomed 2023; 33(4):283</em></p>Tarek DeebFelix SchwebelTim RittinghausBirgit Glasmacher
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2023-12-302023-12-3033428328310.15407/cryo33.04.283Hatching of Cryopreserved Human Blastocysts Obtained in IVF and ICSI Cycles
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1924
<p><em>Probl Cryobiol Cryomed 2023; 33(4):283</em></p>Nataliia ShapovalovaTaisiia YurchukVolodymyr PinyaevMaryna Petrushko
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2023-12-302023-12-3033428328310.15407/cryo33.04.283aCorrelation Between Biochemical Parameters of Seminal Plasma and Cryoresistance of Spermatozoa of Saanen Bucks
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1925
<p><em>Probl Cryobiol Cryomed 2023; 33(4):284</em></p>Anastasiia BogdaniukMaryna PetrushkoVolodymyr Garkavii
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2023-12-302023-12-3033428428410.15407/cryo33.04.284Potential Ways for Fish Biodiversity Conservation Within Siversky Donets Basin in Ukraine Using Cryobiology and Cryobanking Techniques
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1926
<p><em>Probl Cryobiol Cryomed 2023; 33(4):284</em></p>Daniil OstrasHennadiy HoncharovKostiantyn Mikson
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2023-12-302023-12-3033428428410.15407/cryo33.04.284aPreservation of Human Mesenchymal Stromal Cells in Protein-Supplemented Alginate Capsules
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1927
<p><em>Probl Cryobiol Cryomed 2023; 33(4):285</em></p>Oleksandra HubeniaSara Leal MarinNatalia Trufanova
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2023-12-302023-12-3033428528510.15407/cryo33.04.285Сryoresistance of Autotrophic Organisms From Polar Regions Sensed by Chlorophyll Fluorescence Method
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1928
<p><em>Probl Cryobiol Cryomed 2023; 33(4):285</em></p>Davide GiordanoAnton PuhovkinJosef HájekJiří SekerákMiloš Bartak
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2023-12-302023-12-3033428528510.15407/cryo33.04.285aDevelopment of a Numerical Simulation Model for Continuous Floating Cryopreservation of Red Blood Cell Concentrate
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1929
<p><em>Probl Cryobiol Cryomed 2023; 33(4):286</em></p>Lisa KamphausDiaa KhayyatRobin KahlfeldJanina HagedornStephan KabelacBirgit Glasmacher
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2023-12-302023-12-3033428628610.15407/cryo33.04.286Impact of Environmental Factors on the Moss Banks’ State in Argentine Islands (West Antarctica): Interannual Monitoring
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1930
<p><em>Probl</em> <em>Cryobiol</em> <em>Cryomed</em><em> 2023; 33(4):286</em></p>Anton PuhovkinHanna YevchunMilos BartakJosef HajekYuri ShepetaNatalia MiryutaIvan Parnikoza
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2023-12-302023-12-3033428628610.15407/cryo33.04.286aDiscriminating Cells by Membrane Surface Charge May Improve Long-Term Refrigerated Storage of Red Blood Cells
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1931
<p><em>Probl Cryobiol Cryomed 2023; 33(4):287</em></p>Daniil LiadovVolodymyr Berest
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2023-12-302023-12-3033428728710.15407/cryo33.04.287Software Solution for Moving Objects Identification and Tracking Individual Behavior in Studies of Fish Adaptation for Sub-Ambient Temperatures
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1932
<p><em>Probl Cryobiol Cryomed 2023; 33(4):287</em></p>Polina Berest
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2023-12-302023-12-3033428728710.15407/cryo33.04.287aFeatures of Formation and Cryopreservation of Spheroids From Brain Cells of Newborn Rats
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1933
<p><em>Probl Cryobiol Cryomed 2023; 33(4):288</em></p>Svitlana VsevolodskaOleksandr SukachOlga Ochenashko
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2023-12-302023-12-3033428828810.15407/cryo33.04.288Effect of Low Temperatures on Morphology of Alginate Microcarriers
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1934
<p><em>Probl Cryobiol Cryomed 2023; 33(4):288</em></p>Stanislav NarozhnyiOlena BobrovaIgor Kovalenko
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2023-12-302023-12-3033428828810.15407/cryo33.04.288aComparative Analysis of Sensitivity of Canine and Human Erythrocytes to Changes in Osmotic Conditions of the Medium
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1935
<p><em>Probl Cryobiol Cryomed 2023; 33(4):289</em></p>Yevhenii HubeniaSerhii YershovOlena ChabanenkoNataliia Shpakova
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2023-12-302023-12-3033428928910.15407/cryo33.04.289The Effect of Salinity on the Resistance of Plant Cells to Low and Ultra-Low Temperatures on the Example of Dunaliella salina Microalgae Cells
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1936
<p><em>Probl Cryobiol Cryomed 2023; 33(4):289</em></p>Nadiia ChernobaiKrystyna Vozovyk
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2023-12-302023-12-3033428928910.15407/cryo33.04.289aCytotoxicity and Cryoprotective Efficiency of Polyvinyl Alcohol Solutions During Freezing of Human Erythrocytes
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1937
<p><em>Probl Cryobiol Cryomed 2023; 33(4):290</em></p>Yana HvozdiukYulia PakhomovaValentina ChekanovaAntonina Kompaniets
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2023-12-302023-12-3033429029010.15407/cryo33.04.290Optimization of Methods for Obtaining and Evaluating Organotypic Liver Cultures for Further Cryopreservation
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1938
<p><em>Probl Cryobiol Cryomed 2023; 33(4):290</em></p>Аnna KaverinskaVolodymyr Prokopiuk
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2023-12-302023-12-3033429029010.15407/cryo33.04.290aPeculiarities of the Structure of Frozen Cryoprotective Solutions
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1939
<p><em>Probl Cryobiol Cryomed 2023; 33(4):291</em></p>Stanislav SevastianovOleksandr Osetsky
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2023-12-302023-12-3033429129110.15407/cryo33.04.291Paclitaxel Induces Axonal Degeneration and Alters Cold Temperature Sensitivity in Adult Rats
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1940
<p><em>Probl Cryobiol Cryomed 2023; 33(4):291</em></p>Zuzana MichalováIvo Vanický
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2023-12-302023-12-3033429129110.15407/cryo33.04.291aMorphofunctional Feature of the Formed Elements of Blood Cells and the Central Lymphoid Organ Under Conditions of Short-Term Hypothermic Exposure
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1941
<p><em>Probl Cryobiol Cryomed 2023; 33(4):292</em></p>Ruslan Aminov
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2023-12-302023-12-3033429229210.15407/cryo33.04.292Characterization of the Hepatoprotective Activity of Placenta Cryoextract in Liver Lesions of Various Etiologies
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1942
<p><em>Probl Cryobiol Cryomed 2023; 33(4):292</em></p>Illia KoshurbaMykola ChyzhFedir Hladkykh
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2023-12-302023-12-3033429229210.15407/cryo33.04.292aFipronil Impairs Proliferation in the Olfactory Neurogenic Region and Alters Low Temperature Sensitivity in Adult Mice
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1944
<p><em>Probl Cryobiol Cryomed 2023; 33(4):293</em></p>Kamila FabianováJozef PiskoDušan FabianMarcela MartončíkováAdam RačekEnikő Račeková
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2023-12-302023-12-3033429329310.15407/cryo33.04.293Effect of Medium Conditioned by Cryopreserved Placental Cells on Organotypic Ovarian Culture
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1945
<p><em>Probl Cryobiol Cryomed 2023; 33(4):293</em></p>Tetyana MykhalchukVolodymyr ProkopiukMariia ShevchenkoOlga Prokopiuk
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2023-12-302023-12-3033429329310.15407/cryo33.04.293aElectrospun Scaffolds for the Hypothermic Preservation of Cells
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1946
<p><em>Probl Cryobiol Cryomed 2023; 33(4):294</em></p>Sven-Alexander BarkerSara Leal MarinYvonne RogerAndrea HoffmannEyal ZussmanBirgit Glasmacher
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2023-12-302023-12-3033429429410.15407/cryo33.04.294Long-Term Results of Cryosurgery and Combined Treatment of Patients With Deep Brain Tumors
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1947
<p><em> </em></p> <p><em>Probl Cryobiol Cryomed 2023; 33(4):294</em></p>Vladyslav KaliuzhkaVolodymyr PiatykopOleksandra Cherniaieva
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2023-12-302023-12-3033429429410.15407/cryo33.04.294aProspects for the Use of Cryopreserved Bone Marrow Mesenchymal Stem Cells in the Treatment of Neurological Diseases and Injuries
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1948
<p><em>Probl Cryobiol Cryomed 2023; 33(4):295</em></p>Vladyslav KaliuzhkaVolodymyr PiatykopOlena ShchegelskaOleksandra Cherniaieva
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2023-12-302023-12-3033429529510.15407/cryo33.04.295Development of Electrodes for Cryopreservation of Stem Cells by Electroporation-Assisted Loading with Sucrose
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1949
<p><em>Probl Cryobiol Cryomed 2023; 33(4):295</em></p>Anna SchwäbleinDiaa KhayyatSara Leal MarinBirgit Glasmacher
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2023-12-302023-12-3033429529510.15407/cryo33.04.295aCover of the Issue
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1912
Editorial Board Problems of Cryobiology and Cryomedicine
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2024-03-102024-03-10334Editorial Information
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1913
Editorial Board Problems of Cryobiology and Cryomedicine
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2024-03-102024-03-10334Table of Contents
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1914
<p><em>Probl Cryobiol Cryomed 2023; 33(4):226</em></p>Editorial Board Problems of Cryobiology and Cryomedicine
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2024-03-102024-03-10334225226Key Word Index Vol. 33
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1950
<p><em>Probl Cryobiol Cryomed 2023; 32(4):301–303</em></p>Editorial Board Problems of Cryobiology and Cryomedicine
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2023-12-302023-12-30334298303Table of Contents Vol. 33
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1951
<p><em>Probl Cryobiol Cryomed 2023; 33(4):3</em><em>08</em><em>–3</em><em>11</em></p>Editorial Board Problems of Cryobiology and Cryomedicine
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2023-12-302023-12-30334304311Instruction for Authors
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1952
<p><em>Probl Cryobiol Cryomed 2023; 33(4):314-315</em></p>Editorial Board Problems of Cryobiology and Cryomedicine
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2023-12-302023-12-30334312315Backmatter
http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1953
<p><em>Probl Cryobiol Cryomed 2023; 33(4):316</em></p>Editorial Board Problems of Cryobiology and Cryomedicine
Copyright (c) 2024
https://creativecommons.org/licenses/by/4.0
2023-12-302023-12-30334316316