http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed <p>The Journal was established in 1985. Till 1991 it was published under title Kriobiologia, and Problemy kriobiologii/Problems of Cryobiology from 1991 till 2012. (<a href="/journal/index.php/probl-cryobiol-cryomed/at_a_glance">Journal at a Glance</a>)</p> <p>The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine.</p> <p>The Editorial Board takes the responsibility to provide a comprehensive peer-review of all submitted manuscripts. The Journal team strictly follows publishing ethics and standards to ensure high quality of scholarly publications. The Journal endorses the International Committee of Medical Journal Editors (ICMJE) <a href="http://www.icmje.org/recommendations/" target="_self">Recommendations for the Conduct, Reporting, Editing and Publication of Scholarly Work in Medical Journals</a>. (<a href="/journal/index.php/probl-cryobiol-cryomed/pubethics">Read more about Publishing Policy and Ethics</a>).</p> <p>Members of our Editorial Board are the specialists in various fields of low temperature biology and medicine from Ukraine and all over the world. (<a href="https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/about/editorialTeam">Editorial Board</a>).</p> <p>We do not charge the authors for article submission and/or processing and do not provide off-prints. The operation of the Journal is supported by National Academy of Sciences of Ukraine.</p> <p>Articles published in Problems of Cryobiology and Cryomedicine are Open-Access articles distributed under the terms and conditions of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/4.0/">CC BY</a>). We provide immediate open access to its content adhering to the principle that making research freely available to the public supports a greater global exchange of knowledge, increase visibility and readership. Use and distribution of the publications are permitted, retaining the credits to the authors and the Journal. The Journal holds the exclusive license to be the first publisher of the articles in print form and on-going non-exclusive license to disseminate the published content in various ways. The published version of the article could be deposited by authors to institutional or public repositories immediately on publication, stating a link to the URL of the published article on the journal's web-site.</p> <p>Our Journal is indexed/abstracted in <a href="http://www.scopus.com">Scopus</a>, <a href="http://thomsonreuters.com/en/products-services/scholarly-scientific-research/scholarly-search-and-discovery/zoological-record.html">Zoological Records</a>, <a href="http://www.cas.org/">Chemical Abstracts</a>, <a href="https://www.ebscohost.com/discovery?_ga=2.208532498.1058102411.1521647075-1463563202.1521647075">EBSCOhost</a>,&nbsp;<a href="http://bd.viniti.ru/">VINITI</a>, <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis64r_81/cgiirbis_64.exe?C21COM=F&amp;I21DBN=REF_EX&amp;P21DBN=REF&amp;S21CNR=20&amp;Z21ID=">Dzherelo </a>(Ukrainian Scientific Abstracts) and deposited in <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?Z21ID=&amp;I21DBN=UJRN&amp;P21DBN=UJRN&amp;S21STN=1&amp;S21REF=10&amp;S21FMT=juu_all&amp;C21COM=S&amp;S21CNR=20&amp;S21P01=0&amp;S21P02=0&amp;S21P03=PREF=&amp;S21COLORTERMS=0&amp;S21STR=KrioBiol">Vernadsky National Library of Ukraine Repository</a> (2002-now) and Google Books (<a href="https://books.google.co.jp/books?id=CSfuSZsuprkC&amp;dq=kriobiologiya&amp;hl=uk&amp;source=gbs_all_issues_r&amp;cad=1&amp;atm_aiy=1970#all_issues_anchor">1975-1990</a>; <a href="https://books.google.com.ua/books?id=B326AAAAIAAJ&amp;dq=problems+of+cryobiology&amp;hl=uk&amp;source=gbs_navlinks_s">1990-2002</a>).</p> <div id="gtx-anchor" style="position: absolute; visibility: hidden; left: 1473.54px; top: 296.181px; width: 9.88892px; height: 18.8889px;">&nbsp;</div> <div class="jfk-bubble gtx-bubble" style="visibility: visible; left: 575px; top: 325px; opacity: 1;" role="alertdialog" aria-describedby="bubble-2"> <div id="bubble-2" class="jfk-bubble-content-id"> <div id="gtx-host" style="min-width: 200px; max-width: 400px;">&nbsp;</div> </div> <div class="jfk-bubble-closebtn-id jfk-bubble-closebtn" tabindex="0" role="button" aria-label="Close">&nbsp;</div> <div class="jfk-bubble-arrow-id jfk-bubble-arrow jfk-bubble-arrowup" style="left: 1470.56px;"> <div class="jfk-bubble-arrowimplbefore">&nbsp;</div> <div class="jfk-bubble-arrowimplafter">&nbsp;</div> </div> </div> Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine en-US Problems of Cryobiology and Cryomedicine 2307-6143 <p>Authors who publish with this journal agree to the following terms:</p> <ul> <li class="show">Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a <a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.</li> </ul> <ul> <li class="show">Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.</li> </ul> <ul> <li class="show">Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See <a href="http://opcit.eprints.org/oacitation-biblio.html" target="_new">The Effect of Open Access</a>).</li> </ul> http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2164 Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2166 Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2167 <p><em>Probl Cryobiol Cryomed 2025; 35(4): 165-166</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 165 166 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2111 <p>Cryopreservation of cell suspensions, such as testicular interstitial cells (ICs), which include cells differing in size, volume, and membrane composition, is a very challenging task. Cryobiology requires the determination of several parameters to optimize the cryopreservation procedure, such as membrane water permeability (or hydraulic conductivity) (Lp) and its activation energy (Ea0), the permeability of the cell plasma membrane to dimethyl sulfoxide (DMSO) (Ps) and its activation energy (Ea1), and the osmotically inactive cell volume. This study aims to measure these key cryopreservation parameters for ICs. Additionally, it seeks to evaluate how quickly ICs become saturated with DMSO and to determine the optimal exposure time to cryoprotective media before cooling. By analyzing cell volume changes in different DMSO-based solutions, the research intends to optimize the cryopreservation process, potentially eliminating unnecessary exposure steps while maintaining cell viability. The kinetics of ICs volume changes in cryoprotective media with different DMSO concentrations (0.7—2.8 M), which additionally included 100 mg/ml dextran 40 (0.7DMSO + D40) or 10% FBS (1.4DMSO + FBS), were analyzed using the modified Kedem–Katchalsky model to determine the membrane transport properties. The calculated parameters clearly showed that cell saturation with DMSO occurred within a few minutes after its addition and could reach equilibrium before the estimated start of water crystallization in the samples. Thus, additional exposure of cells to DMSO-containing media was unnecessary. It could potentially lower the metabolic activity of ICs. Accordingly, the cryopreservation procedure for ICs can be shortened by removing the exposure step without reducing viability of ICs in 0.7DMSO + D40 and 1.4DMSO + FBS.</p> <p><em>Probl Cryobiol Cryomed. 2025; 35(4): 194—207</em></p> <p>&nbsp;</p> Oleksandr Pakhomov Igor Kovalenko Nadiia Chernobai Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2026-03-08 2026-03-08 35 4 196—209 196—209 10.15407/cryo35.04.196 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2112 <p>The research performed a local histological assessment of tissue tolerance of a solution containing hyaluronic acid, as well as its effect on the course of the inflammatory process aft er low-temperature skin damage was investigated. The safety of using a 1% solution of high molecular weight hyaluronic acid with the addition of 5% dimethyl sulfoxide (DMSO) and its effect on the inflammation development was studied using a model of low-temperature tissue injury based on the results of clinical observation, planimetric and histological studies. Additionally, the effect of 1% solutions of high molecular weight (&gt; 2,000 kDa) and low molecular weight (10—100 kDa) hyaluronic acid on the inflammatory process aft er low-temperature tissue injury was comparatively explored. According to the results of the histological study, no morphological signs of toxic or other negative effects of subcutaneous administration of a 1% solution of high molecular weight hyaluronic acid with the addition of 5% DMSO on tissues were found. Comparison of the effects of cryoprotective solutions on the course of the wound process after low-temperature tissue damage revealed the differences both by the severity of macroscopic signs of the inflammation and by planimetric study data. The inclusion of hyaluronic acid in the composition of the cryoprotective solution is considered a optimistic approach to reducing the concentration of DMSO in order to create solutions that can be used without a washing-out stage.</p> <p>&nbsp;</p> <p><em>Probl Cryobiol Cryomed. 2025; 35(4): 208—218</em></p> Tetiana Gurina Gennadiy Kovalov Hanna Poliakova Olga Naumova Volodymyr Markovsky Anatolii Seliuta Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2026-03-08 2026-03-08 35 4 210—220 210—220 10.15407/cryo35.04.210 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2113 <p>Despite increasing interest to the role of hypoxia in reproductive processes, its impact on sperm capacitation and cryoresistance remains insufficiently described. This study investigated the effect of oxygen tension during pre-cryopreservation sperm incubation on the capacitation efficiency and examined the relationship between capacitation and cryoresistance. According to the WHO criteria, ejaculates from 30 healthy normozoospermic men were analyzed. Functional characteristics and cryoresistance of spermatozoa were assessed after density gradient processing and <em>in vitro</em> capacitation under 1, 5 and 21% oxygen concentrations. An incubation of human spermatozoa under moderate hypoxia was found to positively influence the viability, motility, and capacitation activation. For the first time, we have demonstrated that oxygen tension during <em>in vitro</em> incubation is a critical factor determining sperm survival following thawing. These findings suggest that moderate hypoxia may represent a promising biotechnological strategy to improve sperm cryopreservation protocols and enhance the efficiency of assisted reproductive technologies.</p> <p>&nbsp;</p> <p><em>Probl Cryobiol Cryomed. 2025; 35(4): 219—223</em></p> Maryna Petrushko Taisiia Yurchuk Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2026-03-08 2026-03-08 35 4 221—225 221—225 10.15407/cryo35.04.221 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2114 <p><em>Probl Cryobiol Cryomed. 2025; 35(4): 224—226</em></p> Ivan Shcheniavskyi Yuliia Akhatova Natalia Moisieieva Olga Gorina Oksana Lipko Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2026-03-08 2026-03-08 35 4 226—228 226—228 10.15407/cryo35.04.226 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2115 <p><em>Probl Cryobiol Cryomed. 2025; 35(4): 229</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 229 229 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2116 <p><em>Probl Cryobiol Cryomed. 2025; 35(4): 230</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 230 230 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2168 Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2169 <p><em>Probl Cryobiol Cryomed 2025; 35(4): 253-254</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 253 254 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2170 <p><em>Probl Cryobiol Cryomed 2025; 35(4):25</em><em>5</em><em>-25</em><em>6</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 255 256 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2171 <p><em>Probl Cryobiol Cryomed 2025; 35(4):25</em><em>7</em><em>-25</em><em>8</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 257 258 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2117 <p><em>Probl Cryobiol Cryomed 2025; 35(4):231</em></p> Volodymyr Nahrebetskyi Fedir Prytkov Oksana Tsurikova Nikolai Korpan Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 231 231 10.15407/cryo35.04.231 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2118 <p><em>Probl Cryobiol Cryomed 2025; 35(4):231</em></p> Ruslana Obushko Natalia Trufanova Olena Revenko Oleksandr Petrenko Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 229 229 10.15407/cryo35.04.231a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2119 <p><em>Probl Cryobiol Cryomed 2025; 35(4):232</em></p> Tarek Deeb Felix Schwebel Christina Winkler Claudia Dettmer-Richardt Dierk Rebeski Birgit Glasmacher Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 232 232 10.15407/cryo35.04.232 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2120 <p><em>Probl Cryobiol Cryomed 2025; 35(4):232</em></p> Ramiro González Francisco Rocha Estefanía Paredes Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 232 232 10.15407/cryo35.04.232a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2121 <p><em>Probl Cryobiol Cryomed 2025; 35(4):233</em></p> Lora Gaydarova Elena Hristova Plamen Todorov Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 233 233 10.15407/cryo35.04.233 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2122 <p><em>Probl Cryobiol Cryomed 2025; 35(4):233</em></p> Adrian Meis-Solla Anxo Iglesias Jose de Santiago-Meiide Jesús Souza Troncoso Estefania Paredes Rosendo Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 233 233 10.15407/cryo35.04.233a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2123 <p><em>Probl Cryobiol Cryomed 2025; 35(4):234</em></p> Jiří Sekerák Jr. Josef Hájek Miloš Barták Jiří Zámečník Miloš Faltus Anton Puhovkin Ivan Parnikoza Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 234 234 10.15407/cryo35.04.234 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2124 <p><em>Probl Cryobiol Cryomed 2025; 35(4):234</em></p> Olga Maiorova Oleksandr Sukach Olga Ochenashko Svitlana Vsevolodska Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 234 234 10.15407/cryo35.04.234a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2125 <p><em>Probl Cryobiol Cryomed 2025; 35(4):235</em></p> Oleksandra Hubenia Sara Leal-Marin Natalia Trufanova Birgit Glasmacher Oleksandr Petrenko Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 235 235 10.15407/cryo35.04.235 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2126 <p><em>Probl Cryobiol Cryomed 2025; 35(4):235</em></p> Emmerson Teas Kate Fowler Nilay Kuscu Peter Kilbride Suzannah Williams Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 235 235 10.15407/cryo35.04.235a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2127 <p><em>Probl Cryobiol Cryomed 2025; 35(4):236</em></p> Anastasiia Bogdaniuk Tom Otter Maryna Petrushko Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 236 236 10.15407/cryo35.04.236 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2128 <p><em>Probl Cryobiol Cryomed 2025; 35(4):236</em></p> Saskia Altmaier Frank Stracke Ina Meiser Julia Neubauer Heiko Zimmermann Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 236 236 10.15407/cryo35.04.236a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2129 <p><em>Probl Cryobiol Cryomed 2025; 35(4):237</em></p> Lina Konoplia Anna Feskova Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 237 237 10.15407/cryo35.04.237 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2130 <p><em>Probl Cryobiol Cryomed 2025; 35(4):237</em></p> Oleksandra Hubenia Rouven Tewes Sara Leal-Marin Christina Winkler Abdul-Rashid Iddi Birgit Glasmacher Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 237 237 10.15407/cryo35.04.237a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2131 <p><em>Probl Cryobiol Cryomed 2025; 35(4):238</em></p> Abdul-Rashid Iddi Merve Sinram Sara Leal-Marin Christina Winkler Fabian Will Oleksandra Hubenia Rouven Tewes Birgit Glasmacher Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 238 238 10.15407/cryo35.04.238 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2132 <p><em>Probl Cryobiol Cryomed 2025; 35(4):238</em></p> Rouven Tewes Birgit Glasmacher Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 238 238 10.15407/cryo35.04.238a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2133 <p><em>Probl Cryobiol Cryomed 2025; 35(4):239</em></p> Olena Chabanenko Natalia Yershova Nataliia Shpakova Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 239 239 10.15407/cryo35.04.239 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2134 <p><em>Probl Cryobiol Cryomed 2025; 35(4):239</em></p> Elnaz Zand Gang Zhao Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 239 239 10.15407/cryo35.04.239a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2135 <p><em>Probl Cryobiol Cryomed 2025; 35(4):240</em></p> Svitlana Vsevolodska Olga Ochenashko Olga Maiorova Oleksandr Sukaсh Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 240 240 10.15407/cryo35.04.240 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2136 <p><em>Probl Cryobiol Cryomed 2025; 35(4):240</em></p> Оlga Kuzmina Іryna Kuzmina Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 240 240 10.15407/cryo35.04.240a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2137 <p><em>Probl Cryobiol Cryomed 2025; 35(4):241</em></p> Anna Feskova Oleksandr Feskov Lina Konoplia Yevhenia Zhylkova Iryna Feskova Olena Blazhko Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 241 241 10.15407/cryo35.04.241 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2138 <p><em>Probl Cryobiol Cryomed 2025; 35(4):241</em></p> Halyna Kopylchuk Ivanna Nykolaichuk Mariia Ursatyi Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 241 241 10.15407/cryo35.04.241a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2139 <p><em>Probl Cryobiol Cryomed 2025; 35(4):242</em></p> Volodymyr Dzhyvak Nazarii Krulyk Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 242 242 10.15407/cryo35.04.242 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2140 <p><em>Probl Cryobiol Cryomed 2025; 35(4):242</em></p> Anatolii Seliuta Gennadiy Kovalov Hanna Poliakova Tetiana Gurina Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 242 242 10.15407/cryo35.04.242a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2141 <p><em>Probl Cryobiol Cryomed 2025; 35(4):243</em></p> Yevheniia Hromko Maryna Zhulikova Mykhailo Myroshnychenko Oksana Nakonechna Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 243 243 10.15407/cryo35.04.243 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2142 <p><em>Probl Cryobiol Cryomed 2025; 35(4):243</em></p> Yana Hvozdiuk Svitlana Kovalenko Tetiana Gurina Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 243 243 10.15407/cryo35.04.243a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2143 <p><em>Probl Cryobiol Cryomed 2025; 35(4):244</em></p> Vladyslav Mirnyy Nataliia Moisieieva Yuliia Akhatova Olha Gorina Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 244 244 10.15407/cryo35.04.244 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2144 <p><em>Probl Cryobiol Cryomed 2025; 35(4):244</em></p> Ihor Perepelitsa Volodymyr Berest Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 244 244 10.15407/cryo35.04.244a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2145 <p><em>Probl Cryobiol Cryomed 2025; 35(4):245</em></p> Olga Klymukhina Maryna Petrushko Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 245 245 10.15407/cryo35.04.245 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2146 <p><em>Probl Cryobiol Cryomed 2025; 35(4):245</em></p> Andrii Khomenko Kostiantyn Fomenko Valeriy Cherepanov Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 245 245 10.15407/cryo35.04.245a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2147 <p><em>Probl Cryobiol Cryomed 2025; 35(4):246</em></p> Oleksandr Zubkov Oleh Trufanov Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 246 246 10.15407/cryo35.04.246 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2148 <p><em>Probl Cryobiol Cryomed 2025; 35(4):246</em></p> Kostiantyn Fomenko Andrii Khomenko Daniil Ostras Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 246 246 10.15407/cryo35.04.246a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2149 <p><em>Probl Cryobiol Cryomed 2025; 35(4):247</em></p> Karyna Hrebeniuk Olha Denysova Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 247 247 10.15407/cryo35.04.247 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2150 <p><em>Probl Cryobiol Cryomed 2025; 35(4):247</em></p> Valeriia Dorofieieva Nataliia Kapustnyk Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 247 247 10.15407/cryo35.04.247a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2151 <p><em>Probl Cryobiol Cryomed 2025; 35(4):248</em></p> Igor Kandybko Larysao Syrotenko Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 248 248 10.15407/cryo35.04.248 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2152 <p><em>Probl Cryobiol Cryomed 2025; 35(4):248</em></p> Serhii Sidorovskiy Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 248 248 10.15407/cryo35.04.248a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2153 <p><em>Probl Cryobiol Cryomed 2025; 35(4):249</em></p> Mykhailo Pohribnyi Mykola Vinnikov Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 249 249 10.15407/cryo35.04.249 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2154 <p><em>Probl Cryobiol Cryomed 2025; 35(4):249</em></p> Irina Martyniuk Оleksandrv Akimov Anatoliy Paliy Оlena Pavlichenko Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 249 249 10.15407/cryo35.04.249a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2155 <p><em>Probl Cryobiol Cryomed 2025; 35(4):250</em></p> Dmytro Salnykov Anastasiya Skoryk Mariia Shevchenko Ivan Shcheniavskyi Oleg Chub Stanislav Voitenko Oleksandr Osetsky Olga Prokopiuk Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 250 250 10.15407/cryo35.04.250 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2156 <p><em>Probl Cryobiol Cryomed 2025; 35(4):250</em></p> Anna Dvinskykh Natalia Khokhlenkova Olha Kalyuzhna Nataliia Dvinskykh Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 250 250 10.15407/cryo35.04.250a http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2172 <p><em>Probl Cryobiol Cryomed 2025; 35(4):2</em><em>51</em><em>-25</em><em>2</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2025-12-30 2025-12-30 35 4 251 252 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2109 <p>The paper considers the legal and bioethical foundations of the activity of cryobanks of human cells and tissues in Ukraine. The importance of adapting national legislation to international standards in the field of biobanking, transplantology and assisted reproductive technologies is shown. The study provides an analysis of the basics of legal regulation of cryobanks, identifies directions for improving the legislation of Ukraine in accordance with European standards. For the analysis, the authors were guided by the laws of Ukraine, directives of the European Union, recommendations of the World Health Organization, documents of the Council of Europe, and used scientific publications in the field of medical law, bioethics, cryobiology, and reproductive medicine. Regulatory, documentary, comparative methods of analysis were applied. It has been established that the current regulatory framework for governing the activities of cryobanks is fragmented, does not determine the legal status of biological material, the procedure for its inheritance and use after the donor’s death. The proposed ways to improve the legal field are as follows: adoption of a special law on biobanks, creation of a unified state register of biological samples, adaptation of protocols and procedures to the norms of the European Union. The implementation of the proposed measures will contribute to the transparency, ethics, and efficiency of cryobanks, the protection of the rights of donors and patients, and the development of biomedical research in Ukraine.</p> <p><em>Probl Cryobiol Cryomed. 2025; 35(4): 165—178</em></p> Valentyn Shapovalov Oleksandr Nevzghoda Viktoriia Shapovalova Alina Osyntseva Valerii Shapovalov Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2026-03-08 2026-03-08 35 4 167—180 167—180 10.15407/cryo35.04.167 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2110 <p>Assessment of cell viability is critically important in the context of cryobiological research, as it allows determining the effectiveness of freeze-thawing procedures and low-temperature storage of isolated cells. The review systematizes the methods for determining cell viability used in cryobiological research, with an emphasis on their practical applicability, accuracy, and sensitivity. Both traditional methods based on the assessment of cell membrane integrity and metabolic activity, such as trypan blue staining, MTT assay, fluorescent dye assays, and more modern approaches, including flow cytometry, confocal microscopy, and methods for assessing cell functional activity, are reviewed. For each method, a description of the mechanism of action, a protocol, specific advantages, disadvantages, as well as equipment requirements and limitations for cell types are provided. A comparative analysis was conducted, which revealed the lack of a universal approach, but demonstrated the effectiveness of combined methods that combine fluorescent staining and metabolic assessment. Such combinations allow for high accuracy in assessing cell viability after cryopreservation. In addition, the review discusses the prospects for the development of methods for determining cell viability in cryobiology, including the introduction of the latest high-resolution imaging technologies, multiparametric analysis, as well as integration with microfluidic platforms and artificial intelligence to automate and improve the accuracy of the assessment. The review can serve as a practical tool for researchers in choosing the most optimal approach for assessing cell viability depending on the purpose of the experiment, available resources and cell type.</p> <p>&nbsp;</p> <p><em>Probl Cryobiol Cryomed. 2025; 35(4): 179—193&nbsp;&nbsp; </em></p> Oleksandr Sukach Olga Maiorova Copyright (c) 2026 https://creativecommons.org/licenses/by/4.0 2026-03-08 2026-03-08 35 4 181—195 181—195 10.15407/cryo35.04.181