Problems of Cryobiology and Cryomedicine http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed <p>The Journal was established in 1985. Till 1991 it was published under title Kriobiologia, and Problemy kriobiologii/Problems of Cryobiology from 1991 till 2012. (<a href="/journal/index.php/probl-cryobiol-cryomed/at_a_glance">Journal at a Glance</a>)</p> <p>The Journal publishes the reviews and original papers on cryobiological and cryomedical research, in particular the elucidation of mechanisms of injuries occurring in biological objects and caused by the influence of low and ultra low temperatures; natural resistance of biologicals to cold and their recovery post effect; the development of effective methods of cryoprotection and technology of storage of biological resources under hypothermic and ultra low temperatures, application of hypothermia, cryotherapy and cryopreserved biologicals for treating various pathologies; cell and tissue based therapies and other issues of low-temperature biology and medicine, as well as development of devices and equipment for low temperature biology and medicine.</p> <p>The Editorial Board takes the responsibility to provide a comprehensive peer-review of all submitted manuscripts. The Journal team strictly follows publishing ethics and standards to ensure high quality of scholarly publications. The Journal endorses the International Committee of Medical Journal Editors (ICMJE) <a href="http://www.icmje.org/recommendations/" target="_self">Recommendations for the Conduct, Reporting, Editing and Publication of Scholarly Work in Medical Journals</a>. (<a href="/journal/index.php/probl-cryobiol-cryomed/pubethics">Read more about Publishing Policy and Ethics</a>).</p> <p>Members of our Editorial Board are the specialists in various fields of low temperature biology and medicine from Ukraine and all over the world. (<a href="https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/about/editorialTeam">Editorial Board</a>).</p> <p>We do not charge the authors for article submission and/or processing and do not provide off-prints. The operation of the Journal is supported by National Academy of Sciences of Ukraine.</p> <p>Articles published in Problems of Cryobiology and Cryomedicine are Open-Access articles distributed under the terms and conditions of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/4.0/">CC BY</a>). We provide immediate open access to its content adhering to the principle that making research freely available to the public supports a greater global exchange of knowledge, increase visibility and readership. Use and distribution of the publications are permitted, retaining the credits to the authors and the Journal. The Journal holds the exclusive license to be the first publisher of the articles in print form and on-going non-exclusive license to disseminate the published content in various ways. The published version of the article could be deposited by authors to institutional or public repositories immediately on publication, stating a link to the URL of the published article on the journal's web-site.</p> <p>Our Journal is indexed/abstracted in <a href="http://www.scopus.com">Scopus</a>, <a href="http://thomsonreuters.com/en/products-services/scholarly-scientific-research/scholarly-search-and-discovery/zoological-record.html">Zoological Records</a>, <a href="http://www.cas.org/">Chemical Abstracts</a>, <a href="https://www.ebscohost.com/discovery?_ga=2.208532498.1058102411.1521647075-1463563202.1521647075">EBSCOhost</a>,&nbsp;<a href="http://bd.viniti.ru/">VINITI</a>, <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis64r_81/cgiirbis_64.exe?C21COM=F&amp;I21DBN=REF_EX&amp;P21DBN=REF&amp;S21CNR=20&amp;Z21ID=">Dzherelo </a>(Ukrainian Scientific Abstracts) and deposited in <a href="http://www.irbis-nbuv.gov.ua/cgi-bin/irbis_nbuv/cgiirbis_64.exe?Z21ID=&amp;I21DBN=UJRN&amp;P21DBN=UJRN&amp;S21STN=1&amp;S21REF=10&amp;S21FMT=juu_all&amp;C21COM=S&amp;S21CNR=20&amp;S21P01=0&amp;S21P02=0&amp;S21P03=PREF=&amp;S21COLORTERMS=0&amp;S21STR=KrioBiol">Vernadsky National Library of Ukraine Repository</a> (2002-now) and Google Books (<a href="https://books.google.co.jp/books?id=CSfuSZsuprkC&amp;dq=kriobiologiya&amp;hl=uk&amp;source=gbs_all_issues_r&amp;cad=1&amp;atm_aiy=1970#all_issues_anchor">1975-1990</a>; <a href="https://books.google.com.ua/books?id=B326AAAAIAAJ&amp;dq=problems+of+cryobiology&amp;hl=uk&amp;source=gbs_navlinks_s">1990-2002</a>).</p> <div id="gtx-anchor" style="position: absolute; visibility: hidden; left: 1473.54px; top: 296.181px; width: 9.88892px; height: 18.8889px;">&nbsp;</div> <div class="jfk-bubble gtx-bubble" style="visibility: visible; left: 575px; top: 325px; opacity: 1;" role="alertdialog" aria-describedby="bubble-2"> <div id="bubble-2" class="jfk-bubble-content-id"> <div id="gtx-host" style="min-width: 200px; max-width: 400px;">&nbsp;</div> </div> <div class="jfk-bubble-closebtn-id jfk-bubble-closebtn" tabindex="0" role="button" aria-label="Close">&nbsp;</div> <div class="jfk-bubble-arrow-id jfk-bubble-arrow jfk-bubble-arrowup" style="left: 1470.56px;"> <div class="jfk-bubble-arrowimplbefore">&nbsp;</div> <div class="jfk-bubble-arrowimplafter">&nbsp;</div> </div> </div> Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine en-US Problems of Cryobiology and Cryomedicine 2307-6143 <p>Authors who publish with this journal agree to the following terms:</p> <ul> <li class="show">Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a <a href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution License</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.</li> </ul> <ul> <li class="show">Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.</li> </ul> <ul> <li class="show">Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See <a href="http://opcit.eprints.org/oacitation-biblio.html" target="_new">The Effect of Open Access</a>).</li> </ul> Pilot Study of Hibernation Patterns in Noctule Bats (Nyctalus noctula): Influence of Group Size http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2001 <p>This study investigated the influence of group size on the hibernation patterns of adult males of Nyctalus noctula during hibernation. Bats were divided into three categories (solitary, groups of six, and groups of sixteen) and nine experimental groups comprising one individual with temperature logger per group (iButton). Skin temperature (Ts) was monitored every five minutes. The results showed that bats in larger groups (the group of sixteen) entered longer and more stable torpor, with no instances of full arousal (Ts &gt;20°C). In contrast, solitary bats exhibited more frequent arousals. All bats entered deep torpor after the first nine days. No significant differences in body mass loss were found between the groups. The findings highlight the importance of group size in reducing arousals during hibernation, and the role of social thermoregulation in conserving energy.</p> <p>&nbsp;</p> <p><em>Probl Cryobiol Cryomed 2024; 34(4):251–260</em></p> Anton Vlaschenko Marharyta Moiseienko Valeria Bohodist Anastasia Domanska Anatolii Goltvyanskiy Oleksandr Nahlov Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2025-04-04 2025-04-04 34 4 251–260 251–260 Optimization of Lyophilization Modes for Culture Medium Used in Mycobacterial Cultivation http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2002 <p>The paper presents the results of improving the lyophilization mode of egg-based culture medium for mycobacterial cultivation. The freezing of culture medium of 200 cm<sup>3</sup> volume at -30°C was established to provide more optimal conditions for lyophilization than other options we suggested here (200 cm<sup>3</sup> at –20°C and 300 cm<sup>3</sup> at –20 and –30°C). This enables to reduce the number of rejected vials and preserve the elective properties of the medium. Other freezing modes occurred to be less efficient. Our findings also showed the use of recommended modes for lyophilization as capable to accelerate the appearance of first signs of mycobacterial growth by 1–2 days.</p> <p><em>Probl Cryobiol Cryomed 2024; 34(4):261–267</em></p> <p>&nbsp;</p> Andriy Zavhorodniy Viktor Bilushko Svitlana Pozmohova Anatoliy Paliy Mykola Kalashnyk Olena Pavlichenko Karina Sviridova Oleksandra Tischenko Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2025-04-04 2025-04-04 34 4 261–267 261–267 Hsp70 as a Potential Biomarker of Tolerization of Cryopreserved Mononuclear Cells-Derived Dendritic Cells http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2003 <p>The work investigated the effect of three cryopreservation regimens of bone marrow mononuclear cells (MNCs) of CBA/H mice under the protection of a 10% dimethyl sulfoxide solution with a slow cooling rate of 1 deg / min: regimen 1 – to –80ºС; regimen 2 – to –40ºС; regimen 3 – to –25ºС with subsequent immersion of samples into liquid nitrogen (–196ºС) on the expression rate of heat shock protein with a molecular weight of 70 kDa (Hsp70) in MNCs and dendritic cells (DCs) obtained from them in vitro. The DCs tolerogenic potential was assessed after their use in adaptive therapy in mice with adjuvant arthritis by the ability to induce regulatory T cells (Treg) in spleen and minimize the arthritis index, as a clinical index of the pathology development. The dependence of expression rate of the Hsp70 protein in MNCs on the freezing mode was proven, as well as the signifi cance of this protein content in the manifestation of tolerogenic potential of DCs formed from them in vitro. The ability of cryopreservation according to regimen 2 to increase the Hsp70 content in MNCs was shown. The functional potential of DCs grown from cryopreserved MNCs with regimen 2 demonstrated their higher tolerogenic activity compared to DCs derived from native MNCs and cryopreserved with the regimens 1 and 3 MNCs. The maximum content of Hsp70 in DCs when using regimen 2 was consistent with that of Treg formed in animals with adjuvant arthritis after the administration of these cells, as well as it was accompanied by the most pronounced normalization of clinical signs of pathology. The findings demonstrate the ability of cryopreservation to regulate the Hsp70 expression rate as well as tolerogenic potential of DCs formed from cryopreserved precursors, increasing their use effectiveness in adaptive therapy of autoimmune diseases.</p> <p><em>Probl Cryobiol Cryomed 2024; 34(4):268–281</em></p> Anatoliy Goltsev Tetyana Dubrava Olena Lutsenko Yulia Gaevska Mykola Bondarovych Maksym Ostankov Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2025-04-04 2025-04-04 34 4 268–281 268–281 Therapy With Cryopreserved and Lyophilized Cord Blood Leukoconcentrates in Experimental Atopic Dermatitis http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2004 <p><strong><em>Probl Cryobiol Cryomed 2024; 34(4):282–286</em></strong></p> Hanna Koval Olena Lutsenko Mykola Bondarovych Igor Grisha Larysa Sokil Maksym Ostankov Anatoliy Goltsev Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2025-04-04 2025-04-04 34 4 282–286 282–286 48th Annual International Conference of Young Scientists "Cold in biology and medicine: current problems in cryobiology, transplantology, and biotechnology", Kharkiv, May, 15–16 2024 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2005 <p><em>Probl Cryobiol Cryomed 2024; 34(4):287–289</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 287–289 287–289 Reduction of the Toxicity of CPAs is Without Compromising the Cryoprotection in Sea Urchin Eggs (Part 1) http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2006 <p><em>Probl Cryobiol Cryomed 2024; 34(4):290</em></p> Uxía Rodríguez-Gavieiro Sara Campos Estefanía Pereira-Pinto Estefanía Paredes Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 290 290 Reduction of the Toxicity of CPAs is Without Compromising the Cryoprotection in Sea Urchin Eggs (Part 2) http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2007 <p><em>Probl Cryobiol Cryomed 2024; 34(4):290</em></p> Uxía Rodríguez-Gavieiro Sara Campos Estefanía Pereira-Pinto Estefanía Paredes Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 290 290 Stability of Mammalian Erythrocytes Under Varying Osmotic Conditions of the Medium http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2008 <p>Probl Cryobiol Cryomed 2024; 34(4):291</p> Olena Chabanenko Natalia Yershova Serhii Yershov Nataliia Shpakova Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 291 291 Low Temperatures Influence the Preservation of Regenerative Properties of Hyaluronic Acid with Different Molecular Weight http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2009 <p><em>Probl Cryobiol Cryomed 2024; 34(4):291</em></p> Anatolii Seliuta Eduard Nardid Anna Polyakova Tetiana Gurina Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 291 291 Cryopreservation of Shoot Tips of Valeriana jatamansi, a High Value Threatened Medicinal Plant of India http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2010 <p><em>Probl Cryobiol Cryomed 2024; 34(4):292</em></p> Ravi Gowthami Subhash Chander Anuradha Agrawal Neelam Sharma Kailash Chandra Bhat Anju Mahendru Singh Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 292 292 A Wide Spectrum Droplet Vitrification Cryopreservation Protocol for Conservation of Hops (Humulus lupulus) http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2011 <p><em>Probl Cryobiol Cryomed 2024; 34(4): 292</em></p> Era Malhotra Suresh Mali Shreya Sharma Sangita Bansal Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 292 292 Effect of Co-Culturing Neural Cells with MSCs on Spheroid Formation and Their Recovery After Cryopreservation http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2012 <p><em>Probl Cryobiol Cryomed 2024; 34(4):293</em></p> Svitlana Vsevolodska Olha Ochenashko Olha Maiorova Oleksandr Sukach Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 293 293 Short-Term Storage of Core-Shell Encapsulated MSCs at Ambient Temperature http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2013 <p><em>Probl Cryobiol Cryomed 2024; 34(4):293</em></p> Oleksandra Hubenia Sara Leal-Marin Natalia Trufanova Birgit Glasmacher Oleksandr Petrenko Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 293 293 The Experience of Using Cryotechnology in Animal Breeding to Ensure Food Security in Ukraine http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2014 <p>Probl Cryobiol Cryomed 2024; 34(4):294</p> Anastasiia Bogdaniuk Maryna Petrushko Volodymyr Garkavii Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 294 294 Different Approaches for Mesenchymal Stromal Cells Isolation from Wharton’s Jelly Tissue http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2015 <p>Probl Cryobiol Cryomed 2024; 34(4): 294</p> Ruslana Oberemok Natalia Trufanova Oleh Trufanov Olena Revenko Oleksandr Petrenko Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 294 294 The Use of Cryopreserved Medications from the Feto-Placental Complex as a Method for Treating Infertility Caused by Chronic Endometritis: a Case Report http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2016 <p>Probl Cryobiol Cryomed 2024; 34(4):295</p> Oleksandr Feskov Lina Konoplia Anna Feskova Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 295 295 Therapeutic Effect of Umbilical Cord Blood and Placenta in the Treatment of Chronic Endometritis in Patients with Repeated Implantation Failures http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2017 <p><em>Probl Cryobiol Cryomed 2024; 34(4):295</em></p> Oleksandr Feskov Yevhenia Zhylkova Lina Konoplia Olena Blazhko Iryna Bezpechna Anna Feskova Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 295 295 The First Experience of Reproductive Function Restoration in Patients with Asherman's Syndrome Using Autologous Mesenchymal Stem Cells http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2018 <p><em>Probl Cryobiol Cryomed 2024; 34(4):296</em></p> Mykola Kozub Maksym Kozub Kseniia Skybina Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 296 296 Long-Term Storage of Seeds from Plant Gene Pool Samples in the National Depository http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2019 <p><em>Probl Cryobiol Cryomed 2024; 34(4):296</em></p> Yuliia Chernobai Viktor Riabchun Nataliia Kuzmyshyna Tetyana Shyianova Roman Bohuslavskyi Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 296 296 Thermoplastic Analysis of State Diagrams of Cryoprotective Solutions http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2020 <p><em>Probl Cryobiol Cryomed 2024; 34(4):297</em></p> Stanislav Sevastianov Oleksandr Osetsky Tetiana Gurina Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 297 297 Optimization of Hypothermic Storage of Canine Erythrocytes: the Role of Acetylcysteine in Increasing Mechanical Stability http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2021 <p><em>Probl Cryobiol Cryomed 2024; 34(4):297</em></p> Karyna Hrebeniuk Olha Denysova Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 297 297 Study of the Interaction of Antimicrobial Peptides, in Particular Gramicidin S With Cell Membranes http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2022 <p><em>Probl Cryobiol Cryomed 2024; 34(4):298</em></p> Igor Perepelitsa Volodymyr Berest Galyna Bozhok Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 298 298 Seasonal Dynamics of Changes in the Freezing Point of Natural Milk as a Criterion for Determining the Possible Amount of Added Water http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2023 <p><em>Probl Cryobiol Cryomed 2024; 34(4):298</em></p> Nataliia Rusko Valeriia Ponomarova Igor Korkh Anatoliy Paliy Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 298 298 Preparation of Ram Sperm Using Minimal Application Gradient for In Vitro Fertilization http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2024 <p><em>Probl Cryobiol Cryomed 2024; 34(4):299</em></p> Oksana Lyzohub Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 299 299 Selection of Sugars for Cryoprotective Media on MCF-7 Breast Cancer Cell Cultures http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2025 <p><em>Probl Cryobiol Cryomed 2024; 34(4):299</em></p> Bohdan Kopiak Olena Perepelitsina Mykhailo Sydorenko Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 299 299 Cultivation and Cryopservation of Protozoan Intestinal Parasites http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2026 <p><em>Probl Cryobiol Cryomed 2024; 34(4):300</em></p> Igor Kyrychenko Mykhailo Biriukov Sergiy Pokhil Olena Tymchenko Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 300 300 Experimental Justification of Effectiveness of Cord Blood Serum-Derived Biological Product for Correction of Autoimmune Thyroiditis http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2027 <p><em>Probl Cryobiol Cryomed 2024; 34(4):300</em></p> Yuliia Kurylko Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 300 300 Cryoresistance of Antarctic lichen Umbilicaria umbilicarioides Assessed by a Constant Rate Cooling Combined with Chlorophyll Fluorescence Data http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2028 <p><em>Probl Cryobiol Cryomed 2024; 34(4):301</em></p> Jiří Sekerák Jr Josef Hájek Anton Puhovkin Miloš Barták Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 301 301 Cryopreservation of Allium tuberosum Rottler ex Sprengel http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2029 <p><em>Probl Cryobiol Cryomed 2024; 34(4):301</em></p> Subhash Chander Ravi Gowthami Ruchira Pandey Anuradha Agrawal Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 301 301 Morphological Indices of Spleen Under Short-Term Hypothermic Exposure http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2030 <p><em>Probl Cryobiol Cryomed 2024; 34(4):302</em></p> Ruslan Aminov Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 302 302 Impact of Cryosublimation on Cord Blood Serum Composition http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2031 <p><em>Probl Cryobiol Cryomed 2024; 34(4):302</em></p> Anastasiia Skoryk Mariia Shevchenko Dmytro Salnykov Olga Prokopiuk Olga Gorina Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 302 302 Cord Blood Serum Composition After Low-Temperature Storage at –40°C http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2032 <p><em>Probl Cryobiol Cryomed 2024; 34(4):303</em></p> Dmytro Salnykov Olga Gorina Olga Prokopiuk Tetyana Mykhalchuk Mariia Shevchenko Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 303 303 Transcriptomic Analysis of Genes Expressed After Carotid Endarterectomy: Searching for New Pathways for Cerebral Ischemic Tolerance http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2033 <p><em>Probl Cryobiol Cryomed 2024; 34(4):303</em></p> Marek Furman Vladimir Sihotsky Michal Virag Ivan Kopolovets Miroslava Nemethova Rastislav Mucha Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 303 303 Cryopreservation Challenges and Cryoprotectant Efficacy in Cyprinodon variegatus Embryos http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2034 <p><em>Probl Cryobiol Cryomed 2024; 34(4):304</em></p> Guillermo Manuel Santos Francisco Rocha Estefanía Paredes Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 304 304 Migration Response of Astrocytes to the Effect of Conditioned Medium From Rat Bone Marrow Mesenchymal Stem Cells http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2035 <p><em>Probl Cryobiol Cryomed 2024; 34(4):304</em></p> Eva Székiová Zuzana Michalová Juraj Blaško Ivo Vanický Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 304 304 Molecular Dynamics Study of Ternary Water-NaCl-DMSO Mixtures During Freezing and Thawing http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2036 <p><em>Probl Cryobiol Cryomed 2024; 34(4):305</em></p> Ivan Klbik Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 305 305 Tomato Seed Yield Depending on Pre-Sowing Treatment http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2037 <p><em>Probl Cryobiol Cryomed 2024; 34(4):305</em></p> Maryna Shapko Nadiia Shevchenko Galyna Kovalenko Oleksandr Kuts Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 305 305 Effect of Cold on Blood Circulation in People With Different Somatotypes and Blood Pressure Levels http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2038 <p><em>Probl Cryobiol Cryomed 2024; 34(4):306</em></p> Liudmyla Horban Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 306 306 Effects of AIMР1/p43 and EMAP II Proteins on Malignant Tumor Cells http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2039 <p><em>Probl Cryobiol Cryomed 2024; 34(4):306</em></p> Mariia Kolomiiets Volodymyr Zayets Leonid Zaika Olga Bolsunova Oleksandr Kornelyuk Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 306 306 Hormonal Status of Aged Rats with Diet-Induced Obesity After Umbilical Cord Blood Administration http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2040 <p><em>Probl Cryobiol Cryomed 2024; 34(4):307</em></p> Olena Bondarenko Vladyslav Babiychuk Lyudmyla Babiychuk Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 307 307 Optimization of Human Umbilical Cord Mesenchymal Stem Cell Cryopreservation Protocol http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2041 <p><em>Probl Cryobiol Cryomed 2024; 34(4):307</em></p> Ilona Palii Olesia Redko Sofiia Lechachenko Bohdana Anna Dovhaliuk Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 307 307 Response of Cardiovascular System to the Effect of Cold in Individuals With Different Levels of Blood Pressure http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2042 <p><em>Probl Cryobiol Cryomed 2024; 34(4):308</em></p> Petro Tabas Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 308 308 Application of Cold to Diagnose the Functional State of Vestibular Analyzer http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2043 <p><em>Probl Cryobiol Cryomed 2024; 34(4):308</em></p> Roman Shmata Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 308 308 Thermosensitivity of Young People in Conditions of Extreme Climate Changes http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2044 <p><em>Probl Cryobiol Cryomed 2024; 34(4):309</em></p> Viktoriia Huk Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 309 309 Effect of Low Temperature Storage on Sensitivity to Antibiotics of Polyresistant Escherichia coli Strains http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2045 <p><em>Probl Cryobiol Cryomed 2024; 34(4):309</em></p> Olena Peretyatko Tetiana Gurina Yulya Yagnuk Svitlana Krestetska Nadia Sklyar Аndrii Yagnuk Oleksandr Pakhomov Halyna Bolshakova Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 309 309 Viability of Escherichia coli Strains After Long Term Storage in Cryoprotective Media http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2046 <p><em>Probl Cryobiol Cryomed 2024; 34(4):310</em></p> Yulya Yagnuk Tetiana Gurina Svitlana Krestetska Olena Peretyatko Nadia Sklyar Аndrii Yagnuk Oleksandr Pakhomov Halyna Bolshakova Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 310 310 Influence of Nucleated Cells of Human Cord Blood on Indices of Immune System of Animals with Atopic Dermatitis http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2047 <p><em>Probl Cryobiol Cryomed 2024; 34(4):310</em></p> Anatoliy Goltsev Hanna Koval Mykola Bondarovych Olena Lutsenko Maksym Ostankov Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 310 310 Cover of the Issue http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1997 Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2025-04-04 2025-04-04 34 4 Editorial Information http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1999 Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2025-04-04 2025-04-04 34 4 Content http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2000 <p><em>Probl Cryobiol Cryomed 2024; 34(4):250</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2025-04-04 2025-04-04 34 4 249 250 Backmatter http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2048 Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 Key Word Index Volume. Vol. 34 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2049 <p><em>Probl</em> <em>Cryobiol</em> <em>Cryomed</em><em> 2024; 34(4):314–316</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 311 316 Table of Contents Vol. 34 http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2050 <p><em>Probl</em> <em>Cryobiol</em> <em>Cryomed</em><em> 2024; 34(4):321–324</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 317 324 Instruction for Authors http://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/2051 <p><em>Probl Cryobiol Cryomed 2024; 34(4):327-328</em></p> Editorial Board Problems of Cryobiology and Cryomedicine Copyright (c) 2025 https://creativecommons.org/licenses/by/4.0 2024-12-26 2024-12-26 34 4 325 328