Problems of Cryobiology and Cryomedicine

Modern Approaches and Perspectives of Human Cord Blood Nucleated Cells’ Freeze-Drying

Sun, 10 Mar 2024 00:00:00 +0100

The review summarizes data on the prospects of using lyophilization as a methodical approach to preserve structure and functions for a long-term storage of human cord blood leukoconcentrate (HCBL). The advantages of the freeze-drying compared to low-temperature storage, natural or thermal drying have been shown. The question of the impact of physical and chemical factors, which are implemented at the stage of lyophilization, on structure and functions of HCBL cells has been considered. The importance of such factors as cell concentration, storage conditions, residual moisture in ensuring the preservation of lyophilized material was disputed. The paper analyzes the data on the use of lyoprotectants and antioxidants during freeze-drying as well as discusses the safety of using lyoprotectant delivery methods in relation to the genomic profile of cells.

Probl Cryobiol Cryomed 2023; 33(4): 227–249

Study of Release of Biologically Active Compounds from Cord Blood Under Different Conditions of Low-Temperature Impact

Sat, 30 Dec 2023 00:00:00 +0100

Here, we have studied the impact of cord blood destruction method on composition of the cord blood-derived low molecular fractions, and compared the cryodestruction with other methods of cell destruction before extracting. Human cord blood was destroyed by rapid or slow freezing / warming, hypotonic lysis and thermal destruction. The obtained substance was used to produce the cord blood fraction (CBF) by multi-stage ultrafiltration and lyophilization. Dry weight, CBF composition and total protein content in them were evaluated by chromatographic profiles (gel permeation and reverse-phase high-performance liquid chromatography). The CBFs, obtained by different techniques for cord blood destruction were established to differ in the content and molecular weights of the components. These findings suggest the possibility to vary the amount and range of low molecular weight compounds in lyophilized cord blood fractions by using low temperatures and combining different regimens of freezing / warming.

Probl Cryobiol Cryomed 2023; 33(4): 250–262

Low Temperature Influence on Activity in Alginate Microcapsules Incorporated-Enzymes

Iryna P. Khala, Kateryna D. Rozanova, Svitlana V. Repina, Oleg A. Nardid — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4): 263–267

Changes in Blood Leukocyte Composition in Rats of Different Ages after Long-term Cold Adaptation

Viktoria V. Lomako — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4): 268–273

Cryopreserved Placental Derivatives Improve Quality of Life and Behavioral Responses in Mice with Chemotherapy-Induced Ovarian Failure

Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4): 274–279

90th birthday of Prof. Tetiana M. Yurchenko

Editorial Board Problems of Cryobiology and Cryomedicine — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):296-297

Abstracts of the 47th Annual Conference of Young Scientists ‘Cold in Biology and Medicine. Current Issues in Cryobiology, Transplantology and Biotechnology’ May 23, 2023, Kharkiv, Ukraine

Editorial Board Problems of Cryobiology and Cryomedicine — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):280-282

The Effect of Varying Thawing Temperatures and Preserving Conditions on the Recovery Rate of Cryopreserved Equine Dendritic Cells

Tarek Deeb, Felix Schwebel, Tim Rittinghaus, Birgit Glasmacher — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):283

Hatching of Cryopreserved Human Blastocysts Obtained in IVF and ICSI Cycles

Nataliia Shapovalova, Taisiia Yurchuk, Volodymyr Pinyaev, Maryna Petrushko — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):283

Correlation Between Biochemical Parameters of Seminal Plasma and Cryoresistance of Spermatozoa of Saanen Bucks

Anastasiia Bogdaniuk, Maryna Petrushko, Volodymyr Garkavii — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):284

Potential Ways for Fish Biodiversity Conservation Within Siversky Donets Basin in Ukraine Using Cryobiology and Cryobanking Techniques

Daniil Ostras, Hennadiy Honcharov, Kostiantyn Mikson — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):284

Preservation of Human Mesenchymal Stromal Cells in Protein-Supplemented Alginate Capsules

Oleksandra Hubenia, Sara Leal Marin, Natalia Trufanova — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):285

Сryoresistance of Autotrophic Organisms From Polar Regions Sensed by Chlorophyll Fluorescence Method

Davide Giordano, Anton Puhovkin, Josef Hájek, Jiří Sekerák, Miloš Bartak — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):285

Development of a Numerical Simulation Model for Continuous Floating Cryopreservation of Red Blood Cell Concentrate

Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):286

Impact of Environmental Factors on the Moss Banks’ State in Argentine Islands (West Antarctica): Interannual Monitoring

Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):286

Discriminating Cells by Membrane Surface Charge May Improve Long-Term Refrigerated Storage of Red Blood Cells

Daniil Liadov, Volodymyr Berest — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):287

Software Solution for Moving Objects Identification and Tracking Individual Behavior in Studies of Fish Adaptation for Sub-Ambient Temperatures

Polina Berest — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):287

Features of Formation and Cryopreservation of Spheroids From Brain Cells of Newborn Rats

Svitlana Vsevolodska, Oleksandr Sukach, Olga Ochenashko — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):288

Effect of Low Temperatures on Morphology of Alginate Microcarriers

Stanislav Narozhnyi, Olena Bobrova, Igor Kovalenko — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):288

Comparative Analysis of Sensitivity of Canine and Human Erythrocytes to Changes in Osmotic Conditions of the Medium

Yevhenii Hubenia, Serhii Yershov, Olena Chabanenko, Nataliia Shpakova — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):289

The Effect of Salinity on the Resistance of Plant Cells to Low and Ultra-Low Temperatures on the Example of Dunaliella salina Microalgae Cells

Nadiia Chernobai, Krystyna Vozovyk — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):289

Cytotoxicity and Cryoprotective Efficiency of Polyvinyl Alcohol Solutions During Freezing of Human Erythrocytes

Yana Hvozdiuk, Yulia Pakhomova, Valentina Chekanova, Antonina Kompaniets — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):290

Optimization of Methods for Obtaining and Evaluating Organotypic Liver Cultures for Further Cryopreservation

Аnna Kaverinska, Volodymyr Prokopiuk — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):290

Peculiarities of the Structure of Frozen Cryoprotective Solutions

Stanislav Sevastianov, Oleksandr Osetsky — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):291

Paclitaxel Induces Axonal Degeneration and Alters Cold Temperature Sensitivity in Adult Rats

Zuzana Michalová, Ivo Vanický — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):291

Morphofunctional Feature of the Formed Elements of Blood Cells and the Central Lymphoid Organ Under Conditions of Short-Term Hypothermic Exposure

Ruslan Aminov — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):292

Characterization of the Hepatoprotective Activity of Placenta Cryoextract in Liver Lesions of Various Etiologies

Illia Koshurba, Mykola Chyzh, Fedir Hladkykh — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):292

Fipronil Impairs Proliferation in the Olfactory Neurogenic Region and Alters Low Temperature Sensitivity in Adult Mice

Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):293

Effect of Medium Conditioned by Cryopreserved Placental Cells on Organotypic Ovarian Culture

Tetyana Mykhalchuk, Volodymyr Prokopiuk, Mariia Shevchenko, Olga Prokopiuk — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):293

Electrospun Scaffolds for the Hypothermic Preservation of Cells

Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):294

Long-Term Results of Cryosurgery and Combined Treatment of Patients With Deep Brain Tumors

Vladyslav Kaliuzhka, Volodymyr Piatykop, Oleksandra Cherniaieva — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):294

Prospects for the Use of Cryopreserved Bone Marrow Mesenchymal Stem Cells in the Treatment of Neurological Diseases and Injuries

Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):295

Development of Electrodes for Cryopreservation of Stem Cells by Electroporation-Assisted Loading with Sucrose

Anna Schwäblein, Diaa Khayyat, Sara Leal Marin, Birgit Glasmacher — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):295

Cover of the Issue

Editorial Board Problems of Cryobiology and Cryomedicine — Sun, 10 Mar 2024 00:00:00 +0100

Editorial Information

Editorial Board Problems of Cryobiology and Cryomedicine — Sun, 10 Mar 2024 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):226

Key Word Index Vol. 33

Editorial Board Problems of Cryobiology and Cryomedicine — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 32(4):301–303

Table of Contents Vol. 33

Editorial Board Problems of Cryobiology and Cryomedicine — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):308–311

Instruction for Authors

Editorial Board Problems of Cryobiology and Cryomedicine — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):314-315

Backmatter

Editorial Board Problems of Cryobiology and Cryomedicine — Sat, 30 Dec 2023 00:00:00 +0100

Probl Cryobiol Cryomed 2023; 33(4):316