Effect of Cryopreservation Conditions on Phenotypic Profile and Immunomodulating Activity of Fetal Liver Cells

Authors

  • Maksim V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Larisa A. Leonova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Nikolay A. Bondarovich Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Lyudmila V. Ostankova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Anatolyi M. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo29.03.266

Keywords:

cryopreservation, DMSO, rat fetal liver cells, immunomodulating activity, experimental atopic dermatitis

Abstract

The effect of cryopreservation conditions (concentration of dimethyl sulfoxide cryoprotector − DMSO, cooling rate in different temperature ranges) on phenotypic profile and immunomodulatory activity of rat fetal liver cells (FLC) of 14 days of gestation was investigated. The cells were frozen in two modes under the protection of 7.5; 10, and 12.5% DMSO. We evaluated the phenotypic profile of FLCs (CD34+CD38–, Sca-1+CD34-, Sca-1+CD34+, CD44-CD73+, CD44+CD73+), their cytomorphological indices, number and viability. The FLCs cryopreservation mode developed in this work ensured the preservation in a heterogeneous cell population with the immunomodulating activity, which was evaluated in the in vivo system on the model of experimental atopic dermatitis (EAD). The efficacy of using cryopreserved FLCs in mode 1 (1°С / min to –25°С; stabilization at –25°С for 5 min and plunging in liquid nitrogen) with 10% DMSO to restore blood parameters and reduce the intensity of skin inflammatory reactions in rats with induced EAD was experimentally established.

 

Probl Cryobiol Cryomed 2019; 29(3): 266–276.

Author Biographies

Maksim V. Ostankov, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Larisa A. Leonova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Nikolay A. Bondarovich, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Lyudmila V. Ostankova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

Anatolyi M. Goltsev, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryopathophysiology and Immunology

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Published

2019-09-19

How to Cite

Ostankov, M. V., Leonova, L. A., Bondarovich, N. A., Ostankova, L. V., & Goltsev, A. M. (2019). Effect of Cryopreservation Conditions on Phenotypic Profile and Immunomodulating Activity of Fetal Liver Cells. Problems of Cryobiology and Cryomedicine, 29(3), 266–276. https://doi.org/10.15407/cryo29.03.266

Issue

Section

Cryopreservation of Biological Resources