Kinetic Characteristics of Ca2+-ATPase of Erythrocytes in PEO-1500 Presence

Authors

  • N. G. Zemlyanskikh
  • L. A. Babijchuk
  • A. Yu. Nikolchenko
  • V. P. Tyagileva

Keywords:

erythrocytes, Ca2 -ATPase, cryoprotectants, polyethylene oxide

Abstract

In the work it has been established, that the exocellular cryoprotectant PEO-1500 inhibits the activity of Ca2+-ATPase of erythrocytes. Kinetic analysis showed that the inhibition of enzyme activity was related to the Vmax alteration, but not to Km for catalytic center with a low affinity of Ca2+-ATPase. The highly affinic center of Ca2+-ATPase was not modified under PEO-1500 effect. The presence of cryoprotectant in the medium reduces the sensitivity of enzyme response of ATP hydrolysis to Ca2+ ions, as the result the erythrocyte Ca2+-ATPase functioning is activated in a lesser extent within the range of Ca2+ low concentrations and is inhibited in a greater extent for a high concentration range of this ion in comparison with the native erythrocytes. These data allow to suggest, that under PEO-1500 effect the Ca2+-ATPase ion-transporting function can be suppressed, and the efficiency of Ca-pump activity will decrease. The inhibition of Ca2+-ATPase activity is evidently caused by modifying the physical and chemical characteristics of plasma membrane under PEO-1500 effect.

Author Biographies

N. G. Zemlyanskikh

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

L. A. Babijchuk

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

A. Yu. Nikolchenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

V. P. Tyagileva

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Downloads

Published

2003-12-25

How to Cite

Zemlyanskikh, N. G., Babijchuk, L. A., Nikolchenko, A. Y., & Tyagileva, V. P. (2003). Kinetic Characteristics of Ca2+-ATPase of Erythrocytes in PEO-1500 Presence. Problems of Cryobiology and Cryomedicine, (4), 28–34. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1075

Issue

No. 4 (2003): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).