Sorption of Bromthymol Blue with Methaemoglobin of Donor and Placental Blood in Ethylene Glycol and PEG-3000 Solutions

Authors

  • Yu. V. Kucherenko
  • B. N. Leonov
  • K. D. Rozanova

Keywords:

methaemoglobin, ethylene glycol, PEG-3000, dye sorption

Abstract

The authors studied the sorption of bromothymol blue (BTB) by donor and placental blood haemoglobin in ethylene glycol (EG) and PEG-3000 solutions with the same value of dielectric constant, εs. The performed investigations show that there is no distinct correlation between εs and parameters of BTB sorption by methaemoglobin from donor and placental blood. In addition, the obtained data testify to the possible sorption of PEG-3000 molecules on protein surface.

Author Biographies

Yu. V. Kucherenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

B. N. Leonov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

K. D. Rozanova

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

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Published

2003-12-25

How to Cite

Kucherenko, Y. V., Leonov, B. N., & Rozanova, K. D. (2003). Sorption of Bromthymol Blue with Methaemoglobin of Donor and Placental Blood in Ethylene Glycol and PEG-3000 Solutions. Problems of Cryobiology and Cryomedicine, (4), 66–69. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/1080

Issue

No. 4 (2003): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

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