Electric Conductivity and Resistance of Mouse Oocyte Membranes to Effect of Pulsed Electric Field in Cryoprotectant Solutions
DOI:
https://doi.org/10.15407/cryo28.04.311Keywords:
mouse oocytes, plasma membrane, electric conductivity, cryoprotectant, electroporation, electric breakdown, pulsed electric ï¬eld, intensityAbstract
Using the method of pulsed conductometry, the values of electric conductivity of mouse oocytes in 1.0 M solutions of penetrating cryoprotectants have been first determined and the stability of their plasma membranes to the effect of pulsed electric field of increasing intensity has been investigated. For the oocytes, incubated in ethylene glycol (EG), 1,2-propanediol (1,2-PD), 2,3-butanediol (2,3-BD) and dimethyl sulfoxide (DMSO) solutions, the electric conductivity values were (23.6 ± 3.4), (21.3 ± 5.8) and (21.0 ± 2.3) and (23.6 ± 7.1) μS/cm respectively. The values of electric conductivity for mouse oocytes in acetamide (AC) and formamide (FA) solutions were (16.5 ± 6.1) and (16.9 ±± 10.7) μS/cm, respectively. Cryoprotectant solutions, belonging to alcohols (EG, 2,3-BD), as well as DMSO, were shown to have a stabilizing effect on the mouse oocyte plasma membranes under electric field action. The AC and FA solutions caused no stabilizing effect on mouse oocyte membranes, which was manifested in development of irreversible electric breakdown of plasma membranes with increasing electric field intensity.
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Probl Cryobiol Cryomed 2018; 28(4): 311-321
References
Best BP. Cryoprotectant toxicity: facts, issues, and questions. Rejuvenation Res 2015; 18(5): 422–36.
Chekurova NR, Kislov AN, Veprintsev BN. [The effect of cryoprotectants on electric parameters of mouse embryo cell membranes]. Kriobiologia. 1990; (1): 25–9. Russian. Full Text
Chernomordik LV. Electropores in lipid bilayers and cell membranes. in: Chang. DC, Chassy BM, Saunders JA, Sowers AE, editors. Guide to electroporation and electrofusion. London: Academic Press. 1992. p 63–76.
Crowe JH, Hoekstra FA, Crowe LM, et al. Lipid phase-transitions measured in intact-cells with Fourier-transform infrared-spectroscopy. Cryobiology. 1989; 26(1): 76–84.
Damaskin BB, Petriy JF, Tsirlina GA. [Electrochemistry]. Moscow; Khimia KoloS, 2006. 672 p. Russian
Elliott GD., Wang S., Fuller BJ. Cryoprotectants: a review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures. Cryobiology. 2017 June; 76: 74–91. CrossRef PubMed
Fahy GM. The relevance of cryoprotectant 'toxicity' to cryo- biology. Cryobiology. 1986; 23(1):1–13.
Fernández ML, Reigada R. Effect of dymethyl sulfoxide on lipid membrane electroporaton. J Phys Chem. 2014; 118(31): 9306–12.
Karapyetyan YuA, Eichys VN. [Physico-chemical properties of electrolyte non-aqueous solutions]. Moscow; Khimia; 1989. 256 p. Russian.
Larman MG, Minasi MG, Rienzi L, Gardner DK. Maintenance of the meiotic spindle during vitriï¬cation in human and mouse oocytes. Reprod Biomed Online [Internet]. 2007 Dec [Cited 13.09.2018]; 15(6): 692–700. Available from: www.rbmonline.com/Article/2987 PubMed
Linnik TP, Bizikina OV. Fowl sperm cryopreservation. I. Cytotoxicity of diols and amides. Problems of Cryobology. 2001; (2):72–9. Full Text
Mandawala AA, Harvey SC, Roy TK, Fowler KE. Cryopreservation of animal oocytes and embryos: Current progress and future prospects. Theriogenology. 2016; 1586(7): 1637–44.
Mank M, editor. [Developmental biology of mammals. Methods]. Moscow; 1990. 406 p. Russian.
Moussa M, Shu J, Zhang X, Zeng F. Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives. Sci China Life Sci. 2014; 57(9): 903–14.
Pichugin YuI. Results and perspectives in searching of new endocellular cryoprotectants. Problems of Cryobiology 1993; (2): 3–10. Full Text
Pogorelov AG, Katkov II, Smolyaninova EI, Goldshtein DV. Changes in intracellular potassium and sodium content of 2-cell mouse embryos induced by exposition to vitriï¬cation concentrations of ethylene glycol. Cryo-Letters. 2006; 27(2): 87–98. PubMed
Rols MP. Electropermeabilization, a physical method for the delivery of therapeutic molecules into cells. Biochim Biophys Acta 2006; 1758 (3): 423–8.
Shevchenko NA, Strybul TF, Rozanov LF. Effect of multiatom alcohols, amides and DMSO on grape and potato meristems integrity. Problems of Cryobiology. 2004; (3): 79–85. Full Text
Shigimaga VA. Pulsed conductometer for biological cells and liquid media. Measurement Techniques. 2013; 55(11): 1294–300.
Smolaninova EI, Shigimaga VF, Kolesnikova AA, et al. [The stage of murine oocyte maturation correlates with their electric conductivity.] The Animal Biology 2015; 17(1): 118–25. Ukrainian.
Smolyaninova EI, Shigimaga VA, Strikha OA et al. [Electric conductivity as a diagnostic parameter for mammalian oocyte and embryo quality estimation in biotechnology operations.] Biophisika zhivoi kletki 2014; (10): 193–5. Russian.
Smolyaninova EI, Shigimaga VA, Strikha OA, et al. Effect of cryopreservation stages by vitriï¬cation in ethylene glycoland sucrose medium on 2-cell murine embryos electric conductivity. Probl Cryobiol Cryomed. 2013; 23(3): 228–39. Full Text
Smolyaninova EI, Strikha OA, Shigimaga VA, et al. Electric conductivity of murine embryos at preimplantation developmental stages after ovary hormonal stimulation in animal. Biotechnologia Acta. 2013; 6(1): 105-12.
Smolyaninova EI, Shigimaga VA, Kolesnikova AA. [The effect of Hormone stimulation on morphological and electric parameters of murine oocytes.] The Animal Biology. 2009; 11(1-2): 329–38. Ukrainian.
Szusek EF, Eroglu A. Comparison and avidance of toxity of penetrating cryoprotectants. PLoS One[Internet]. 2011 Nov 16 [Cited13.09.2018];6(11):e27604.Availablefrom:https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0027604. CrossRef PubMed
Tsong TY. Electroporation of cell membrane. Biophys J. 1991;60(2): 297–306.
Weaver JC. Theory of electroporation: a review. Bioelectrocem Bioenerg. 1996; 41(2): 135–160.
Zimmermann U. Electric ï¬eld mediated fusion and related electrical phenomena. Biochim Biophis Acta. 1982; 694(3): 227–77.
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