Influence of Freeze-Thawing with DMSO on Integrity and Osmotic Fragility of Erythrocyte from Different Mammalian Species

Authors

  • P. Yu. Ulizko Kharkov State Zoo-Veterinary Academy, Kharkov region
  • G. F. Zhegunov Kharkov State Zoo-Veterinary Academy, Kharkov region
  • O. N. Denisova Kharkov State Zoo-Veterinary Academy, Kharkov region

Keywords:

mammal erythrocytes, hemolysis, dimethyl sulfoxide, cryopreservation, osmotic fragility

Abstract

In the research there was studied the hemolysis level of bovine, equine, and rabbit erythrocytes in different hypotonic media after incubation and freeze-thawing with 10% dimethyl sulfoxide (DMSO) solution. It was shown, that intact erythrocytes of all the examined animals demonstrated the similar osmotic stability in hypotonic conditions, and DMSO in the stated concentration does not significantly affect this parameter and red blood cell integrity. The use of 10% DMSO during freeze-thawing without washing-out the cryoprotectant allows to preserve up to 80% erythrocytes, however, the process of cryoprotectant removal reduces this index.

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Published

2011-02-21

How to Cite

Ulizko, P. Y., Zhegunov, G. F., & Denisova, O. N. (2011). Influence of Freeze-Thawing with DMSO on Integrity and Osmotic Fragility of Erythrocyte from Different Mammalian Species. Problems of Cryobiology and Cryomedicine, 21(1), 52–57. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/154

Issue

Vol. 21 No. 1 (2011): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 P. Yu. Ulizko, G. F. Zhegunov, O. N. Denisova

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