Study of Possibility to Obtain and Cryopreserve Adrenal Cell Primary Culture of Newborn Piglets

Authors

  • O. S. Sidorenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • G. A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. I. Legach Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

adrenal fragments, primary culture of adrenal cells, cryopreservation, viability, adhesion

Abstract

There was investigated the effect of method of biological material cryopreservation (composition of cryoprotective medium, cooling rate, cryopreservation of tissue fragments or primary cell culture) on viability of newborn piglets adrenal cells as well as their behavior during further culturing. The cryopreservation method, enabling to achieve a high survival and viability of cells as well as to preserve their ability to adhesion and flattening during further culturing was developed.

Downloads

Published

2011-02-21

How to Cite

Sidorenko, O. S., Bozhok, G. A., Legach, E. I., & Gurina, T. M. (2011). Study of Possibility to Obtain and Cryopreserve Adrenal Cell Primary Culture of Newborn Piglets. Problems of Cryobiology and Cryomedicine, 21(1), 58–67. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/155

Issue

Vol. 21 No. 1 (2011): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

License

Copyright (c) 2020 O. S. Sidorenko, G. A. Bozhok, E. I. Legach, T. M. Gurina

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).