Cryopreservation of Multicellular Spheroids Derived from Primary Culture of Newborn Piglet Spinal Ganglion Cells
DOI:
https://doi.org/10.15407/cryo29.04.354Keywords:
dorsal root ganglia, multicellular spheroids, satellite glial cells, ï¬broblast-like cells, neuron-like cells, neonatal pigletsAbstract
Dorsal root ganglia (DRG) are a potential source of neural stem cells, since they contain the neural crest-derived cells capable to differentiate into neurons and different subpopulations of glial cells. It is known that under certain culture conditions, the DRG cells, derived from neonatal piglets form the floating multicellular spheroids (MSs), capable to produce the ï¬broblast-like cells (FLCs), glial cells (GCs) and neuron-like cells (NLCs). Here, we have cryopreserved the MSc, derived under serum-free culture conditions using 5, 7.5, 10% dimethyl sulfoxide (DMSO) and two-stage regimen of freezing. After cryopreservation, the MSs were established to preserve the capability to adhere as well as to produce three cell types: FLCs, GCs and NLCs. However, the capability of frozen-thawed MSs to produce different cell types was reduced. The cryoprotectant concentration affected the cell type, prevailing in the culture, derived from the frozen-thawed MSs. After cryopresevation with 10% DMSO, the monolayer consisted mainly of FLCs, while with 5 and 7.5% DMSO it mostly comprised GLCs.
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Probl Cryobiol Cryomed 2019; 29(4):Â 354-364
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