Comparative Evaluation of Biological Activity of Fraction Below 5 kDa from Cattle Cord Blood After Low-Temperature Storage (at -80°C) or Lyophilization to Treat Burn Wounds in Rats
DOI:
https://doi.org/10.15407/cryo30.01.047Keywords:
low molecular weight fraction, cord blood, low temperature storage, lyophilization, biological activity, burnAbstract
Biological activity of the low molecular weight (down to 5 kDa) fraction from cattle cord blood after storage in lyophilized state at 4°C and -80°C in the form of solution in a model of thermal burn of rats' skin was investigated. Actovegin with a pronounced wound healing activity was used as a comparator drug. The storage of low molecular weight fraction for 3 months under the chosen conditions was found not to reduce its biological activity. Introduced and lyophilized cord blood fraction to experimental animals after simulation of thermal skin burns contributed to a reduced burn wound area, normalization of leukocyte content and phagocytic activity of neutrophils in peripheral blood. The findings enable considering the lyophilization of the low molecular weight fraction followed by storage at 4°C as sensibly more expedient option of storing and transporting the biologically active substance.
Probl Cryobiol Cryomed 2020; 30(1): 47-57
References
Agita A, Alsagaff MT. Inflammation, immunity, and hypertension. Acta Med Indones. 2017 ; 49(2): 158-65.
Belous AM, Tsvetkov DU. [Scientific grounds of the technology of sublimational preservation]. Kyiv: Naukova Dumka; 1985. 208 p. Russian.
Brock TD. Membrane filtration: a user's guide and reference manual. Berlin-New York: Springer-Verlag; 1983. 381 p.
Damien P, Allan DS. Regenerative therapy and immune modulation using umbilical cord blood-derived cells. Biol Blood Marrow Transplant. 2015; 21(9): 1545-54. CrossRef
Emami F, Vatanara A, Park EJ, et al. Drying technologies for the stability and bioavailability of biopharmaceuticals. Pharmaceutics [Internet]. 2018 [cited 2018 Aug 18]; 10(3): E131. Available from: https://www.mdpi.com/1999-4923/10/3/ 131/htm CrossRef
Fuller B, Green C, Grischenko VI. Cryopreservation for Ñell banking: current concepts at the turn of the 21st century. Problems of Cryobiology. 2003; 13(2): 62-83.
Gulevsky AK, Moiseyeva NN, Abakumova YS, et al. [Studying of biological activity of fraction less than 5 kD from cattle cord blood cryohaemolizate]. Problems of Cryobiology. 2008; 18(4): 531-4. Russian.
Gulevsky AK, Moiseyeva NN, Gorina OL. Influence of low-molecular (below 5 kDa) fraction from cord blood and actovegin on phagocytic activity of frozen-thawed neutrophils. CryoLetters. 2011; 32(2): 131-40. CrossRef
Gulevsky AK, Moisieieva NN, Gorina OL, et al. [The influence of low-molecular fraction from cord blood (below 5 kDa) on functional and biochemical parameters of cells in vitro]. Ukr Biochem J. 2014; 86(6): 167-74. Russian. CrossRef
Gulevsky AK, Nikolchenko AYu, Somov AYu, et al. [Influence of the fraction below 5 kDa from cattle cord blood on respiratory activity of rat liver homogenates]. Vestnik problem biologii i mediciny. 2012; 9(1): 104-6. Russian.
Gulevsky AK, Shenyavsky II, Nikolchenko AYu. [Cord blood and its components: biological features, clinical applying, storage in cryobanks]. Kharkiv: Rider; 2017. 344 p. Russian
Jang TH, Park SC, Yang JH, et al. Cryopreservation and its clinical applications. Integr Med Res. 2017; 6(1): 12-8. CrossRef
Kozinets GI, Makarova VA. [Study of the blood system in clinical practice]. Moscow: Triada; 1998. 480 p. Russian.
Mayadas TN, Cullere X, Lowell CA. The multifaceted functions of neutrophils. Annu Rev Pathol. 2014; 9: 181-218. CrossRef
Moiseyeva NN. [The influence of a low molecular fraction (less than 5 kD) from cord blood on regeneration processes in burn wounds in rats]. World of Medicine and Biology. 2009; (3): 117-20. Russian.
Obermaier-Kusser B, Mühlbacher C, Mushack J, et al. Further evidence for a two-step model of glucose-transport regulation. Inositol phosphate-oligosaccharides regulate glucose-carrier activity. Biochem J. 1989; 261(3): 699-705. CrossRef
Osetskiy AI, Grischenko VI, Snurnikov AS, et al. Cryosublimation fractionating of biological material. Problems of Cryobiology. 2006; 16 (2): 230-40.
Rozanova OE, Serova LD, Shabalin VN. [The influence of cytotoxic and hormonal drugs on the phagocytic activity of neutrophils in patients with leukemia]. Gematologiya i transfuziologiya. 1989; 34(4): 15-20. Russian.
Rumyantseva SÐ. [Actovegin. New aspects of clinical application]. Moscow; 2002. 280 p. Russian.
Svedentsov EP, Tumanova TV, Khudyakov AN, et al. [Cryo-preservation of functionally active blood nuclear cell membranes at −80°C]. Biochemistry (Moscow) Supplement: Membrane and Cell Biology. 2008; 2(1): 19-25. Russian.
Yelsky VN, Strelchenko YuI, Zyablitzev SV. [Changes in phagocytic immunity in rats with dosed burn injury and subsequent exposure to polarized light]. Travma. 2012; 13(4): 70-4. Ukrainian.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Oleksandr Gulevskyy, Natalia Moiseyeva, Olga Gorina, Andriy Nikolchenko, Ivan Schenyavsky
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
