Effect of Cooling Rate on Integrity of Zone-Differentiated Adrenal Cell Populations of Rats

Authors

  • G. V. Dudetskaya Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • G. A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

fluorescent dyes, 3beta-hydroxysteroid dehydrogenase, chromogranin A, cryopreservation, cooling rate, adrenal cell suspension

Abstract

By means of the fluorescent microscopy and histochemical staining the adult rat adrenal cells prior to and after cryopreservation were studied. Low cooling rates enable the preservation of the highest number of cells in suspension after freeze-thawing. Cooling rates cause a selective effect on preservation of the cells of cortical and medullar substances of adrenal glands.

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Published

2011-01-10

How to Cite

Dudetskaya, G. V., Bozhok, G. A., & Bondarenko, T. P. (2011). Effect of Cooling Rate on Integrity of Zone-Differentiated Adrenal Cell Populations of Rats. Problems of Cryobiology and Cryomedicine, 20(4), 379–387. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/168

Issue

Vol. 20 No. 4 (2010): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 G. V. Dudetskaya, G. A. Bozhok, T. P. Bondarenko

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