Effect of Amphiphiles on Hypertonic Hemolysis of Erythrocytes, Modified with Temperature of 49°C

Authors

  • N. A. Pisarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. M. Shpakova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

hypertonic stress, erythrocytes, spectrin, amphiphilic compounds

Abstract

The effect of thermal pre-treatment of human and bovine erythrocytes on their sensitivity to hypertonic stress (4.0 mol/l NaCl) at 37 and0°Cwas studied. It has been shown that cell incubation at49°C, causing spectrin denaturation, decreases the level of human erythrocyte hypertonic damage and does not affect the integrity of bovine erythrocytes at37°C. Hypertonic hemolysis of modified mammalian erythrocytes does not change at0°C. Cytoskeletal spectrin denaturation of bovine and human erythrocytes results in reduction of antihemolytic activity of amphiphilic substances (sodium decyl sulfate, dodecyl-β,D-maltoside, trifluoroperazine) at 37 and0°C.

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Published

2011-01-10

How to Cite

Pisarenko, N. A., & Shpakova, N. M. (2011). Effect of Amphiphiles on Hypertonic Hemolysis of Erythrocytes, Modified with Temperature of 49°C. Problems of Cryobiology and Cryomedicine, 20(4), 388–395. Retrieved from https://journal.cryo.org.ua/index.php/probl-cryobiol-cryomed/article/view/169

Issue

Vol. 20 No. 4 (2010): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 N. A. Pisarenko, N. M. Shpakova

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